123 results about "Streptomyces hygroscopicus" patented technology

The invention provides a novel microbial fertilizer special for tobaccos. The fertilizer comprises the following components in parts by weight: 5 to 25 parts of a complex microbial inoculant and 75 to 95 parts of a carrier, wherein the complex microbial inoculant contains 1.6 to 8 hundred million cfu / g of bacillus subtilis, 1.6 to 8 hundred million cfu / g of bacillus licheniformis, 0.4 to 2.0 hundred million cfu / g of trichoderma harzianum and 0.4 to 2.0 hundred million cfu / g of calicheamicin streptomyces hygroscopicus; the carrier is a powdered material which are made by performing microbe fermentation and decomposition on rape seed cakes, bean pulp cakes and sesame-seed cakes, and then performing drying and sieveing. The microbial fertilizer contains bacterial strains for antagonizing fungus pathogenic bacteria, bacterial strains for preventing and curing the breeding of soil pathogenic bacteria, bacterial strains for improving the soil environment and the like. The carrier is fermented three-cake fertilizer. The invention also provides a preparing method for the microbial fertilizer. The microbial fertilizer special for the tobaccos, provided by the invention, is powder, is capable of absorbing the water and preserving the moisture. The fertilizer is mainly used as the base fertilizer when fertilizing, the soil fertility can be improved, the nutrient absorption of crops is improved, the diseases of a plurality of crops can be prevented, and the quality and the yield of tobacco leaves can be effectively improved.

A streptomyce shygeroscopicus (CCTCC No.M203062) able to generate glutamine transaminase wigh high output is extracted from the soil of milk cow farm through plate screening, primary fermenting, flask culture, comparing test with standard color tape, and enzyme activity test. Said glutamine transaminase is prepared by deep fermentation.

The invention relates to an environment-friendly straw fermenting agent. The environment-friendly straw fermenting agent is prepared from the following seven raw materials in percentage by weight: 25 percent of bacillus subtilis, 15 percent of trichoderma viride, 18 percent of saccharomyces cerevisiae, 12 percent of aspergillus niger, 10 percent of streptomyces hygroscopicus, 10 percent of trichoderma koningii and 10 percent of arthrobacter. The environment-friendly straw fermenting agent can accelerate the decomposition speed of cellulose and can completely decompose crop straws within aboutten days to return to the field, so that the straws are corroded, blackened and composted. The environment-friendly straw fermenting agent can accelerate release of nutrient elements in the straws, provides more nutrients for growth of the crops at the later stage, maintains and improves soil fertility, improves soil organic matter, improves physicochemical property of the soil, increases the yield of the crops by about 10 percent, and can be widely applied to returning straws of the crops such as rice, wheat, corn and the like to the field.

The invention relates to a liquid composite biological multi-control fertilizer. The invention is characterized in that the liquid composite biological multi-control fertilizer is prepared from the following raw materials in parts by weight: 1-3 parts of Streptomyces hygroscopicus solution, 1-3 parts of Streptomyces cinnamofuscus solution, 2-4 parts of Bacillus amyloliquefaciens solution, 2-4 parts of bacillus sphaericus solution, 7-15 parts of Bacillus mucilaginosus solution, 1-3 parts of Azotobacter chroococcum solution, 1-3 parts of Streptomyces aureochrogmoenes, 8-10 parts of Trichoderma viride fermentative degradation solution, 8-10 parts of matrix and 45-65 parts of sterilized water. The invention prepares a crop-absorbing carbohydrate and organic matter Trichoderma viride fermentative degradation solution, and provides high-efficiency beneficial microbial florae with phosphate solubilizing, potassium solubilizing, nitrogen fixation and disease resistance effects. The fertilizer can simultaneously perform the three functions of biological pesticide, biofertilizer and biological nutrients, and has the advantages of no toxicity or harm and no environment pollution. Compared with other fertilizers with the same nutrients, the crop yield is increased by 20%.

