The invention discloses a recombinant adenovirus and tetravalent
adenovirus vaccine and a preparation method thereof. The tetravalent recombinant
adenovirus vaccine contains a recombinant type 3 adenovirus strain, a recombinant type 7 adenovirus strain, a recombinant type 14 adenovirus strain and a recombinant type 55 adenovirus strain. The preparation method disclosed by the invention comprises the following steps: preparing recombinant shuttle plasmids containing hexon
gene segments, and performing in-
bacteria homologous recombination with a recombinant
human type 3 adenovirus strain, thereby obtaining a recombinant adenoviral
genome in which the hexon
gene segments are replaced by type 7, type 14 and type 55; transfecting cells, rescuing to obtain recombinant
human type 3, 7, 14 and 55
recombinant adenoviruses with different main
capsid protein-hexon proteins; purifying, mixing according to the same
protein content, and inactivating by using beta-
propiolactone, thereby obtaining the tetravalent
adenovirus vaccine. The tetravalent adenovirus vaccine is capable of inducing
neutralizing antibody responses to four types of
serotype adenoviruses, and the neutralizing
titer is 500-1000.