Oxytropis Ehrig cinerea FEL2-OG and separation method and application thereof
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A technology of FEL2-OG and spore bacteria, applied in the field of microorganisms, can solve the problems of lack of swainsonine sources and high price
Inactive Publication Date: 2011-11-09
NORTHWEST A & F UNIV
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Swainsonine has good prospects for developing new anti-tumor drugs, but the source of swainsonine is very scarce and expensive, which is far from meeting the needs of people's research and clinical treatment
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Embodiment 1
[0028] Example 1: Isolation of Ehrigia spinosa FEL2-OG
[0029] 1) Wash the whole plant Oxytropis glacialis grown on the alpine meadow in Ngari region of Tibet with deionized water to remove the dust on the surface of Oxytropis glacialis, and separate the leaves, stems and seeds of the plant with sterilized tweezers, and then separate the leaves, The stems and seeds were wrapped with gauze for surface disinfection.
[0030] 2) Use sterilized filter paper to absorb the water on the surface-sterilized plant tissue, then use sterilized scissors to cut leaves, stems, seeds, etc. into 3mm tissue pieces, and then use sterilized forceps Each tissue block was inoculated on the surface of water agar medium, and cultured in an incubator at 18°C.
[0031] 3) When the mycelium grows from around the tissue block, pick the mycelium at the edge of the colony and inoculate it on the surface of the enrichment medium, and place it in an incubator at 18°C for enrichment culture; collect the f...
Embodiment 2
[0032] Example 2: Identification of Ehrigia spinosa FEL2-OG
[0033] 1. Morphological features
[0034] The colony of the FEL2-OG strain was round, white at first, and gradually changed to light yellow, yellowish brown, dark brown with the prolongation of the culture time, with a slight protrusion in the center ( figure 1 ). The mycelium has a transverse septum without a mediastinum, and some old hyphae give birth to conidiophores, which have branches. Conidia and conidiophores with septum, without mediastinum, the septum of conidia is thicker, the number of septa varies from 1 to 3, the spores are rod-shaped, nearly cylindrical, and the size of conidia is 20-70 μm ×5~10μm( figure 2 ).
[0035] 2) Characteristics of culture
[0036] The strain grows very slowly on PDA, PCA, Czapek and other media, and its growth rate is 0.50, 0.58 and 0.37mm / d respectively. The optimum growth temperature is 18°C, and the growth is inhibited when the temperature is higher than 25°C.
[003...
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Abstract
The invention discloses an oxytropis Ehrig cinerea FEL2-OG which is preserved in China type culture collection center on October 18, 2008, and the preservation code is CCTCC NO: M 208142. Bacterial colony of bacterial strain is in a circular shape, is white in the beginning, and gradually changes to primrose yellow, yellow brown and black brown, and the centre of the bacterial colony slightly protrudes. Mycelium has transverse septa but has no mediastinums. Conidiophores peduncles grow on part of the aging hypha h, and the conidiophores have braches. Conidiophores and the conidiophores peduncles have transverse septa but have no mediastinums, the transverse septa film of the conidiophores is thicker, and the number of the transverse septa is 1 to 3; spores are in long rod shapes and are similar to column shapes, and the size of the conidiophores is 20 to 70 microns*5 to 10 microns. The bacterial strain is obtained from leaves, stems and seeds of oxytropis glacialis through manual separation and filtration, and has the function of synthesizing main toxin, that is swainsonine (Swainsonine, SW) of locoweed.
Description
technical field [0001] The invention belongs to the technical field of microbes, and relates to a new type of Ehrigia spp. (Embellisaoxytropis), in particular to a type of Ehrysotropis capable of synthesizing the main toxin of madweed, swainsonine (Swainsonine, SW). FEL2-OG also relates to the isolation method and application of the bacterium. Background technique [0002] Locoweed is the general term for the poisonous plants of the genus Oxytropis and Astragalus of the leguminous family, and it is also the most serious poisonous weed that endangers grassland animal husbandry in the world. In my country, locoweeds are widely distributed in the vast grasslands of Northwest, North and Southwest China, with an area of damage reaching 11 million ha 2 , accounting for 2.8% of the country's grassland area. Oxytropis glacialis is a kind of the locoweed genus Oxytropis in my country, which is mainly distributed in the Ngari area of Tibet, China; Oxytropis glacialis mainly harm...
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