The invention discloses a streptomyces hygroscopicus and applications thereof. The streptomyces hygroscopicus strain is preserved in China Center for Type Culture Collection with CCTCC NO. of M 207200 and the preservation date of December 14th, 2007. The strain with CCTCC NO. of M 207200 is a currently-separated natural strain with high sirolimus yield and excellent fermentation performance. The fermentation yield of the sirolimus-producing strain can reach over 600ug / ml, has relatively less fermentation byproducts, lowers post extraction difficulty, can reach the product yield of over 30 percent, and is applicable to industrial production.

This invention provides Streptomyces anhygroscopicus var. wuyiensis F64 (CGMCC No.1967), which can be used to prepare a novel biological pesticide, wuyiencin with the fermentation solution titer higher than 5000 U / mL, in conventional culture conditions. This invention also optimizes the culture method and the culture medium formula, by which the fermentation solution titer can reach 6000-6500 U / mL. This invention can shorten the fermentation time by 5-10 h, increase the fermentation solution titer by 150-200%, increase the yield by 100% and improve the disease-preventative effect by 15-20%.

The invention relates to a genetic engineering strain for increasing the yield of ascomycin and a constructing method thereof. The genetic engineering strain is streptomyces hygroscopicus var.ascomyceticus TD01, and is deposited in the China General Microbiological Culture Collection Center with an accession number of CGMCC 10615. By cloning fkbN and frr genes, an fkbN-frr double-gene tandem overexpression vector is constructed by utilization of escherichia coli-streptomycete shuttle plasmid pIB139 and is transformed into escherichia coli ET12567, and streptomyces hygroscopicus var.ascomyceticus is transformed by a conjugal transfer method to allow the overexpression vector to be expressed in an original strain. The yield of the ascomycin of the constructed ascomycin genetic engineering strain TD01 reaches 500 mg / L, and is increased by 42% than that of the original strain. The genetic engineering strain and the constructing method have high potential and application value.

The invention relates to a transglutaminase zymogen gene of Streptomyces hygroscopicus and the expression thereof, pertaining to molecular biology field. The invention provides the gene sequence of transglutaminase zymogen of Streptomyces hygroscopicus CCTCC M203062 (China patent: 03152956.9, 2003), and further provides a vector system and a host system that has soluble express of the zymogen of the gene code, a part of soluble expressed zymogen can be obtained through the expression system, and the zymogen can be treated by trypsin to prepare activated transglutaminase.

The invention discloses streptomyces hygroscopicus capable of producing salicylic acid and rapamycin and application of the streptomyces hygroscopicus to prevention and control of plant oomycetes andfungal diseases. The invention also discloses the streptomyces hygroscopicus capable of producing the salicylic acid and the rapamycin, which has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 15518, and the application of the streptomyces hygroscopicus to the prevention and control of the plant oomycetes and fungal diseases. The invention identifiesa biocontrol bacterium aiming at the plant oomycetes and fungal diseases, especially phytophthora infestans and fusarium oxysporum; the bacterium can be used for remarkably inhibiting spore germination and mycelium growth of the phytophthora infestans; an effective method is provided for biologically preventing and controlling the plant oomycetes and fungal diseases, especially the phytophthora infestans and fusarium wilt. The method disclosed by the invention can be used for effectively preventing and controlling the phytophthora infestans, the fusarium wilt and other plant diseases and reducing the economic loss; the method has important meaning of reducing the utilization of pesticide and alleviating environment pollution, and also has significant application value of keeping the balance and sustainable development of an ecological system.

The invention discloses antagonistic actinomycete for preventing and controlling continuously cropped strawberry root rot and a preparation method of a biocontrol agent of the antagonistic actinomycete. Bacterial strain B04 belongs to streptomyces hygroscopicus (Streptomyces hygroscopicus), with the strain preservation number of CGMCC NO.9918. The biocontrol strain B04 is separated from a healthy strawberry root, and has a strong inhibiting effect on pathogenic bacteria, such as fusarium, verticillium dahliae, rhizoctonia, phytophthora and the like, causing strawberry soil-borne root diseases. The antagonistic actinomycete prepared by taking wormcast and wheat bran as the main materials of fermentation medium can completely meet the nutritional requirements produced by solid fermentation of biocontrol antinomycetes. Compared with conventional fermentation, the production cost of the preparation method is reduced by 30 to 50 percent, and the preparation method is beneficial for popularization and application of the biocontrol agent of the antagonistic actinomycete. After the biocontrol agent of the antagonistic actinomycete is applied to continuously cropped soil, the biological control rate reaches greater than 70 percent, and has great significance on preventing and controlling the soil-borne diseases of strawberry, improving the quality of agricultural products and protecting the environment.

