Oxytropis ehrig verticillium FEL5-AS1 of synthesized swainsonine and application thereof
A technology of FEL5-AS1 and swainsonine, applied in the field of microorganisms, can solve the problems of high price and lack of swainsonine sources
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Embodiment 1
[0018] Embodiment 1: Isolation and screening of fungus Ehrigia spinosa FEL5-AS1
[0019] 1) Wash the whole plant of Astragalus membranaceus grown on the Shigatse grassland in Tibet with deionized water to remove the dust on the surface of Astragalus membranaceus, and use sterilized tweezers to separate the leaves, stems and seeds of the plant, and then separate the leaves, stems and seeds Wrap them with gauze respectively for surface disinfection.
[0020] 2) Use sterilized filter paper to absorb the water on the surface-sterilized plant tissue, and then use sterilized scissors to cut leaves, stems, seeds, etc. into 3mm 2 Then inoculate each tissue piece on the surface of water agar medium with sterilized tweezers, and culture it in an incubator at 18°C.
[0021] 3) When the mycelium grows from around the tissue block, pick the mycelium at the edge of the colony and inoculate it on the surface of the enrichment medium, and place it in an incubator at 18°C for enrichment cul...
Embodiment 2
[0022] Embodiment 2: the identification of fungus Ehrigia spinosa FEL5-AS1
[0023] 1. Morphological features
[0024] The colony of the FEL5-AS1 strain was round, white at first, and gradually changed to light yellow, brown, dark brown with the prolongation of the culture time, with a slight protrusion in the center ( figure 1 ). The mycelium has a transverse septum and no mediastinum. Some old hyphae produce conidiophores with few branches. The spore-forming peduncles are bent knee-like and have obvious spore scars. Conidia and conidiophores with septum, without mediastinum, the septum of conidia is thicker, the number of septa varies from 1 to 4, the spores are rod-shaped, nearly cylindrical, and the size of conidia is 20-90 μm ×5~10μm( figure 2 ).
[0025] 2) Characteristics of culture
[0026] The strain grows very slowly on PDA, PCA, Czapek and other media, and its growth rate is 0.60, 0.51 and 0.48mm / d respectively. The optimum growth temperature is 18°C, and the ...
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