The invention relates to a method for detecting sulfonamide residues in eel by pressurized
capillary electrochromatography, which is characterized in that the method comprises the following steps: extracting a sample by
ethyl acetate, purifying the sample by a HLB
solid-phase extraction column, and filtering the sample by a 0.22-micron filter membrane, performing analysis by pressurized
capillary electrochromatography, performing detection at 270 nm with an
ultraviolet detector by using a capillary electrochromatographic column with reversed-phase bonds and C18 as fillers as a detection column, wherein the mobile phase is
acetonitrile and
sodium dihydrogen
phosphate (5 mmol / L, pH 6.0) with a ratio of 30:70 (V / V). The invention performs pretreatment of the sample by using a classic sample treatment method of liquid-liquid extraction combined with
solid-phase extraction, and effectively reduces the content of impurities in fish
flesh tissue and matrix interference; the
capillary electrochromatography is adopted for analysis and detection, which has less organic
reagent consumption; with the application of an external
voltage, the
retention time of 5 sulfonamides is reduced; the sensitivity is improved; 5 sulfonamides are separated and detected simultaneously; the method of the invention not only meets routine detection of eel sulfonamide residual quantities, but also is applicable to the detection of sulfonamides in other
aquatic product samples.