The invention discloses a glutamine transaminase high-yielding strain and application thereof. The strain is named as streptomyces hygroscopicus NYU-70 according to classification and naming, and has been preserved in China Center for Type Culture Collection and preservation number of CCTCCM2014121. Streptomyces hygroscopicus NY-1 is used as a starting strain for low energy ion implantation mutagenesis, activity of the enzyme of the strain after mutagenesis is measured through casein gel method and colorimetric method, mutant strains with high activity in producing glutamine transaminase are screened out as the starting strains for the next round of mutagenesis, and the above steps are repeated to obtain the glutamine transaminase high-yielding strain. The strain has high activity and good genetic stability in fermentation production of glutamine transaminase. The mutant strain can be used in liquid shake flask fermentation to obtain glutamine transaminase, and has enzyme activity of 16.9U / mL.

The invention discloses a high-efficiency and energy-saving validamycin fermenting method, belonging to the field of microbial fermentation technology. The method comprises the following technological steps: 1) inoculating streptomyces hygroscopicus strains into liquid fermentation culture medium and controlling the inoculum size to be 8-15%, wherein the fermentation temperature is at 35-45 DEG C; and 2) controlling the ventilator capacity to be 2500-3500m<3> / h at early fermentation stage, fermenting for 25-35h, and then controlling the dissolved oxygen to be 20-50% until the fermentation is finished. According to the method, the dissolved oxygen rule can be discovered by measuring and analyzing the dissolved oxygen, and the concentration of the dissolved oxygen is controlled to be 20-50% after the fermentation culture is conducted for 25-35h, so that the energy consumption of an air compressor can be effectively reduced, the total air usage amount can be saved by about 25%, the electricity consumption can be saved by 10% of total electricity consumption each year, and the production volume of the validamycin can be improved by more than 4%.

The present invention discloses gene engineering bacteria highly expressing transglutaminase, and applications thereof, wherein a cleavage site Lys-Arg of yeast endogenous Kex2 protease is introduced between a proenzyme region gene and a maturase region gene of the expressed streptomyces hygroscopicus CCTCC M203062 transglutaminase proenzyme, and the proenzyme is expressed in Yarrowia lipolytica by using the expression vector. According to the present invention, the gene engineering bacteria is adopted to carry out fermentation to produce transglutaminase so as to achieve independent activation of the transglutaminase without an exogenous protease effect, effectively reduce production cost, and simplify production steps.

The invention belongs to the organic fertilizer technical field, in particular relating to a soil improvement type composite biological organic liquid fertilizer. The soil improvement type composite biological organic liquid fertilizer is prepared by combination of bifidobacterium, lactobacillus bulgaricus, lactobacillus casei, saccharomyces cerevisiae, bacillus subtilis, streptomyces hygroscopicus and so on. The compositions are all profitable strains sieved from soil, are cultured and multiplied by application of the biotechnology, added with organic substances and nutritive elements, and processed into the novel composite biological organic liquid fertilizer by means of chelating, and are favorable for recovering the ecosystem of soil organisms. Therefore, the product of the soil improvement type composite biological organic liquid fertilizer not only has the function of fertilization but also can improve the physical property and the biological property of the soil and have the function of improving the soil, and can be widely used for various crops.

The present invention relates to a liquid compound biological preservative. The liquid compound biological preservative is characterized by consisting of the following raw materials in parts by weight: 5-25 parts of chitosan, 5-25 parts of epsilon-polylysine, 5-25 parts of lysozyme, 5-20 parts of streptomyces hygroscopicus liquid, 5-20 parts of streptomyces microflavus liquid, 5-20 parts of streptomyces aureochromogenes liquid, 5-20 parts of lactic streptococci liquid, 5-20 parts of bacillus subtilis liquid, 5-20 parts of bacillus licheniformis liquid, 5-20 parts of beer yeast liquid, 5-20 parts of paecilomyces lilacinus liquid and 5-20 parts of trichoderma harzianum liquid. The liquid compound biological preservative is high-efficient, non-toxic, healthy and nutritious, beneficial for food safety, and especially has significant effects on preservation of nanguo pears, actinidia arguta or strawberries.

