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Pseudomonas chlororaphis for preventing and treating crop fusarium disease and applications thereof

A technology of Pseudomonas chloropinus and biocontrol agents, applied in the direction of chemicals for biological control, methods based on microorganisms, applications, etc., to achieve high-efficiency antagonism and good antagonism effects

Active Publication Date: 2015-04-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although many kinds of Pseudomonas have been developed for the preparation of microbial fungicides, due to the diversity of Pseudomonas populations, that is, strains, different Pseudomonas and even different strains of the same Pseudomonas have different Antibacterial spectrum, antibacterial activity and antibacterial characteristics, and the screening of highly effective Pseudomonas biocontrol agents with different targets are still the subject of scientific research work in various countries for a relatively long period of time now and in the future

Method used

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  • Pseudomonas chlororaphis for preventing and treating crop fusarium disease and applications thereof
  • Pseudomonas chlororaphis for preventing and treating crop fusarium disease and applications thereof
  • Pseudomonas chlororaphis for preventing and treating crop fusarium disease and applications thereof

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Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1 The preparation of the biocontrol agent containing Pcho01 bacterial strain

[0039] Acquisition of 1Pcho01 strain

[0040] Pseudomonas chloropinus Pcho01 was isolated from tomato rhizosphere in Xiaoshan City, Zhejiang Province. The bacterium is Gram-negative bacteria, no spores, unipolar flagella, able to move. After culturing on KMB medium for 24 hours, 1.2mm colonies can be formed. The colonies can produce orange pigment, round shape, raised surface, smooth, viscous, easy to pick up, and neat edges (see figure 1 ). Its 16S rDNA sequence is shown as SEQ ID No.1, and the comparison result at NCBI shows that it is Pseudomonas choloraphis.

[0041] 2 Preparation of biocontrol agent

[0042] 1) Inoculate the Pcho01 strain into the LB culture medium, culture at 30°C and shake at 180rpm for 12-16 hours, then take samples in the ultra-clean workbench to measure the OD value at 600nm. zeroing;

[0043] The formula of LB culture medium is: sodium chloride 10g / L...

Embodiment 2

[0046] The antagonistic activity of embodiment 2 Pcho01 bacterial strains to Fusarium graminearum

[0047] Determination of plate antagonistic activity of 1Pcho01 strain against Fusarium graminearum

[0048] Using the confrontation culture method, inoculate Fusarium graminearum PH-1 (model bacteria) stored at 4°C on a PDA plate for activation. Beat into a round bacterium block with a diameter of 6mm. Inoculate the mycelium block on the center of the WA plate (WA medium / L: peptone 5g, glucose 10g, gravy extract 3g, sodium chloride 5g, agar 20g, pH=7.0), and place them around 25mm away from the center respectively. Pcho01 was inoculated, and water was used as the control group. Cultivate at 25°C, and record the size of the inhibition zone after the mycelia cover the plate.

[0049] The activity of Pcho01 against Fusarium graminearum was evaluated according to the size of the inhibition zone: 0mm, no inhibition; 5mm, strong inhibition effect. The inhibition rate is calculate...

Embodiment 3

[0071] The control activity of embodiment 3 Pcho01 bacterial strains to wheat head blight

[0072] 1 Evaluation of the effect of Pcho01 strain on controlling wheat head blight in light incubator

[0073] Collect wheat ears at the flowering stage, and spray inoculate 10 8 CFU / mL (containing 0.05% Tween20) of the Pcho01 strain until the water droplets flow (about 5mL per panicle), and then placed in the light incubator, 25 ℃ moisturizing for 24h. The next day, Fusarium graminearum PH-1 spore suspension (10 5 per mL, containing 0.05% Tween20) spray inoculated to wheat ears (spray until small water droplets are formed, about 4 mL per ear), and then placed in a light incubator at 25°C and 90% humidity to observe the disease.

[0074] Four treatment groups were set up in the experiment: Pcho01, PY79, cyclostrobin and water control. Each treatment group was inoculated with 15 wheat ears, and after inoculation, it was cultivated until the whole ear of the clear water treatment grou...

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Abstract

The invention provides pseudomonas chlororaphis for preventing and treating the crop fusarium disease and applications thereof. The preservation number of the pseudomonas chlororaphis is CGMCC No. 7729. The Pcho01 bacterium strain of the pseudomonas chlororaphis has a high effective antagonistic action, is capable of being applied to the preventing and treating wheat scab caused by fusarium, and the preventing and treating efficiency in the field is maintained above 60%. The Pcho01 bacterium strain also has a very good antagonistic action on botrytis cinerea, rice sheath blight fungus, bipolaris maydis, pythium wltmum, rhizopus, and pseudomonas solanacearum, and can be used to prevent and treat the diseases caused by those bacteria mentioned above.

Description

technical field [0001] The invention relates to the field of development and utilization of microbial germplasm resources, in particular to Pseudomonas chloropinus for preventing and treating crop Fusarium diseases and its application. Background technique [0002] Fusarium (Fusarium) belongs to the fungus of the subphylum Deuteromycotina, which is a kind of fungus distributed worldwide. It can not only survive winter and summer in the soil, but also infect a variety of plants (food crops, economic crops, medicinal plants and Ornamental plants), causing ear rot, root rot, stem rot, stem base rot, flower rot and other diseases of plants, with more than 100 species of host plants, infecting the vascular system of host plants and destroying the vascular bundles of plant transport tissues , and produce toxins in the process of growth, development and metabolism to harm crops, cause crop wilting and death, and affect yield and quality. Among them, the diseases caused by Fusarium...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01G7/06C12R1/38
Inventor 陈云尹燕妮马忠华
Owner ZHEJIANG UNIV
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