The invention discloses a two-dimensional flow velocity field measurement method and device of an interlaced scanning CCD (charge coupled device). Trace particles are implanted into a to-be-measured flowing object (gas or liquid) and can move together with fluid due to viscidity. For the measurement device, a continuously light-emitting gas laser (such as He-Ne laser) is used as a light source, and a common interlaced scanning CCD sensor is used as an image detector. A one-frame flowing image including the trace particles is captured transiently, then the flowing image is processed by means of filtering and binaryzation, the interlacedly scanned one-frame image is separated into two images according to odd-even lines, a cross-correlation technology is adopted to perform computation processing, and accordingly a two-dimensional flow velocity field can be obtained. PIV (particle image velocimetry) full-field velocity measurement can be achieved without a high-power laser, a high-resolution CCD and a complex synchronizer; and the two-dimensional flow velocity field measurement method and device is simple in equipment, low in cost and applicable to flow field measurement on different occasions.