397 results about "Micro column" patented technology

A high-sensitivity, separation microcolumn assembly for a microgas chromatograph and the like is provided. The assembly has an ultra-low mass complete with integrated heaters. The assembly uses multiple zones for temperature control, and microstructures that permit very rapid heating and cooling of the microcolumn.

Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.

The invention discloses an integrated microfluidic sensing chip and a method for detecting microfluid. The integrated microfluidic sensing chip comprises a rigid polymer substrate, a polydimethylsiloxane circulating layer and a surface plasma resonance sensing sheet. Tested objects flow through a filter micro column of a filter passage of the polydimethylsiloxane circulating layer to be filtered and separated, and the tested objects which do not pass through the filter micro column enter a waste liquid passage and are discharged from a waste liquid outlet. The tested objects which pass through the filter micro column separation enter a sensing detection passage of the chip to react with specific biological molecules on the surface plasma resonance sensing sheet, and the surface plasma resonance sensing sheet carries out labeling-free and real-time quantitative detection for the biochemical reaction on the chip. The method integrates sample filtration and pretreatment, separation and labeling-free sensing detection, and has the characteristics of high integration degree, simple operation, high detection sensitivity, labeling prevention and little sample consumption. The integrated microfluidic sensing chip and the method are suitable for clinical diagnosis and analysis for body fluid containing complex components such as blood, urine, spit and the like.

The invention discloses a separate detection system and method of rare cells based on a centrifugal micro-fluidic technology. The system comprises a micro-fluidic chip similar to an optical disk, a centrifugal drive module and an optical detection module, wherein the micro-fluidic chip comprises a plurality of groups of micro-channels and micro-cavities arranged in a radiation manner; and the entire chip structure is composed of an elastic micro-column guide rail layer, a deformable film layer, a pipeline / cavity layer, a filtering membrane layer and a liquid waste collecting layer. The method comprises the steps of: firstly, leading a sample solution and immune modified microspheres into a liquid storage cavity through an injection port of the micro-fluidic chip in use, putting the sample solution and the immune modified microspheres on a centrifugal platform of the centrifugal drive module, assembling an elastic micro-column, and rotating at a low speed, so as to achieve fully mixing and reacting of the sample solution and immune modified microsphere liquid in the liquid storage cavity, separating by a high-speed rotary chip, and then dropwise adding a specifically recognized fluorescently-labeled antibody solution in each separate cell collection area, carrying out incubate reaction, adding a buffer solution and centrifuging, and finally identifying and analyzing through the optical detection module.

The invention relate to the field of medical detection, particularly relates to a micro-fluid cytometer and a manufacture method thereof, and solves the technical problems that the traditional cytometer at present is huge in size, high in price and particularly high in mutual infection rate. The micro-fluid cytometer mainly comprises a micro-fluid chip and a specially-made external detection component, wherein a sample channel, a focusing detection channel and a sheath flow liquid storage tank are arranged on the micro-fluid chip; a sample preprocessing region and a buffer region for carrying out concentrating processing on to-be-detected cell sap are integrated in the sample channel; and a micro-column array is arranged in the sample preprocessing region. The manufacture method of the micro-fluid cytometer comprises the following steps of: manufacturing micro-fluid channels, and manufacturing and bonding a cover plate of the micro-fluid chip. According to the micro-fluid cytometer and the manufacture method thereof, the products are integrally microminiaturized, the cost is low, the concentrating processing on the to-be-detected cell sap in the chip can be realized, the micro-fluid cytometer is disposable, each sample is measured at one time, and the problem of interaction effects among the samples is completely avoided.

Relating to the technical field of blood cell detection, the invention provides a blood cell rapid sorting device, which includes a microfluidic chip. The microfluidic chip comprises a pipeline for blood flow, and the center line of the pipeline is parallel to the fluid direction. Micro-column arrays are arranged in the microfluidic chip, and are in symmetrical arrangement at certain angle with the center line of the pipeline as the central axis. The invention also provides a manufacturing method of the blood cell rapid sorting device. According to the invention, by designing symmetrical micro-column arrays in the microfluidic chip, the micro-column arrays can separate particles of different sizes on the basis of the deterministic lateral displacement principle, and large cells can be screened out, for instance, circulating tumor cells or circulating endothelial cells larger than ordinary blood cells can be enriched and separated. The blood cell rapid sorting device has the advantages of rapid detection speed, simple operation, and low cost.

