54 results about "Streptomyces thermoautotrophicus" patented technology

The invention relates to preparation methods and application of streptomyces parvulus OUCMDZ-2554 bacterial strain and a product actinomycin D thereof. The preservation number of the bacterial strain is CGMCC7907. The streptomyces parvulus OUCMDZ-2554 has high yield of single compound actinomycin D, and the yield is 0.25mg / mL. The bacterial strain obtained at home for the firstly time has high yield, the culture medium for the bacterial strain only uses one nutritional ingredient, so that the cost is low, the preparation method is simple and easy to operate, the fermentation period is short, a large quantity of active substances can be produced by fermentation in large scale, the problem of medicine source is fundamentally solved, and the bacterial strain can offer a precursor for structural modification of the compounds and study on structure-activity relationship.

The invention discloses a streptomyces sp. and a screening method and application thereof, and belongs to the field of microbiology. In order to improve the resistance of tomatoes to plant diseases, the invention provides a streptomyces sp. strain named NEAU-HV9 with a strain preservation number of CGMCC 19436. The invention also provides application of the strain in inhibiting activity of pathogenic bacteria causing plant diseases and producing actinomycin D, and the streptomyces sp. strain NEAU-HV9 can be used for preparing microbial agents which can be used for inhibiting plant tomato bacterial wilt. The invention provides a high-quality material for the research and development of environment-friendly actinomycetic inoculant.

The present invention provides a method for enhancing the gene editing efficiency of actinomycetes and its application. Taking the deletion of actII-ORF4 in Streptomyces coelicolor as an example, the inducible promoter tipAp, ribosome switch and split Cas9 are used to control the activity of Cas9. Compared with the traditional constitutive Cas9 expression, the conversion efficiency is increased by about 260 times in the conversion process; the editing process adds inducers and increases the ATP concentration, so that the editing efficiency reaches 80%. The method of the present invention establishes a CRISPR / Cas9 system that can be induced by small chemical molecules and reversibly regulated by blue light, so as to ensure that the transformation efficiency of the plasmid and the normal growth and metabolism of the target host are not affected to the greatest extent, and realize the double-stranded The precise and high-efficiency gene editing of fragmentation, the invention is applied to the genetic engineering and genetic modification of other models including Streptomyces or industrial actinomycetes.

A streptomycesfradiae DL2-F-5 is separated from marine or fresh water microbe beneficial bacterial group. It can produce antibacterial substances or increase immune active ingredients, prevent aquatic animal diseases, avoid destruction for purified water environment by antiseptics, inhibit pathogenic bacteria multiplying, decompose organic pollutant and residual baits in water body and improve water-body environment. It can be used in medicine, food and feed additives production.

Disclosed is a compound useful as an active ingredient for a pharmaceutical composition having an a-amylase-inhibiting activity, particularly a pharmaceutical composition for the treatment of diabetes. Studies have been made for discovering a compound having an a-amylase-inhibiting activity among the compounds produced by Streptomyces sp. Strain 6982 which is a ray fungus belonging to the genus streptomyces, and it is confirmed that an aminosugar compound has an a-amylase-inhibiting activity. The aminosugar compound has an a-amylase-inhibiting activity, and therefore can be used as a prophylactic or therapeutic agent for diabetes, obesity, NASH (non-alcoholic steatohepatitis), particularly as an ameliorating agent for postprandial blood glucose elevation.

The invention discloses a compound microbial bacterial agent used for coal gangue artificial ecological matrix, including 5 kinds of actinomycetes: Streptomyces violaceum ( Streptomyces thermoviolaceus ), Streptomyces thermophilus ( S.thermodiastaticus ), Streptomyces carbon monoxide ( S.thermocarboxydus ), Streptomyces albus ( S. albidoflavus ) and Streptomyces vulgaris ( S. thermovulgaris ), 1 bacteria: Pseudomonas ( Pseudomonas sp.), and 1 mold: Penicillium oxalicum ( Penicillium oxalicum ). The preparation process of the bacterial agent includes four steps: activation of the bacterial strain, preparation of the seed liquid, preparation of the liquid composite bacterial agent, and preparation of the solid bacterial agent. The technology of the invention solves the problem that the coal gangue lacks microorganisms and the nutrients are not conducive to plant absorption and utilization, and provides a new way for the resource utilization of the coal gangue and landscaping waste.

