63 results about "Dahlia" patented technology

The invention discloses Bacillus subtilis, which is named Bacillus subtilis. The Bacillus subtilis was collected in China General Microbiological Culture Collection Center on January 15th, 2009, with a collection number of CGMCC No.2843. The Bacillus subtilis strain disclosed by the invention is screened from tobacco leaf habitats. Experiments prove that the fermentation liquor of the strain has a strong inhibition effect on fungal pathogens such as Alternaria alternate and pepper anthracnose and demonstrates high prevention effect on Alternaria alternate, tobacco black shank, pepper anthracnose, tomato wilt, phytophthora melonis Katsura, verticillium dahlia and the like and has high growth promoting effects on tobacco, pepper, cucumber, tomato and eggplant. Therefore, the Bacillus subtilis disclosed by the invention can be used for preparing biological bactericides for preventing and controlling fungi in tobacco and vegetables and can be used for preparing growth promoting agents for promoting the growth of tobacco or vegetables.

The invention discloses the application of potassium phosphorite as bactericidal agent. The potassium phosphorite possesses distinctive effect of killing gray botrytis and fusanin specialized for cucumber, fusanin specialized for watermelon, corn maize gibberellic disease, guamycin, grain sclerothricin, and sclerin and dahlia verticillin.

The invention discloses an application of Bacillus methylotrophicus ZBL-1 in control of cotton verticillium wilt. The Bacillus methylotrophicus ZBL-1 with CGMCC No.11636 and higher inhibition effect on verticillium dahlia is obtained through separation, screening, breeding and domestication of soil samples collected in the cotton fields with severe diseases in Shihezi city and is obviously different from common Bacillus methylotrophicus strains, and meanwhile, the control effect of the screened strain ZBL-1 on the cotton verticillium wilt in indoor pot culture experiments is 73.0%; the control effect of the screened strain ZBL-1 in field plot experiments is 43.1%, and whether in development of a new biopesticide or biological control agent, the Bacillus methylotrophicus ZBL-1 as a biological control material for the cotton verticillium wilt has very good application prospect and has realistic significance and functions.

The invention discloses paenibacillus polymyxa for preventing and treating vertieillium wilt in crops and application of the paenibacillus polymyxa. The strain is named as paenibacillus polymyxa SX-3, with preservation number as CGMCC 11638. The invention provides an inoculant containing the paenibacillus polymyxa. The strain of the invention has an inhibitory effect on both spore germination and mycelial growth of verticillium dahlia and the strain has an efficient antagonistic action. The strain of the invention is capable of preventing and treating crop verticillium wilt caused by the verticillium dahlia, the strain has a significant effect on preventing and treating cotton verticillium wilt and control efficacy of pot experiments reaches 86% or above; and bacterium liquid containing the strain of the invention, when irrigated to the roots of crops in a late disease period, can still achieve a significant therapeutic effect; therefore, the strain has a great potential in the application to bio-pesticides.

The present invention discloses a Chinese traditional medicine pill for treating small intestine hernia and testicle swell-fall. The making active ingredients: 1 to 5g of radix auckladiae, 1 to 2g of gmyrrh, 0.5 to 1g of frankincense, 1 to 3g of chuan aconite root, 1 to 3g of rhizome corydalis (vinegar), 0.5 to 1g of dahlia ginseng, 0.5 to 1g of scorpion, 0.5 to 3g of chuan Chinaberry fruit, 1.5 to 3g of common fennel and 1 to 6g haw kernel. The making technique: the medicines are made into fine flour, and then mixed with yellow wine to make one hundred pills. The medicine is a Chinese traditional medicine pill for treating small intestine hernia and testicle swell-fall which has low treatment cost, short treatment time, and no pain for patients and high cure rate.