Field emitter as a source of electrons and method for making are provided. The emitter is formed by growth of a nitride compound of a group III element or alloys of group III elements on a substrate having a lattice mismatch with the thin film. The lattice mismatch causes columnar growth in the film. The micro columns have tips, thus forming an array of crystalline microtips of the low work function nitride material. The nitride compound is doped during growth. Gallium nitride grown on (111) silicon and doped with silicon produces a surface having low threshold electric field for emission and high current per unit area.

The invention relates to a fabrication method of the magnetic microstructure, which is characterized in that the invention comprises the fabrication of multilayer mould and the fabrication of the magnetic microstructure. The former is that the standard RCA cleaning process is used to clean silicon chips; multi layers of SU-8 photoresist are coated by rotating on the silicon chip surfaces and the multi layers of SU-8 mould are gotten after prebaking, photoetching, post baking and developing, and then the invention forms the magnetic microcolumn structure in the chip while molding dying the PDMS chip through directly filling the magnetic powders in the multilayer mould. Adopting the provided fabrication method can dispense with the electroplating process and fabricate the magnetic microstructure in the microfluid chip rapidly. The microstructure height is fabricated between micrometers less than ten and hundred of micrometers, which is determined by the photoresist layers of the rotating coating SU-8 and the magnetic microstructure is any one of the microcolumn, microline, microring or microcoil.

The invention discloses a circulation tumor cell automatic separation and purification micro-fluidic chip and a separation and purification method thereof. The circulation tumor cell automatic separation and purification micro-fluidic chip comprises a separation and purification chamber, a diversion column and micro columns, wherein a sample inlet, a first outlet from which target cells flow out, and a plurality of second outlets from which non-target cells flow out are formed in the separation and purification chamber; the separation and purification chamber is internally provided with two micro column arrays; each micro column array consists of a plurality of micro column rows; each micro column row comprises a plurality of micro columns; a gap is formed between every two adjacent micro columns of one micro column row; the gap forms a non-target cell channel; a gap is formed between two micro column arrays; the gap forms a target cell channel from which the target cells pass; the width of the target cell channel is greater than that of the non-target cell channel; an acute angle is formed between the micro column rows and the target cell channel; and the acute angle faces to the sample inlet. The circulation tumor cell automatic separation and purification micro-fluidic chip is high in separation and purification degree.

A device and methods for performing biological or chemical analysis is provided. The device includes an array of three-dimensional microcolumns projecting away from a support plate. Each microcolumn has a relatively planar, first surface remote from the support plate. An array of multiple, different biological materials may be attached to the first surface. The device, when used in combination with existent micro-titer well plates, can improve efficiency of binding assays using microarrays for high-throughput capacity.

The invention provides a mass transfer method of micro devices. The method comprises steps of: 1) capturing a micro device by using an entire photosensitive material; 2) making the photosensitive material into a trapezoidal structure and a supporting micro column by using the micro device as a photolithographic mask plate; 3) breaking the supporting micro column by using mechanical force to achieve the mass transfer of the micro devices. The entire photosensitive material is used to catch the micro devices so as to avoid the insufficient alignment accuracy of catching the micro devices. The method uses the micro device as the photolithographic mask plate and makes the photosensitive material into the trapezoidal structure and the supporting micro column so as to be beneficial to the stability of the micro device and the simplicity of subsequent separation. The mass transfer of the micro devices can be achieved just by breaking the supporting micro column with mechanical force. The method is simple in process and can reduce process cost.

The invention discloses a three dimensional structure micro zinc-nickel battery applying to microsystems and a preparation method thereof, which belongs to the micro battery fabrication technique field. The structure of the micro zinc-nickel battery is as follows: preparing gold combdent two dimensional plane structural current collector on the silicon substrate surface by adopting micro processing technique, preparing three dimensional stereo space structural nickel microcolumn anode and zinc microcolumn cathode on the current collector surface, depositing nickel oxide active electrode substance on the nickel microcolumn anode surface, filling and covering a layer of gel-like solid electrolyte between the anode and cathode of the three dimensional stereo space structure, and covering a layer of polyimide material on the battery surface for finishing encapsulation of the micro zinc-nickel battery. The fabrication technique based on microsystems technique is characterized of simple technique, being suitable for batch production and the like. The micro zinc-nickel battery is characterized of small volume, high storage energy, stable performance and the like, which applies widely to micro robot electronic intelligent system, chemical sensor, battlefield friend or foe identification equipment, distributed battlefield sensor and the like.

