Pseudomonas aeruginosa for preventing and treating vertieillium wilt in crops and application of pseudomonas aeruginosa
A technology for Pseudomonas aeruginosa and crops, applied in the direction of chemicals for biological control, methods based on microorganisms, applications, etc., can solve the problems of large differences in antagonism effects, and achieve high-efficiency antagonism effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] Example 1 strain isolation and screening
[0027] The tested strains were isolated from the cotton experimental field of Zhejiang University Huajiachi Campus in Hangzhou City, Zhejiang Province.
[0028] Screening of biocontrol bacteria in soil by double-layer plate method. Put the Verticillium dahliae cake that has been cultured for 4-5 days in sterile water, and prepare a concentration of 10 6 per mL of spore suspension. Take an appropriate amount (1g) of soil sample to prepare a soil suspension in sterile water, and serially dilute to 10 -2 -10 -3 . Double-layer plate method: evenly coat the prepared spore suspension of Verticillium dahliae on PDA, grow at 28°C for 48 hours, pour LB on the upper layer of PDA, and evenly coat the soil suspension. Place the plate under dark conditions at 28°C for culture. After the colonies grow out, select the colonies with inhibition zones for purification and culture, and then carry out re-screening.
[0029] A total of 1 stra...
Embodiment 2
[0036] The identification of embodiment 2 bacterial strains
[0037] Morphological identification
[0038] The edge of the colony of the strain on LB medium was irregular, and a transparent halo was formed around the colony. Stain test determined Gram-negative bacteria. Under the scanning electron microscope, it is rod-shaped bacteria with a size of 0.7-0.9μm×1.8-3μm( figure 1 B).
[0039] 16SrDNA sequence analysis
[0040] The strain was inoculated in NA culture medium (yeast powder 1g / L, beef extract 3g / L, peptone 5g / L, sucrose 10g / L, pH=7.0), and after culturing at 30°C and 180rpm for 120 hours, DNA extraction was performed; 16SrDNA general primers were used for PCR amplification to obtain an electrophoretic band of about 877bp, which was recovered and sequenced by Sangon Bioengineering Co., Ltd. (Shanghai). The determined 16SrDNA sequence was compared with the sequence in the GenBank database to determine its taxonomic status.
[0041]16SrDNA sequencing, after compar...
Embodiment 3
[0049] The fermentation culture of embodiment 3 bacterial strain KK9a and the detection of antagonistic component
[0050] The culture medium can be LB liquid medium, its formula is: tryptone 10g / L, yeast extract powder 5g / L, sodium chloride 10g / L, adjust the pH to 7.0-7.2.
[0051] 50L automatic fermenter culture: inoculate Pseudomonas aeruginosa in LB culture medium and cultivate to culture medium OD 600 0.6 to 0.8, to obtain the seed solution; inoculate the seed solution into a 50L fermenter, the expansion culture temperature should be between 35 and 37°C, the culture time should be between 48 and 60h, the aeration ratio should be 0.4 to 2:1, and the rotation speed 300~600rpm, pH should be between 7~7.5. The fermentation medium is: tryptone 10g / L, sucrose 3g / L, sodium chloride 2.5g / L, adjust the pH to 7.0-7.2, and sterilize at 121°C for 30 minutes. The final bacterial concentration can usually reach 1×10 10 ~1×10 11 CFU / mL.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com