The invention discloses a high-temperature-resistant actinomycete which can efficiently degrade and convert rice straw and nitrogen in pig manure. The actinomycete disclosed by the invention belongs to streptomyces griseorubens with the preservation number of CGMCC No. 5706. The actinomycete can strongly degrade methyl cellulose in the rice straw, further can quickly convert nitrate nitrogen and organic nitrogen in the pig manure, and tolerates the high temperature of more than 70 DEG C; the actinomycete can be added to the mixed compost of rice straw and pig manure to obviously rise the compost temperature, shorten the fermentation cycle, and improve the quality and the yield of the organic fertilizer; in addition, the organic fertilizer inoculated with the solid bacteria agent can quickly fertilize soil, so as to increase the content of the organic fertilizer, total nitrogen, nitrate nitrogen and ammonium nitrogen, activate the soil enzyme activity, and finally increase the yield of the crop.

The invention relates to a method of preparing protein hydrolysate of chilled Antarctic krill. The method comprises the following steps: (1) mixing chilled Antarctic krill raw material with water in a ratio of 1-3L water per kilogram raw material, grinding and homogenizing, adding alkaline protease, and performing enzymolysis for 1-5 hours at the temperature of 35-55 DEG C; (2) performing solid-liquid separation to obtain clear liquor, concentrating and drying the clear liquor to obtain the protein hydrolysate, wherein the content of fat is 0%, and the total content of peptides and amino acid is more than 60%. The total yield of actinomycin produced through fermentation by virtue of streptomycete in an Antarctic krill protein hydrolysate group is equivalent to the yield of the existing commercialization nitrogen source group, and high performance liquid chromatography-mass spectrum analysis results show that the diversity of analogue of actinomycin D produced through fermentation in the Antarctic krill protein hydrolysate group is better.

The invention discloses a microbial agent which adopts bacillus subtilis CGMCC1. 3382, dactylosporangium sp. ACCC No. 40661 and streptomyces roseoflavus ACCC No. 40400 as preparation bacteria; all bacteria are prepared to form fermentation liquors, the fermentation liquors are mixed, and the mixture is subjected to submerged fermentation to prepare the microbial agent. The microbial agent utilizes the interaction among microorganism in a reasonable manner, has the characteristics of high adaptability, excellent stability, and high antagonism to pathogenic bacteria, and difficulty in aging, can realize efficient antagonism to the potato common scab, stimulate growth of the potatoes, and improve the quality of the potatoes so as to achieve the purposes of production increasing and high yield.

A streptomycesfradiae DL2-F-5 is separated from marine or fresh water microbe beneficial bacterial group. It can produce antibacterial substances or increase immune active ingredients, prevent aquatic animal diseases, avoid destruction for purified water environment by antiseptics, inhibit pathogenic bacteria multiplying, decompose organic pollutant and residual baits in water body and improve water-body environment. It can be used in medicine, food and feed additives production.

The invention belongs to the technical field of metabolite extraction, and discloses a method for separating and extracting secondary metabolites of streptomyces, which comprises the following steps:carrying out elution on a fermentation broth by using an HP-20 resin column, performing chromatographic separation by a silica gel column and a Sephadex LH-20 gel column, and performing analysis and purification of a compound by (semi) preparing a high performance liquid chromatography. Modern instrument spectrum analysis technologies such as nuclear magnetic resonance and high-resolution mass spectrometry are used for identifying the structure of the monomeric compound. According to the invention, actinomycetes which are separated from a soil sample and have antibacterial and anti-tumor cellactivity are fermented, and induction research is carried out on bioactivity of metabolites and part of products generated by the actinomycetes, so that diversity of microbial compounds is enriched; areference and theoretical basis is provided for research and exploration of active substances produced by other actinomycetes; meanwhile, a premise is provided for exploring and developing active natural compounds and commercial drugs.