The present invention discloses an apparatus for forming and screening a large-scale single-layer two-dimensional liquid droplet array, and a use method thereof. The apparatus comprises a micro-column array chip, a scraping plate for auxiliary spreading of a liquid droplet, a probe for controlling the liquid droplet, a three-dimensional translation table and other components, wherein a micro groove unit for trapping the single liquid droplet is formed by using the gap between micro-columns, and the liquid droplet is spread in the micro groove unit in a single-layer and single manner through gravity effect and capillary effect or through an auxiliary means so as to form the single-layer liquid droplet array. With the apparatus of the present invention, the accurate positioning and detection on the liquid droplet, the sucking on the target liquid droplet and the subsequent treatments can be performed. According to the present invention, the apparatus has characteristics of simple structure and easy operation; and the method has advantages of fast liquid droplet spreading, high flux, high liquid droplet trapping efficiency, liquid droplet position fixing and the like, and is suitable for single cell analysis and screening, single molecule analysis and screening, high-throughput gene screening, protein directed evolution, antibody screening, microbial research, drug screening, and other fields.

The invention provides a leadless piezoelectric ceramics / polymer 1-3 structure composite material and processing method thereof, belonging to functional ceramic and manufacture technique field. The composite material is composed of (Na, K)NbO3 leadless piezoelectric ceramics and epoxy resin. The ceramics column is dispersed in the epoxy resin matrix and the piezoelectric ceramic micro column arrays obtained by mechanical treatment are arranged in the matrix of polymer wherein the cross sectional width of the piezoelectric ceramic micro column is 40-100 um, the aspect ratio is 3-10. The processed micro column arrays with cross sectional width of 40-100 um are arranged in the matrix of polymer. During cutting the piezoelectric ceramic, the resin is filled after first time parallelism cutting, so that the obdurability of the processed sample and integrity of micro column column is greatly enhanced, therefore the fine processing dimension of the piezoelectric ceramic micro column is increased by one order of magnitude. The 1-3 structure order composite piezoelectric material with fine structure can be used in high frequency medical ultrasound imaging technique.

A micro column electron beam apparatus having a reduced number of interconnections is provided. The micro column electron beam apparatus includes: a low temperature co-fired ceramic (LTCC) substrate; a plurality of deflector electrodes attached to a predetermined top portion of the LTCC substrate; a pad electrode placed at a top edge of the LTCC substrate and transmitting an external signal to the deflector electrodes; and a connection unit placed in the LTCC substrate and electrically connecting the deflector electrode and the pad electrode.

Provided is an electron beam lens for a micro-column electron beam apparatus and a method of manufacturing the same. A photosensitive glass substrate is used as a base isolation substrate and a thin metal film is grown by a plating method. Holes through which electron beam passes are formed by a lift off method after forming a resist pattern shaped as a hole on a seed metal layer and plating the thin metal film.

A microbial fuel cell includes a bio-compatible body having a micro-pillar structure defining an anode compartment adapted to contain a catalyst that metabolizes glucose to generate electrons and protons. A nano-porous membrane prevents loss of the catalyst from the anode compartment, while providing fluid access for ingress of glucose fuel and egress of waste.

Improved micro-columns and methods for producing micro-columns particularly suitable for use in gas chromatographs are disclosed. In particular, following deposition of the stationary phase coating, the micro-columns are subjected to a postcoating treatment with a molecule that binds to the active sites in the stationary phase micro-column thereby eliminating or reducing loss of gas chromatograph performance associated with those active sites. The postcoating treatment molecule binds to the same active sites as the analytes of interest.