The invention discloses a method for remarkably improving resistance of cotton to greensickness through verticillium dahlia interference VdRGS1 gene expression and belongs to the field of biological technique application. The invention provides a nucleotide sequence of a VdRGS1 gene and an ORF sequence and an amino acid sequence of cDNA. Function and phenotypic analysis shows that VdRGS1 is capable of regulating and controlling pathogenicity of verticillium dahlia, formation of microsclerotia, growth of spores and hyphae, and the like, and plays a significant role when verticillium dahlia invades and infect a host. By using a transgenic technique, a greensickness bacterium which can express green fluorescent protein (GFP) is created, by using a virus-induced gene silencing (VIGS) technique, a GFP gene of the transgenosis verticillium dahlia can be efficiently silenced in cotton, the result shows that host induced gene silencing (HIGS) is achieved on the basis of the VIGS technique, andinvading verticillium dahlia genes can be successfully silenced in cotton by using the technique. By using an HIGS technique based on VIGS, the verticillium dahlia VdRGS1 is silenced in cotton, and the resistance of cotton to verticillium dahlia is remarkably improved.

The invention relates to a method for regulating florescence of dwarf dahlia. The method comprises the steps of: starting supplementing light in the beginning of two months before the expected flowering; lighting for 14 hours every day; carrying out full light from 8:00 to 18:00 every day; discontinuously supplementing light by utilizing an LED (Light Emitting Diode) lamp from 18:00 to 3:00 the next day morning, irradiating for an hour every other two hours, increasing the light time to 4 hours in all; keeping in the darkness at other periods and maintaining the light intensity between 10000-15000Lx during the light periods; stopping supplementing light one month before the flowering; watering by utilizing 600 times liquid of Peters professional 12 once every 3 days; and stopping the watering of fertilizer water when flower buds present color, wherein the cultivation conditions are that the temperature is maintained at 17-20 DEG C during daytime, the temperature is maintained at 12-15 DEG C during nighttime, and the humidity is maintained between 70% and 75%.

The invention relates to phytopathology, and particularly relates to endophytic fungi CEF-193 of cotton and application thereof. The bacterial strain CEF-193 (Acremonium alternatum) with a significant induced immune function on cotton verticillium wilt is obtained from the endophytic fungi of the cotton by separation, screening, identifying and biological assay of a greenhouse, the preservation number is CGMCC No.8302, and CEF-193 can be cultivated on an artificial medium, and is easily obtained and cultivated. Thus, industrialized production and popularization and application are facilitated. An indoor bacteriostatic test shows that the control effect on greensickness is good, verticillium dahlia is inoculated 4 days after the CEF-193 is firstly inoculated, the prevention and control effect on the cotton verticillium wilt achieves 74.1%, and the endophytic fungi CEF-193 have significant control action on the greensickness. Compared with the bacillus subtilis mainly applied to production, the prevention and control effect is improved by about 40%, and the endophytic fungi CEF-193 are safe on people and livestock, insects and crops, and friendly to environment.

The invention discloses a bactericide composition. The bactericide composition contains pyrimethanil and berberine, for 1 part of pyrimethanil, the content of the berberine is 0.01-90 parts by weight. The invention further provides application of the bactericide composition in prevention and control of botrytis of cucumbers, tomatoes, strawberries, grapes, rieger begonia, cyclamen, cineraria, phalaenopsis, poinsettia pulcherrima, peony, China aster, camellia, chrysanthemums, begonia, dahlia, geranium and gladiolus. Through the adoption of the technical scheme, the bactericide composition has obviously enhanced prevention and control effect with respect to the pyrimethanil and berberine.

The present invention provides one new kind dahlia Streptoverticillium resisting receptor protein (S1Vc1) expressed in Solanum lycopersicoides Dun. and its coding sequence and application in improving plant disease resistance, especially in resisting verticillium wilt. The present invention relates to fusion gene constituent and new recombinant expression vector carrying the constituent, as well as transgenic plant generated via transforming plant cell with the expression vector and its subsequent generation, including plant seed and plant tissue. The present invention expresses the said gene in plant to obtain transgenic plant with strengthened resistance to plant diseases, especially verticillium wilt.

