The invention relates to a recomposed
escherichia coli base
cell for efficient synthesis of
terpene chemical compounds as well as a preparation method and application thereof. Particularly, an
escherichia coli endogenous 2-C-methyl-D-
erythritol-4-
phosphoric acid (MEP) precursor pathway is reconstructed; the reconstructed
escherichia coli base
cell is utilized to perform the efficient biological synthesis of the
terpene chemical compounds; the reconstruction of the precursor pathway mainly comprises the steps of fully digging the MEP precursor pathway
gene modules of the sources of other natural microorganisms, screening
gene modules with excellent characteristics to be expressed in the
escherichia coli, and at the same time, performing the downstream synthesis pathway for integrally assembling the colibacillus chemical compounds in the reconstructed base
cell, wherein the colibacillus chemical compounds include
sesquiterpene chemical compounds such as amorphadiene,
diterpene chemical compounds such as shell
alkene, tetraterpenes chemical compounds such as
lycopene, polyterpene chemical compounds, other
terpene alkaloid chemical compounds and the like. The
escherichia coli base cell can remarkably facilitate the synthesis of the terpene chemical compounds.