The invention discloses a high sensitive biochemical sensor based on a resonance oscillation type micro cantilever beam structure. The biochemical sensor comprises a resonance oscillation chamber, a cantilever beam and a Whist bridge type detection circuit, wherein a micro column structure (3), a liquid storage tank (4) and a liquid leakage hole (5) through which biochemical solution flows are etched at the free end of the cantilever beam; the support end of the cantilever beam is connected with the Whist bridge type detection circuit; the Whist bridge type detection circuit is composed four U-shaped piezoresistance bars (1), three input electrodes (8, 9, 10) and two output electrodes (7, 11); and the four piezoresistance bars (1) are connected with the input electrodes (8, 9, 10) and the output electrodes (7, 11) through a signal transmission line (2). With the adoption of the biochemical sensor, micro biochemical molecules can be sensed with a high sensitivity by detecting the frequency change after an object to be tested is absorbed; biochemical molecules with micromass can be detected; and the biochemical sensor can be widely used in engineering fields such as medicine and chemistry.

The invention relates to a manufacturing method of a complex drag reduction coating with a flexible wall and an imitation shark skin micro-groove, and the method comprises the following three major steps: a step 1) preparing a female mold plate A; a step 2) preparing a female mold plate B; and a step 3) manufacturing the complex drag reduction coating by micro-molding and stamping. By adopting the molding and stamping reproduction method, the skin morphology of a shark and a micro-column array can be simultaneously reproduced, the obtained complex drag reduction coating simultaneously has the flexible wall for drag reduction and the shark micro-groove, the complex drag reduction efficiency is high, and the practicality and the operability are stronger; and the complex drag reduction coating prepared by the method is simple in process and low in cost, and has the advantages of better practical value and broad application prospects in the technical field of biological bionics.

The invention discloses a method for preparing an aflatoxin B1 aptamer affinity column and belongs to the field of affinity chromatography and mycotoxin analysis. The method comprises the following steps: adopting epoxy activated agarose microspheres FF as a solid phase carrier; after the solid phase carrier is coupled with an amino-modified aflatoxin B1 aptamer, closing extra active sites in the carrier, and feeding into micro columns. The prepared aptamer affinity column can be specially combined with aflatoxin B1, the adding standard recovery is 70-110, and the aptamer affinity column can be repeatedly used for at least five times.

The invention provides a controllable design method for a stable superhydrophobic surface of a microcolumn structure. A critical height criterion for a liquid drop to form a stable Cassie state is proposed from the perspective of a minimum energy principle and can serve as a theoretical criterion for the preparation of a stable superhydrophobic microstructure. The critical height is calculated to perform theoretical prediction on whether the stable Cassie state can be formed or not, so that the controllable design of the stable superhydrophobic surface is realized. The surface soaking performance of the designed microcolumn structure is verified through Fluent software numerical simulation of static sessile drops and dynamic residence of the designed microcolumn structure. The superhydrophobic surface design method for the periodically arranged microcolumn structure, proposed by the invention, considers the influences of the microcolumn height, the microcolumn spacing, the microcolumn bottom surface edge length and an intrinsic contact angle on the surface hydrophobic performance of the microcolumn structure at the same time, and relates to the fields of superhydrophobic design, material simulation and the like; a design process is convenient and quick; and the design method is easy to master.

The invention relates to a microarray-type structure chip adopting micro-flow pipes and taking advantage of diameter difference of different types of cells, and micro column arrays with different intervals and different sizes are etched in the micro-flow pipes. The chip structure is formed by up-down bonding of a silicon Si material negative-film substrate and a soda-lime glass upper cover. According to the different intervals of the column micro structure, the microarray-type structure chip can be divided into two series: variable interval and fixed interval; and according to the different sizes of the column micro structure, the microarray-type structure chip can be divided into a plurality of types: ranging from 2-30 [mu] m. The microarray-type structure chip is characterized in that: when a liquid fluid containing the different types of cells flows through the chip micro structure, and passes through the column microarrays with different intervals and diameters, different types (different sizes) of cells can be captured by different micro column array structures, and retained on the different micro column arrays with different micro structures in the pipes, and the captured results can be observed and analyzed by used of a microscope. The microarray-type structure chip has the characteristics of micro volume, flexible and customizable structure, no moving parts and the like, and can be widely applied to capture and analysis and other fields of various cancer cells in the blood, and comprehensive analysis and measuring and calculating determine of the cell type and quantity in the blood of patients can be conducted through use of different kinds of chips.