The invention discloses a medium for improving the antibacterial activity of biocontrol bacillus to plant pathogenic fungi, comprising the following components: potato, sucrose, maize paste, sodium L-glutamate, phosphate, MgSO4.7H2O, CaCl2, ferric citrate, MnSO4.H2O, phytic acid, L-proline, threonine, tetramethyl glutaric aciduria, gamma-aminobutyric acid, sodium nitroprusside, hydrocortisone, LaCl3, amino-oligosaccharin, chamomile leach liquor, and celery juice. A biocontrol bacillus seed solution is inoculated into the medium for temperature-variable fermentation culture, so as to promote the growth and reproduction of bacillus, and effectively stimulate the activity of bacillus, enhance the secretion of bacteriostatic substances and the activity of pathogen hydrolase, thereby significantly improving the antibacterial activity of bacillus to plant pathogenic fungi such as fusarium oxysporum, verticillium dahlia, botrytis cinerea, sclerotinia sclerotiorum, and providing a theoreticalbasis for the further development and application of biocontrol bacillus.

The present invention relates to the field of plant diseases, particularly to applications of verticillium dahlia pathogenicity related gene VdPR1 as the anti-verticillium dahlia target gene. According to the present invention, the colony morphology, the growth rate, the extracellular enzyme activity and the pathogenicity of the knockout mutant [delta]VdPR1 and the complementary mutant [delta]VdPR1-C are determined, and are subjected to compared analysis with various indexes of the wild type, such that it is determined that the VdPR1 gene is the verticillium dahlia pathogenicity related gene, is associated with microsclerotium production and melanin accumulation, and provides promotion effects for activities of cellulase and protease.

The invention discloses a regulation and control method for improving heat resistance of dahlia. The method includes: preparing a salicylic acid solution with the concentration of 0.1-1.5 mmol / L, spraying leaves with the salicylic acid solution in 1-2 days before high temperature stress during a growth period or a flowering period of the dahlia, continuously spraying the leaves for 2 days, and spraying the leaves once in the morning and evening every day respectively; and spraying the leaves once every 6-8 days during continuous high temperature stress. After the dahlia is sprayed with the salicylic acid solution, dahlia plants grow and develop in a relative normal state, and most of the dahlia plants grow well in a high temperature stress environment; leaves of the dahlia plants are dark green, and the chlorophyll relative content and the net photosynthetic rate are increased; the number of flowers of a single plant is increased, the flower diameter is increased, and the full-bloom period is prolonged; resistant physical signs such as autioxidant enzyme activities, malondialdehyde, proline and soluble proteins of the leaves of the dahlia change correspondingly, and then tolerance of the dahlia in the high temperature environment can be effectively improved, which has positive effects on introduction, open field cultivation and landscape application of the dahlia.

The invention discloses a germplasm preservation method of dahlia tissue culture propagation. The germplasm preservation method comprises the following technical links: 1) selection of an explant and low temperature pretreatment; 2) inoculation; 3) adventitious bud induction and transfer; 4) slow growth and germplasm preservation; 5) rejuvenation; 6) rooting and transplantation. According to the germplasm preservation method of dahlia tissue culture propagation provided by the invention, the germplasm preservation cycle of dahlia is up to 4 years, meanwhile the problems that the occupied area of the germplasm preservation is large, the cost is high and the like are solved, and the operation is simple, the technology is stable and reliable, the repeatability is high, the germplasm preservation method is very suitable for the germplasm preservation of large-scale dahlia, and is an excellent germplasm resource preservation method of the dahlia.

The invention relates to the field of the pathogenesis mechanism of Verticillium dahliae in cotton and specifically relates to a pathogenicity related protein of Verticillium dahliae in cotton and itscoding gene, application and mutant. The coding gene has a nucleotide sequence shown in the formula of SEQ ID No. 2. The low pathogenicity mutant T1027 is screened and has a growth rate the basicallysame to that of the wild body and a sporulation capability and secretion of crude toxins significantly lower than those of the wild body. An identification result shows that the T-DNA-inserted gene can code dextranase. The mutant T1027 of Verticillium dahliae in cotton is an ideal strain for researching the Verticillium dahlia molecular pathogenesis. The expression and modification of the gene and protein can be used as target sites for the design and screening of antifungal agents and have the good potential for application.

