Method for detecting sulfonamide residue in eel by pressurized capillary electrochromatography

Abstract

The invention relates to a method for detecting sulfonamide residues in eel by pressurized capillary electrochromatography, which is characterized in that the method comprises the following steps: extracting a sample by ethyl acetate, purifying the sample by a HLB solid-phase extraction column, and filtering the sample by a 0.22-micron filter membrane, performing analysis by pressurized capillary electrochromatography, performing detection at 270 nm with an ultraviolet detector by using a capillary electrochromatographic column with reversed-phase bonds and C18 as fillers as a detection column, wherein the mobile phase is acetonitrile and sodium dihydrogen phosphate (5 mmol / L, pH 6.0) with a ratio of 30:70 (V / V). The invention performs pretreatment of the sample by using a classic sample treatment method of liquid-liquid extraction combined with solid-phase extraction, and effectively reduces the content of impurities in fish flesh tissue and matrix interference; the capillary electrochromatography is adopted for analysis and detection, which has less organic reagent consumption; with the application of an external voltage, the retention time of 5 sulfonamides is reduced; the sensitivity is improved; 5 sulfonamides are separated and detected simultaneously; the method of the invention not only meets routine detection of eel sulfonamide residual quantities, but also is applicable to the detection of sulfonamides in other aquatic product samples.

Description

technical field [0001] The invention belongs to the field of food safety-veterinary drug residue detection, and relates to an analysis method for detecting sulfa drugs in eels by using capillary electrochromatography, specifically, using capillary electrochromatography to simultaneously measure sulfadiazine and sulfamethoxine in fish meat , sulfaquinoxaline, sulfamethoxazole, sulfamethoxazole five kinds of sulfa drugs. Background technique [0002] Sulfonamide drugs refer to the general term of a class of drugs with p-aminobenzenesulfonamide structure, which is a class of chemotherapeutic drugs for the prevention and treatment of bacterial infectious diseases. Because of its wide antibacterial spectrum, anti-coccidial and cheap and easy to obtain, it is still widely used in veterinary clinics and animal husbandry. Sulfa drugs are a class of antibacterial drugs that are allowed to be used in a limited amount by the state and can be used by feed producers. At present, the na...

AI Technical Summary

Problems solved by technology

These methods have different advantages and disadvantages: the detection speed of microbiological method is fast, but the detection sensitivity and specificity are poor; the sensitivity and selectivity of thin layer chromatography are good, but the repeatability is not good enough; the biggest deficiency of capillary electrophoresis is its sample injection. The amount is small, which limits the sensitivity and cannot determine residues at the μg / kg level or lower concentrations

Although liquid mass spectrometry has high detection sensitivity and resolution, it is difficult for general laboratories to configure such an instrument

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