899 results about "Bio molecules" patented technology

The present invention relates composite core / shell nanoparticles and a two-step method for their preparation. The present invention further relates to biomolecule-core / shell nanoparticle conjugates and methods for their preparation. The invention also relates to methods of detection of biomolecules comprising the biomolecule or specific binding substance-core / shell nanoparticle conjugates.

Free-standing microfluidic channels are used to both transport and analyze molecules of interest. In a biochemical context, such molecules may be polypeptides, nucleic acids, or other biomolecules. The free-standing channels provide a real-time readout of concentration without the need for labeling with reporter molecules. The channels can also measure enthalpy values and equilibrium constants by detecting heat released from or absorbed by the sample.

Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.

The present disclosure relates to methods of rapidly and efficiently searching biologically-related data space to identify a population set maximally diverse and optimized for sets of desired properties. More specifically, the disclosure provides methods of identifying a diverse, evolutionary separated bio-molecules with desired properties from complex bio-molecule libraries. The disclosure additionally provides digital systems and software for performing these methods.

The present invention relates composite core / shell nanoparticles and a two-step method for their preparation. The present invention further relates to biomolecule-core / shell nanoparticle conjugates and methods for their preparation. The invention also relates to methods of detection of biomolecules comprising the biomolecule or specific binding substance-core / shell nanoparticle conjugates.

The present invention relates to screening methods, compositions, and kits for detecting for the presence or absence of one or more target analytes, e.g. biomolecules, in a sample. In particular, the present invention relates to a method that utilizes reporter oligonucleotides as biochemical barcodes for detecting multiple protein structures or other target analytes in a solution.

An integrated lab-on-a-chip device for carrying out an assay to detect the presence of a biological molecule in a fluid sample, the device comprising: (a) an inlet for a fluid sample; (b) one or more reaction sites each in fluid communication with the inlet; (c) one or more reagent reservoir systems each containing reagents required for an assay to detect a biological molecule, the reagents being arranged sequentially in each reservoir system in the order in which they are required for the assay and separated from one another by a fluid.

A high speed, highly sensitive optical sensing platform and a method for detecting and / or measuring characteristics of a substance in a measurement sample are disclosed. The platform includes at least one pair of optical paths formed in a waveguide, a light source for injecting optical beams along the optical paths, a light modulator for enabling the excitement of a transverse electric and a transverse magnetic guide modes, and a phase detector for detecting phase differences between the beams propagating along the optical paths. One of the optical paths has a target analyte of unknown concentration with a measurement sample bound to its upper surface, while the second optical path has a reference sample containing a known concentration of the target analyte bound to its upper surface. A guided mode modulator causes an optical beam to propagate through the waveguide sequentially as two polarized modes. The highly sensitive platform is especially useful for directly detecting and / or measuring very small numbers of small molecules, bio-molecules, microorganisms in a liquid or gaseous test sample.

Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The power of Nature and the flexibility of organic synthesis are combined in nucleic acid-templated synthesis. The present invention provides a variety of template architectures for performing nucleic acid-templated synthesis, methods for increasing the selectivity of nucleic acid-templated reactions, methods for performing stereoselective nucleic acid-templated reactions, methods of selecting for reaction products resulting from nucleic acid-templated synthesis, and methods of identifying new chemical reactions based on nucleic acid-templated synthesis.

Methods are provided for tagging, characterizing and sorting double-stranded biomolecules while maintaining the integrity of the biomolecules.

The present invention provides devices, methods and systems to selectively detect the binding of a molecular species to a biomolecule. In its olfactory sensing application, the hybrid sensor arrays of the present invention provide a high dimensional signature of odorants present that is also readily reversible, together enabling the identification and localization of a source analyte in the presence of the background odorant landscape inherent in a real-world setting.

The invention provides devices and methods using assemblies of shaped inserts coated with diverse binder materials optionally positioned inside conformal housings. The devices are capable of immobilizing target analytes of interest (e.g., specific or groups of biomolecules) and are optionally usable as single units, linear strips or array formats. The coatings on the inserts and, optionally also on the inside walls of the housings, create variable gaps with narrow fluid paths resulting in enhanced diffusion and absorption of target analytes from transiting fluid samples while exhibiting minimal resistance to flow or back pressure commonly seen in conventional plug-type packings. The devices are intended for sample preparations requiring: filtering, enriching, separating, purifying of target analytes by selective absorption / elution as part of desalting, buffer exchange and / or enrichment applications in analyses by mass spectrometry and / or electrophoresis; purifying biomolecules; culturing cells; analytical separation processes, and general chemical, biological and / or biochemical separations in manual or automated systems.

The invention is drawn to novel nanostructures comprising hollow nanospheres and nanotubes for use as chemical sensors, conduits for fluids, and electronic conductors. The nanostructures can be used in microfluidic devices, for transporting fluids between devices and structures in analytical devices, for conducting electrical currents between devices and structure in analytical devices, and for conducting electrical currents between biological molecules and electronic devices, such as bio-microchips.

The invention relates to techniques for measuring and comparing relative molecular flux rates of different biological molecules by administering isotope-labeled water to one or more tissues or individuals and comparing the molecular flux rates of two or more biological molecules, including biological molecules in different chemical classes. The methods find use in several applications including diagnosing, prognosing, or monitoring a disease, disorder, or condition, the in vivo high-throughput screening of chemical entities and biological factors for therapeutic effects in various disease models, and the in vivo high-throughput screening of chemical entities and biological factors for toxic effects.

Polymeric coatings that include coupled biomolecules are formed on the surface of articles, such as medical devices. A coated layer that includes a synthetic polymer including a reactive group is formed on a surface and then a biomolecule is attached to the reactive group. The synthetic polymer can be an acrylate polymer or an amine-containing polymer having pendent photoreactive groups.

Arrays are provided for multiplexed evanescent scanning by allowing for high-contrast Surface Plasmon Resonance images thereof. The arrays target features are typically biopolymeric in nature, though they may be any sort of chemical or ligand. The type of scanning is such that there is no need for probe labeling. As no labeling is required, a broader range of applications than otherwise possible is facilitated. In the subject arrays, target features are set upon a noble metal film deposited on a substrate. Interfeature areas are adapted to trap, divert and / or bleed-away light so that light directed through the substrate will not be reflected by those areas and interfere with evanescent scanning of the reflective areas upon which intended target features are provided. Geometric and materials-based light attenuating features are contemplated. Arrays as described, hardware and software as required for reading such arrays, and associated methodology are covered.