The invention discloses a transglutam kinase coding gene interrupted streptomyces hygroscopicus and an application thereof. The invention mainly adopts a homologous recombination technique, and makes transglutam kinase coding genes subjected to insertional inactivation through single exchange so as to obtain recon not producing transglutam kinase. The differentiation of the streptomyces hygroscopicus obtained by the invention is interrupted without influencing growth of substrate myceliums. A genetically engineered microorganism constructed by using the interrupted streptomyces hygroscopicus as a host is used for producing the transglutam kinase. The interrupted streptomyces hygroscopicus can be used as a good streptomyces hygroscopicus expressing host. Metabolin changes caused by the differentiation process in the ferment process of streptomyces hygroscopicus can be solved, the ferment production quantity and stability can be improved, subsequent streptomyces hygroscopicus gene change and microorganism physiology study can be greatly promoted, and a new type strain is provided for production and ferment of streptomyces hygroscopicus.

The invention discloses a Streptomyces angustmyceticus strain CQUSa03, and a preservation number is CGMCC No. 18715. The invention also discloses a biological agent taking the strain CQUSa03 as an active ingredient. The invention also discloses an application of the strain CQUSa03 in prevention and treatment of plant oomycetes and plant fungal diseases, and an application of the strain CQUSa03 inpromotion of plant growth. The invention also discloses a preparation method of a liquid inoculant containing the strain CQUSa03. The preparation method comprises the following steps: (1) activating the strain; (2) performing seed culture; and (3) performing liquid fermentation; adding a seed culture medium into a fermentation culture medium for fermentation to obtain a liquid microbial agent; wherein a formula of the fermentation medium comprises 4% of glucose, 1% of soybean cake powder, 0.2% of amino acid powder, 0.5% of K2HPO4, 0.5% of KH2PO4, 0.5% of NaCl, 0.15% of ferrous sulfate, 0.2% ofan antifoaming agent and a pH value is 7.2-7.4.

The invention relates to a method for producing Sirolimus by using streptomyces hygroscopicus, which comprises: inoculating streptomyces hygroscopicus into a seed culture medium, and performing activated culture for 60 hours under a condition that the temperature is 30 DEG C; inoculating the streptomyces hygroscopicus into a fermentation tank according to an inoculation amount of 6 weight percent; at the early stage of fermentation, namely at the growth stage of bacteria, fermenting for 8 hours, performing exponential feeding of glucose, controlling a specific growth rate to be between 0.02 to 0.06 hours, performing high-cell density culture, and obtaining high bacterium concentration; and after fermentation lasts 32 hours, namely in the rear middle stage of the fermentation, and providing a precursor for synthesizing Sirolimus by constant-speed feeding of shikimic acid and L-lysine, which are precursors to improve fermentation level. Compared with the prior art, the peak titer of the Sirolimus synthesized by the method reaches 700mg / L, which is 30 percent higher than that synthesized by batch fermentation. According to the fermentation policy, the control is simple and the yield of Sirolimus is high.

The invention discloses a biological protein preparation for preventing and controlling citrus huanglongbing and a preparation method for the biological protein preparation, and belongs to the technical field of biopesticides. According to the adopted technical scheme, the biological protein preparation is formed by compounding NAC protein and a streptomyces hygroscopicus metabolite. The preparation method comprises the following steps: (1) selecting a streptomyces hygroscopicus strain, performing activation, culture expansion and fermentor culture on the streptomyces hygroscopicus strain, and stopping fermentation when the concentration of a thallus reaches 60-80 percent to obtain streptomyces hygroscopicus; (2) performing cell disruption on the streptomyces hygroscopicus obtained in the step (1), and concentrating to remove 70-90 percent of the volume of water to obtain a condensed fermentation broth; (3) in parts by mass, weighing 20-40 parts of the condensed fermentation broth, 1-1.5 parts of NAC protein, 20-30 parts of a carrier and 5-8 parts of an additive, uniformly mixing, drying and grinding. The preparation is friendly in environment, simple in preparation process, non-toxic and high in efficiency, has a good prevention and control effect on the citrus huanglongbing, enables leaves of infected plants to become green from yellow, and has a certain recovery effect on the infected plants.

