The invention provides a noninvasive detection kit for
nasopharyngeal carcinoma susceptibility genes. The kit comprises a specific primer,
a DNA sequencing primer, a PCR (
Polymerase Chain Reaction) component, a PCR product purification component,
a DNA sequencing reaction component and the like, wherein the specific primer is used for detecting the polymorphic site genotypes of four mononucleotides of the Rsall polymorphism on a CYP2E1
gene, the polymorphism of a G252 site on a TNF (
Tumor Necrosis Factor)-
alpha gene, the deletion (Present / Null) of a GSTM (Giant Solide Turner Of The
Mediastinum) 1
gene and the deletion (Present / Null) of a GSTT (Gross
Saponin From
Tribulus Terrestris) 1
gene. According to the kit, the
risk level of
nasopharyngeal carcinoma of a subject is evaluated according to the genotypes of two mononucleotide polymorphic sites closely related to the
nasopharyngeal carcinoma, and then, according to the gene detection results of each subject, the subjects are guided from the gene level to specifically prevent the nasopharyngeal
carcinoma so as to reduce the morbidity risk rate of the nasopharyngeal
carcinoma. The sampling method of the kit adopts oral
mucosal cell sampling which is painless and non-invasive, and cross-infections are avoided. The sequencing detection result of the kit is accurate and reliable, and is easy to popularize because expensive import special instruments are not needed to be purchased.