The invention provides an establishing method for a rabbit sinus node chronic injury model. The method comprises the following steps of: after anaesthetizing a rabbit, fixing the back of the rabbit on a rabbit table, and recording body surface electrocardiogram; shaving the skin at a colpus area, longitudinally opening the chest along a third rib at the right side of sternum, separating epidermis and muscular tissues layer by layer, cutting off the third rib of the right side, and opening pericardium through mediastinum to expose auricula dextra; placing an electrode connected with an electrocardiogram V1 lead to a near sinus node area of the rabbit and slowly moving, when the electrocardiogram displays electrograph characteristics of the sinus node area, applying 20% formaldehyde solution to the electrode area, when the heart rate of the rabbit is decreased to 50-70% of original heart rate, intravenously injecting 2mg of atropine sulphate until the heart rate stops rising, and stopping applying formaldehyde solution; and cleaning the chest, closing the chest layer-by-layer, and feeding for later use. The invention also provides a sinus node targeted injection penetration electrode for implementing the method. Compared with the prior art, the establishing method is high in modeling success rate, stable in model and good in feasibility and repeatability..