The invention relates to a cottage breeding method of dahlia pinnata. The cottage breeding method of dahlia pinnate comprises the steps of (1) selecting cuttings; (2) pruning cutting wounds of dahlia pinnata; (3) disinfecting; (4) temperature processing; (5) matrix preparing; (6) cutting; (7) controlling growth conditions: putting the cuttings obtained in step (6) in greenhouse with temperature of 20-33 DEG C, air humidity of 85-92%, and illumination of 550-700 lx. The cottage breeding method of dahlia pinnata refers to a seedling method that a portion of vegetative organs of dahlia pinnata is taken as the propagation material, and the propagation material is inserted in to a matrix with a certain proportion of garden soil, coir, vermiculite and perlite. The method promotes rooting, germinating, and growing into complete plants. The cuttings can preserve hereditary of the mother plants, can grow, develop, and show the same traits as the mother plants under suitable conditions, and significantly improve the survival rate of breeding of dahlia pinnata.

The invention discloses a combined firework. The combined firework comprises a firework powder barrel and powder filling the firework powder barrel. The combined firework setting-off effect is attractive and diversified, and specifically comprises red star white glitter mine shooting up brocade crown and red plum blossom, blue star titanium flower crackling mine shooting up orange purple dahlia silver plum blossom, titanium chrysant mine shooting up aquamarine blue dahlia gold plum blossom, red mine shooting up red plum blossom titanium chrysant ejecting brocade crown, titanium flower crackling mine shooting up gold plum blossom blue ejecting red palm and white glitter mine shooting up red blue dahlia and silver plum blossom ejecting purple palm. The setting-off effect is extremely attractive, and the development of the color diversity of the fireworks is promoted.

The invention relates to a method for obtaining endophytic bacteria for degrading verticillium dahlia toxin protein and an application in preventing and treating plant verticillium wilt, and belongs to the biotechnological field. The method for obtaining endophytic bacteria for degrading verticillium dahlia toxin protein comprises the steps of culturing endophytic bacteria separated from the plant and verticillium dahlia toxin; using polyacrylamide gel electrophoresis to detect protein band after culture, comparing with protein band of the original toxin; and taking mixed culture system without 26KD protein band as a target strain. The method has clear target and mechanism. The method overcomes the shortcomings that the bio-control microorganism in biological control soil borne disease is easily influenced by soil environment and has unstable effect. The method uses pathogenic character of the verticillium dahlia and conduction characteristic of the endophytic bacteria in the plant to directly screen endophytic bacteria for grading verticillium wilt toxin result in plant disease so as to achieve the aim of effectively preventing and treating plant verticillium wilt.

The invention discloses a verticillum dahlia resistant composition which comprises, by weight parts, 10-15 parts of 12% potassium sulfate, 8-12 parts of 55% organocopper complex-aliette, 10-15 parts of 6% calcium nitrate, 4-8 parts of citric acid, 4-8 parts of pulsatilla saponin, 6-10 parts of glycyrrhizic acid, 4-8 parts of ginger juice and 6-10 parts of 2% glutaraldehyde. The verticillum dahlia resistant composition has simplicity in matching and is convenient to use and good in eggplant verticillium wilt treatment effect. According to tests, 400-600-fold solution of the composition has obvious inactivation effects on fungi imperfecti subdivision dahlia verticillium.

The invention discloses a dahlia cultivation method. The dahlia cultivation method comprises the following steps: 1) explant selection and disinfection: taking young shoot and removing leaves, cutting the material into segments with buds, disinfecting the stem segments for standby application; 2) primary induction culture: inoculating the disinfected stem segments to a primary induction medium for culture; 3) subculture multiplication culture: cutting new buds after the buds are grown, inoculating the new buds to a subculture multiplication medium for culture; 4) rooting induction: after single bud spreading, segmenting the single bud to a rooting medium for root induction; 5) hardening seedling of rooted seedlings: through rooting induction, after rooting induced by sprout, performing hardening seedling and transplanting; and 6) tissue culture plants transplanting and management. The method has the advantages of simple operation, short time, low cost, high survival rate, and high rooting rate; the bottle transplanting in a short time can be realized, the tissue culture method can keep excellent property of an original type of dahlia, and establishes a base for seed breeding and industrial production.