The invention relates to a preparation method for anti-ice surfaces of bionic coupling water collecting aluminum alloys; the preparation method is enlightened by water collecting structures at the back parts of desert beetles; and the surfaces of the bionic coupling water collecting aluminum alloys are prepared by adopting a method of coupling hydrophobic areas with hydrophilic areas. In the method, firstly, a laser ablation machining process is adopted to prepare micro-column array structures with a certain depths on the surfaces of aluminum alloys; prepared PDMS and super-hydrophobic kaolinparticle mixtures are rotationally coated in the micro-column array structures, and are placed in a drying box of 100 DEG C for heating by 120 min; and finally, the laser ablation is performed on thesurfaces again to prepare the anti-ice surfaces of the bionic coupling water collecting aluminum alloys. The preparation method is simple in operation, controllable in experimental parameter and low in cost; the method can be used for preparing the anti-ice surfaces of the bionic coupling water collecting aluminum alloys; and the surfaces achieve better ice resistance, excellent mechanical stability and aging resistance.

Provided is a wafer-scale microcolumn array using a low temperature co-fired ceramic (LTCC) substrate. The microcolumn array includes a LTCC substrate having wirings and wafer-scale beam deflector arrays, which are attached to at least one side of the LTCC substrate and has an array of deflection devices deflecting electron beams. The wafer-scale microcolumn array using the LTCC substrate makes it possible to significantly increase the throughput of semiconductor wafers, simplify its manufacturing process, and lower its production cost.

The invention provides a microfluidic chip and a circulating tumor cell capture method using the same. The microfluidic chip comprises a separating cavity, a micro-pillar array and an external magnetic field assembly, wherein a sample injection opening is formed in the middle of a first side of the separating cavity; a to-be-detected particle enrichment part and a sample outflow opening are respectively arranged at the upper part and the lower part of a second side; the micro-pillar array is arranged in the separating cavity and between the sample injection opening and the sample outflow opening; the external magnetic field assembly is used for forming a magnetic field inside the separating cavity from the bottom up; among samples injected from the sample injection opening, to-be-detected particles of which the surfaces are connected with magnetic beads move upwards under the application force of the magnetic field, and are enriched at the to-be-detected particle enrichment part or attached onto the top wall of the separating cavity, and to-be-detected particles of which the surfaces are not connected with magnetic beads are intercepted and captured by the micro-pillar array; besides the to-be-detected particles, other components in the samples penetrate through the micro-pillar array under the gravity action. According to the method, two capture modes are combined, so that high capture rate and high throughput are realized, and high-purity circulating tumor cells are captured.

The invention relates to a pre-plating layer auxiliary preparation method of an X-ray flash conversion screen with a micro-column structure CsI (Tl) and an application of the conversion screen. The method comprises the following steps of: preparing a pre-plating layer by a thermal evaporation technology, realizing the pre-plating layer with uniformly distributed island grain structure and effectively controlling the grain distance through the adjustment of the thickness of the pre-plating layer, an annealing technology and the like, then preparing subsequent flash thin film on a substrate plated with the pre-plating layer by using CsI (Tl) powder as a raw material by the thermal evaporation, and realizing the effective controls on micro-column morphology, uniformity, line width, preferred orientation of a crystal face of the conversion screen and the like. The flash micro-column which is nearly vertical to a screen surface and has good crystallization property can guide flare light to spread along the direction of the micro-column, so that the spatial resolution of an X-ray image device is improved, and requirements on the high spatial resolution and high detection efficiency are met. The coupling of the X-ray flash conversion screen with the micro-column structure CsI (Tl) and a photoelectric detector can be applied to a digital X-ray imaging of high resolution. The pre-plating layer auxiliary preparation method of an X-ray flash conversion screen with the micro-column structure CsI (Tl) is suitable for industrial production and is high in popularization and application values.

To obtain a microcolumnar structured material having a desired material. The columnar structured material includes columnar members 15 obtained by introducing a filler into columnar holes formed in a porous material. The porous material has the columnar holes 14 formed by removing columnar substances from a structured material in which the columnar substances 12 containing a first component are dispersed in a matrix member 13 containing a second component capable of forming a eutectic with the first component. The matrix member 13 may be removed. In the columnar structured material, the filler is a conductive material, and an electrode can be structured by electrically connecting the conductive materials in at least a part of a plurality of holes to a conductor.