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Detection method of eccentric focusing-type laser-induced fluorescence detection device suitable for detection on capillary column

A laser-induced fluorescence and capillary column technology, which is applied in the research field of analytical instruments, can solve the problems of easy breaking, easy clogging, and difficult capillary processing, and achieves the effect of simple optical path structure and improved detection sensitivity.

Inactive Publication Date: 2013-08-14
ZHEJIANG UNIV
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Problems solved by technology

Although this method can effectively improve the detection sensitivity, the capillary with ultra-fine inner diameter is difficult to process, easy to clog, and relatively fragile, which limits the further application of this method in routine experiments and commercial instruments.

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  • Detection method of eccentric focusing-type laser-induced fluorescence detection device suitable for detection on capillary column
  • Detection method of eccentric focusing-type laser-induced fluorescence detection device suitable for detection on capillary column
  • Detection method of eccentric focusing-type laser-induced fluorescence detection device suitable for detection on capillary column

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Embodiment Construction

[0020] Referring to the accompanying drawings, the technical solutions of the present invention will be further described below through specific embodiments.

[0021] figure 1 It is a side view of the detection optical path of the eccentric focusing laser-induced fluorescence detection established according to the present invention for detection on a circular capillary column. Laser 1 is emitted by laser 2, purified by laser filter 3, reflected by dichroic mirror 4 placed horizontally at 45 degrees, and focused by objective lens 5, offset from the center of capillary 6 by a certain distance d in the horizontal direction Fluorescence is generated by excitation in the channel 8 of the circular capillary 7 . The inner diameter of the circular capillary ranges from 10-1000 microns, the outer diameter ranges from 50-5000 microns, and the shape of the cross-section of the capillary is a perfect circle or ellipse. The absolute value of the offset distance d is greater than 0 and le...

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Abstract

The invention relates to the field of analytical instrument research, and particularly relates to a detection method of a high-sensitivity laser-induced fluorescence detection device suitable for detection on a capillary column. The high-sensitivity laser-induced fluorescence detection device is characterized in that laser light is emitted from a laser, is filtered by a laser light filter, reflected by a dichroscope placed at a 45-degree angle relative to a horizontal plane and focused by an objective lens and finally enters a channel of a circular capillary tube from a position horizontally deviating from the center of the capillary tube by a certain distance so as to excite a component to be tested in the channel to generate fluorescence, and the fluorescence generated by excitation is collected by the objective lens, passes through the dichroscope, a focusing lens, a spatial filtering micropore and a fluorescence light filter respectively and finally enters a photoelectric detection device. The detection method not only is suitable for micro-column separation systems of capillary electrophoresis, capillary chromatography, capillary electrochromatography and the like, but also can be applied to analytic systems for flow injection analysis, sequence injection analysis, microfluidic liquid drop analysis, flow cytometry and the like based on circular capillary flow cells.

Description

technical field [0001] The field of the invention relates to the field of analytical instrument research, in particular to an eccentric focusing laser-induced fluorescence detection method and device suitable for detection on a capillary column. Background technique [0002] This patent relates to research on a highly sensitive fluorescence detection system suitable for detection on a capillary column. Laser induced fluorescence (LIF) is one of the most sensitive detection techniques in the field of chemical analysis. Compared with other detection techniques, laser-induced fluorescence detection has the following advantages: ultra-fine sample detection volume (picoliter to femtoliter), high sensitivity, high specificity, and high reproducibility. More importantly, combining laser-induced fluorescence detection systems with high-resolution separation techniques (such as capillary electrophoresis, capillary liquid chromatography), its application fields have expanded from ana...

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Application Information

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IPC IPC(8): G01N21/64
Inventor 祝莹方群陈念念
Owner ZHEJIANG UNIV
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