The invention discloses a verticillium wilt resistant associated protein GbVIP1, a coding gene and application thereof. The amino acid sequence of the protein is shown as SEQ ID NO:1. The protein GbVIP1 provided by the invention comes from the verticillium wilt resistant gossypium barbadense variety Hai7124, belongs to the subfamily I of bZIP transcription factor family, and has a conservative bZIP structural domain. The study of the invention finds that the GbVIP1 gene has up-regulated expression under a verticillium dahlia infection condition, and silencing of the GbVIP1 gene lowers the resistance of gossypium barbadense to verticillium dahlia, therefore the GbVIP1 protein has important significance for verticillium wilt resistant breeding of cotton.

The invention discloses a preparation method of a strawberry verticillium dahlia spore suspension. The method comprises the following steps of collecting strawberry verticillium dahlia infected plants; performing separation and purification; screening out pathogenic bacteria of the strawberry verticillium wilt; inoculating the separated verticillium dahlia into a PDA culture medium to perform plate culture and activation; taking down an agar block containing activated pathogenic bacteria; adding the agar block into a mixture formed by a liquid VL culture medium and 2.0-percent saccharose for shake culture; performing gauze filtering and filter paper vacuum pumping filtering for removing rest hyphae to obtain filter liquid for use; centrifuging filter liquid; abandoning supernate; and performing resuspension and sedimentation by 2ml of sterile water to obtain the strawberry verticillium dahlia spore suspension. The liquid culture medium inoculation and culture mode is used, so that the full growth all over the whole liquid culture medium can be fast achieved; and the labor consumption and the material consumption are reduced. The preparation method has the advantages that the inoculation amount is small; the spore generation speed is high; the growth is fast; the differences of spore quantities prepared from the same pathogenic bacteria in the same batch and different batches are small; and the method stability is high.

The invention belongs to the technical field of genetic engineering and particularly relates to application of sea island cotton GbNAC1 gene in verticillium wilt resistance. The GenBank ID of a GbNAC1 gene is KP317496, and the gene is relevant to the verticillium wilt resistance of plants. The inventor finds that when the plants are infected by verticillium dahlia, the expression quantity of the gene is reduced, the verticillium wilt resistance of the plants is reduced after the gene is further silenced, and the verticillium wilt resistance of the plants is improved after the gene overexpression. Based on the characteristics, GbNAC1 overexpression plants can be cultivated or screened, better references can be provided for new varieties of plants having better verticillium wilt resistance, and accordingly the sea island cotton GbNAC1 has a better application value on the aspect of cultivation of new varieties of plants.

The invention discloses a using method of a drug for resisting verticillium wilt of eggplants. The using method comprises the following steps: A, preparing a basic solution comprising the following components: 50% wettable carbendazol powder, 77% wettable kocide powder, 90% wettable hymexazol powder, indirubin, matrine, chlorogenic acid, radix scutellariae and coriander; B, cultivating eggplant varieties with high disease resistance, such as fast round eggplant, Tianjin Damin eggplant, Ermin eggplant, purple big round eggplant and the like, soaking eggplant seeds in 400-fold volume of basic solution for 1.5-2 hours, taking the eggplant seeds out, rinsing the eggplant seeds with clear water, and planting; C, in case of the verticillium wilt, adding water to dilute the basic solution to 600 folds for spraying; D, adding water to dilute the basic solution to 500 folds for irrigating roots at the same time of spraying in the step C. The using method is simple; used components are simple in matching; the using method is convenient; effect of treating the verticillium wilt of the eggplants is good; tests show that 400-600-fold solution of the mixture has an obvious inactivating effect on verticillin of deuteromycotina dahlia, and a good bactericidal effect.

