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Preparation method of graphene-modified capillary-column chromatography

A technology of capillary electrochromatography and capillary column, which is applied in the field of capillary electrochromatography, can solve the problems of complicated preparation process and short service life, and achieves the effects of easy availability of raw materials, long service life and simple method.

Active Publication Date: 2013-05-29
UNIMICRO SHANGHAI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method has a long lifetime, it requires a tedious preparation process
Another method is to use the dynamic coating method, which is simple and repeatable, but its service life is very short

Method used

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  • Preparation method of graphene-modified capillary-column chromatography
  • Preparation method of graphene-modified capillary-column chromatography
  • Preparation method of graphene-modified capillary-column chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A preparation method of a graphene-modified capillary electrochromatographic column, the specific steps comprising the following steps:

[0034] (1) Pretreatment of capillary;

[0035] Rinse the capillary with deionized water for 10 minutes, then rinse the capillary with NaOH solution for 4 hours, and then rinse with deionized water for 10 minutes;

[0036] (2) polyelectrolyte modified capillary inner wall;

[0037] Select a cationic electrolyte, prepare 8% (w / v) polydiallyldimethylammonium chloride (PDDA) solution, and add 0.5M salt solution to it to form a cationic polyelectrolyte coating solution;

[0038] Inject the above prepared liquid into the pretreated capillary column within 5 minutes, and then rinse the capillary with deionized water for 5 minutes to form a cationic capillary column;

[0039] (3) Preparation of graphene-modified capillary column;

[0040] Graphene oxide and deionized water are prepared according to the ratio of 0.2%wt graphene oxide deposi...

Embodiment 2

[0049] All the other are identical with above-mentioned embodiment, difference is, a kind of preparation method of graphene modified capillary electrochromatographic column, concrete steps comprise as follows:

[0050] (1) Pretreatment of capillary;

[0051] Rinse the capillary with deionized water for 10 minutes, then rinse the capillary with NaOH solution for 4 hours, and then rinse with deionized water for 15 minutes;

[0052] (2) polyelectrolyte modified capillary inner wall;

[0053] Select a cationic electrolyte, prepare 8% (w / v) polydimethyldiallylammonium chloride solution, and add 0.5M KCl solution to it to form a cationic polyelectrolyte coating solution;

[0054] Inject the above prepared liquid into the pretreated capillary column within 5 minutes, and then rinse the capillary with deionized water for 5 minutes to form a cationic capillary column;

[0055] (3) Preparation of graphene-modified capillary column;

[0056] Graphene oxide and deionized water are form...

Embodiment 3

[0059] All the other are identical with above-mentioned embodiment, difference is, a kind of preparation method of graphene modified capillary electrochromatographic column, concrete steps comprise as follows:

[0060] (1) Pretreatment of capillary;

[0061] Rinse the capillary with deionized water for 5 minutes, then rinse the capillary with NaOH solution for 3 hours, and then rinse with deionized water for 5 minutes;

[0062] (2) polyelectrolyte modified capillary inner wall;

[0063] Select a cationic electrolyte, prepare a 5% (w / v) acrylamide acryloyloxyethyltrimethylammonium chloride copolymer P (AM AETAC)) solution, and add 0.1M NaCl solution to it to form a cationic Polyelectrolyte coating solution;

[0064] Inject the above prepared liquid into the pretreated capillary column within 3 minutes, and then rinse the capillary with deionized water for 5 minutes to form a cationic capillary column;

[0065] (3) Preparation of graphene-modified capillary column;

[0066] ...

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Abstract

The invention discloses a preparation method of graphene-modified capillary-column chromatography. Oxidized graphene groups are deposited on the inner wall of a capillary through cationic polyelectrolyte by using a layer by layer self-assembling method, so that the defects of complex chemical bonding method and short service life of a dynamically-coated column and the like are overcome. Raw materials are easily obtained; the method is simple, convenient and stable; and the prepared capillary-column chromatography is longer in service life.

Description

technical field [0001] The invention relates to the technical field of capillary electrochromatography, in particular to a preparation method of a graphene-modified capillary electrochromatography column. Background technique [0002] Capillary electrochromatography is an efficient and rapid micro-separation technology that has developed rapidly in recent years. It is a separation and analysis method with dual separation performance of capillary electrophoresis and chromatography. It combines the high efficiency of capillary electrophoresis with the high selectivity of high performance liquid chromatography. Capillary electrochromatographic columns are the core of capillary electrochromatographic technology, and the innovative development of new chromatographic columns has been a long-term focus and exploration in this field. [0003] Carbon materials have a strong adsorption capacity for organic matter, among which fullerenes, single-walled carbon nanotubes, multi-walled c...

Claims

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Application Information

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IPC IPC(8): B01D15/22B01J20/281B01J20/32
Inventor 阎超李静
Owner UNIMICRO SHANGHAI TECH
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