The invention provides an engineering strain with high yield of FK520 (ascomycin). A gene expression cassette is integrated in a genome of the strain, and the gene expression cassette expresses a protein encoded by a biosynthetic gene of ethyl malonyl coA. The yield of FK520 can be increased substantially by the transformed strain, and the proportion (mass ratio) of the main product FK520 to sideproduct FK523 is increased.

The present invention relates to a process of preparing geldanamycin by culturing Streptomyces hygroscopicus in an aqueous nutrient medium. The aqueous nutrient medium can comprise starch, a starch conversion enzyme, an assimilable source of protein and optionally a further assimilable source of carbon or can comprise an assimilable source of carbon and an assimilable source of protein. The present invention also relates to a process for the isolation and purification of geldanamycin.

The invention discloses a method of preparing ascomycin through fermentation with streptomyces hygroscopicus. On the basis of the difference on metabolism between on a fermentation pot and a fermentation bottle of the ascomycin in the prior art, a material supplying process for the fermentation bottle is disclosed, namely, various acids or carbon sources are supplied during the middle stage of fermentation to control the pH of a fermentation liquid, to form similar metabolism status between the fermentation pot and the fermentation bottle, thereby increasing fermentation unit and reducing production cost. The method is simple in operation, is mild in conditions. The raw materials are wide in sources and low in cost. The method is low in demand on equipment and is suitable for industrial production.

The invention discloses a method for breeding an high-yield ascomycin strain by performing femtosecond laser mutagenesis on streptomyces hygroscopicus ascomycota subspecies and a culture medium preparation method; the method comprises the following steps: at room temperature, mixing mature slant spores of the streptomyces hygroscopicus ascomycota subspecies (ATCC14891) and sterile water to obtain monospore suspension containing 106-107 spores per milliliter; irradiating the spore suspension for 1-10 min by using the titanium sapphire femtosecond laser with centre wavelength of 800 nm, pulse width of 150 fs and frequency of 76 MHz under a irradiation power of 10-30m W; properly diluting the spore suspension, coating on a solid panel to culture, then screening by using a shake flask to obtain a high-yield ascomycin mutation strain. The method provided by the invention has the advantages that the used femtosecond laser mutagenesis method is feasible and safe to operate; the mutation effect is better than that of the traditional physical and chemical mutagenesis method, and the femtosecond laser mutagenesis method has great popularization value in breeding of microorganism pharmaceutical strains; by using the femtosecond laser irradiation to perform the mutagenesis, the high-yield ascomycin mutation strain can be bred. The positive mutation rate of the mutation strain is 5-30%, and fermenting unit is improved by 10-60% in comparison with that of an original strain.

The invention discloses a method for separating and purifying a transglutaminase-activating protease inhibitor, which belongs to the technical field of separation and purification of biological products. The present invention uses Streptomyces hygroscopicus as the starting strain, and the transglutaminase activating protease inhibitory factor prepared by liquid submerged fermentation obtains electrophoretic pure transglutaminase after ethanol precipitation, cation exchange chromatography and hydrophobic chromatography Enzyme-activating protease inhibitor solution for subsequent protein sequencing and characterization studies. The method has simple steps and high enzyme purity, and is a simple and effective enzyme separation and purification method.

The invention provides a method for preventing and treating rice diseases and insect pests. In a seedling culture stage, acalypha australis and tine peas are added, so that the weeding effect on rice seedlings can be improved; a small amount of agastache rugosus powder is added, and a dispelling effect is achieved on some pests due to the special fragrance; calcium superphosphate is added into soil in advance to cause an alkalescence environment for soil, and a killing effect is achieved on some bacteria; spore streptomyces hygroscopicus with a good antibacterial effect is matched with stillage for fermentation, and the antibacterial effect is better after fermentation by means of the environment suitable for fermentation in the stillage. No chemical weeding agent or pesticide is used, and the method is environmentally friendly and free of pollution.