The invention relates to an LRK2 gene giving greensickness resistance to plants and an application thereof, which belongs to the technical field of biology. The LRK2 gene is a surface receptor protein gene acquired from an upland cotton variety AO3503, the length of a complete coding frame of the LRK2 is 2016 basic groups, a coding protein contains 671 amino acids, the molecular weight is 74KD, and an isoelectric point is 7.85. The disease resistance of transgenic arabidopsis to defoliating type verticillium dahlia V991 and non-defoliating type verticillium dahlia BP2 is obviously enhanced, the disease rate of the transgenic arabidopsis is lower than 20 percent, and the disease rate of a reference wild type is higher than 50 percent.

The invention relates to dahlia purple oral liquid for an initial-period animal warm heat disease. The dahlia purple oral liquid comprises, by weight, 0.8-1.2 parts of herba andrographitis, 0.8-1.2 parts of folium isatidis and 1.6-2.4 parts of radix isatidis. The invention further relates to a preparing method of the dahlia purple oral liquid for the initial-period animal warm heat disease. Extraction is carried out on the herba andrographitis through 75% ethyl alcohol two times at 40 DEG C; the folium isatidis and the radix isatidis are soaked with purified water firstly for water decoction and alcohol sedimentation extraction; extraction of the dahlia purple oral liquid is efficient and complete, the component is simplified, and the production cost is reduced; and effective ingredients such as the herba andrographitis, the folium isatidis and the radix isatidis are simultaneously used to the maximum degree.

The invention provides nucleic acid which is provided with a sequence as shown in SEQ ID NO:1. The invention further provides a primer pair for amplifying the nucleic acid and a method for identifying a pathotype of a fungi strain. The fungi strain is verticillium dahlia. The method comprises the following steps: (1) preparing a sample to be tested, containing whole genome DNA (deoxyribonucleic acid) of the verticillium dahlia; (2) taking the sample to be tested, containing whole genome DNA obtained from the step (1) as a template and carrying out PCR (polymerase chain reaction) amplification by the primer pair; detecting if nucleic acid as shown in SEQ ID NO:4 is existent in an amplified product; (3) judging the pathotype of the verticillium dahlia according to a detection result in the step (2), and when nucleic acid as shown in SEQ ID NO:4 is inexistent in the amplified product, indicating that the pathotype of the verticillium dahlia belongs to a high pathotype; when nucleic acid as shown in SEQ ID NO:4 is existent in the amplified product, indicating that the pathotype of the verticillium dahlia does not belong to the high pathotype.

The invention relates to application of ambrosia artemisiifolia essential oil as a verticillium dahliae bactericide. Growth of verticillium dahliae is inhibited by a method of enabling the verticillium dahlia to be in contact with the ambrosia artemisiifolia essential oil and fumigating the verticillium dahlia with the ambrosia artemisiifolia essential oil. Results show that the ambrosia artemisiifolia essential oil has a significant effect on mycelial growth of the verticillium dahliae at a low concentration of 0.5 mu l / ml, and the growth inhibition effect is improved gradually with the increase of the concentration; when the concentration is continuously increased to be 5 mu l / ml, the mycelia of inoculated stems all lose the activity of continuous expansion, and the growth of the myceliais completely inhibited; in a fumigation experiment, the ambrosia artemisiifolia essential oil with the concentration of 0.2 mu l / ml fumigates the stems of the verticillium dahlia, and the mycelia ofthe stems are significantly inhibited; and when the concentration reaches 1.2 mu l / ml, the mycelia are killed completely to lose activity. The growth of the verticillium dahliae is inhibited by the ambrosia artemisiifolia essential oil provided by the invention, the ambrosia artemisiifolia essential oil is derived from plants, can be decomposed in nature, is environmentally friendly and does notpollute the environment, and meanwhile, waste harmful plants are utilized.

The invention provides a nested PCR detection kit. The kit comprises a primer P1 and a primer P2 for first-round PCR amplification, and a primer PN1 and a primer PN2 for second-round PCR amplification, wherein the primers P1, P2, PN1 and PN2 have the same or similar amplification functions shown as SEQ ID NO. 1-4 respectively in PCR amplification reactions. The invention further provides the application of the kit to detection of the verticillium dahlia. The invention further provides a method for detecting the verticillium dahlia by using the kit. The kit can quickly detect the verticillium dahlia with high sensitivity and high accuracy.