The invention provides a compound continuous-cropping-resistant biological fungicide special for ginsengs / Chinese yams / peanuts and a preparation method thereof. The compound continuous-cropping-resistant biological fungicide is prepared from sheep manure, short and small bacillus pumilus, bacillus amyloliquefaciens liquid, bacillus sphaericus liquid, bacillus licheniformis liquid, paecilomyces fumosoroseus liquid, penicillium oxalicum liquid, streptomyces hygroscopicus liquid, streptomyces griseochromogenes liquid and an auxiliary matrix. The preparation method comprises the steps that 1, the bacterial liquids are prepared respectively and are mixed evenly; 2, main and auxiliary matrixes are evenly mixed, and then drying is performed under the pressure of 30-50 DEG C. The biological fungicide is resistant to continuous cropping, fixes nitrogen, dissolves phosphorus, releases potassium, absorbs moisture, keeps soil humidity, promotes decomposition of soil organic matter, is bacteriostatic and insect-resistant, improves micropopulation in soil, improves the functions of a soil structure, does not pollute the environment and is free of toxicity and residues and safe to human.

The invention provides a microbial agent for preventing and treating apple ring rot and a preparation method thereof. The microbial agent is obtained by using a gel carrier loaded with compound microbial powder. The compound microbial agent comprises bacillus cereus ACCC03288 powder, trichoderma harzianum ACCC30371 powder, pseudomonas chlororaphis ACCC19853 powder, paecilomyces lilacinus ACCC30639powder and thorn spore streptomyces hygroscopicus ACCC40018 powder. The growth and reproduction of apple ring rot germs are synergistically inhibited, nutrition support is provided for apple trees, and the growth of apples is promoted. The gel carrier is obtained by utilizing a cross-linking reaction of acrylamide and sodium acrylate, cross-linking sodium alginate and calcium chloride and introducing polyvinyl alcohol, can be naturally degraded, has a slow release effect on the compound microbial powder, and provides nutrition support for the microbial growth, so that the compound microbial powder has a better control effect. The microbial agent only needs to be applied once, thereby reducing the labor intensity.

The invention provides a process for preparing rapamycin by using a microbial fermentation method. The method comprises the following steps of: culturing streptomyces hygroscopicus of which the collection number is CGMCC No.5145 in a slant culture medium, and inoculating into a fresh seed culture medium for culturing; and inoculating 2 percent of strain cultured by using the seed culture medium into a fresh fermentation culture medium for fermenting and culturing to obtain a fermentation liquor containing rapamycin, and separating and purifying the fermentation liquor containing the rapamycin according to the solubility difference of the rapamycin in water and an organic solvent. In a method provided by the invention, a production technology for producing high-purity rapamycin at low cost is provided. By reducing the cost of a raw material, an immunosuppressive medicament which is economically acceptable, safe and effective can be provided for an organ transplantation patient. The product purity of the rapamycin prepared with the process is over 95 percent, and the recrystallization yield is over 84 percent.

The invention, belonging to the technical field of agricultural microbiology, specifically relates to an anti-Rhizoctonia solani Streptomyces hygroscopicus Sh-43 strain, its fermentation solution production method and application. Streptomyces hygroscopicus Sh-43 strain is preserved in China Center for Type Culture Collection in Wuhan University under accession Number of CCTCC NO: M2012172 on May18, 2012. The Streptomyces hygroscopicus Sh-43 strain of the invention has the advantages of significant anti-Rhizoctonia solani effect, wide antimicrobial spectrum, stable resistance effect, simple culture conditions, and good control effect on rice sheath blight, and has wide application prospect in the field of biological control of plant diseases.

Macrolactams are made by feeding aromatic amino acids as replacement starter units to a mutant strain of the geldanamycin-producing microorganism Streptomyces hygroscopicus var. geldanus NRRL 3602, wherein the gene cluster encoding enzymes for the biosynthesis of the natural starter unit 3-amino-5-hydroxybenzoic acid has been deleted.