Dahlia cultivation method

A cultivation method and dahlia technology, applied in the field of plant cultivation, can solve the problems of difficult survival of breeding, easy browning of explants, and low survival rate, and achieve the effects of low cultivation cost, good promotion and application prospects, and high plant rooting rate.

Inactive Publication Date: 2017-08-29
HEFEI XUELIXIN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These cultivation methods take a long time, and the breeding is not easy to survive
Some existing dahlia tissue culture methods are carried out under closed aseptic conditions, and the required cost is relatively large, and there is also the problem that the explants are easy to brown in tissue culture and the survival rate is not high, so they cannot be used for dahlia. Multiply

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The following cultivation process is mainly carried out in the cultivation room, and the cultivation conditions of primary induction culture, subculture proliferation culture and rooting induction are as follows: ambient temperature 22°C, light time 10h / d, and light intensity 1600lx.

[0032] A kind of cultivation method of dahlia is carried out according to the following steps:

[0033] (1) Selection and disinfection of explants: Take the young shoots and remove the leaves, cut into 4cm, and each section has at least one axillary bud stem section, the stem section is first sterilized with 600 times of sterilized liquid for 25 minutes, aseptic Wash with water twice, then use 0.1% mercury liter, 2 drops of Tween 80, treat for 3 minutes, wash with sterile water twice, and use after disinfection;

[0034] (2) First-generation induction culture: Inoculate the sterilized stem segment in step (1) on the first-generation induction culture. After 8 days of cultivation, new shoo...

Embodiment 2

[0049] The following cultivation process is mainly carried out in the cultivation room, and the cultivation conditions of primary induction culture, subculture proliferation culture and rooting induction are as follows: ambient temperature 23°C, light time 12h / d, and light intensity 1800lx.

[0050] A kind of cultivation method of dahlia is carried out according to the following steps:

[0051] (1) Selection and disinfection of explants: remove the leaves from the young shoots, cut them into 5cm sections, and each section has at least one axillary bud. Wash with water for 3 times, then use 0.1% mercury liter and 3 drops of Tween 80 for 4 minutes, wash with sterile water for 3 times, and use after disinfection;

[0052] (2) First-generation induction culture: inoculate the sterilized stem section in step (1) on the first-generation induction culture, and after 10 days of cultivation, new shoots will germinate and grow from the axillary buds. The induction medium is DCR modified...

Embodiment 3

[0067] The following cultivation process is mainly carried out in the cultivation room, and the cultivation conditions of primary induction culture, subculture proliferation culture and rooting induction are as follows: ambient temperature 24°C, light time 14h / d, and light intensity 2000lx.

[0068] A kind of cultivation method of dahlia is carried out according to the following steps:

[0069] (1) Selection and disinfection of explants: remove the leaves from the young shoots, cut them into 6cm sections, and each section has at least one axillary bud. Wash 4 times with water, then use 0.1% mercury liter, 5 drops of Tween 80, treat for 5 minutes, wash 4 times with sterile water, and use after disinfection;

[0070] (2) First-generation induction culture: inoculate the sterilized stem section in step (1) on the first-generation induction culture, and after 12 days of cultivation, new shoots will germinate and grow from the axillary buds. The induction medium is DCR modified + 6...

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PUM

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Abstract

The invention discloses a dahlia cultivation method. The dahlia cultivation method comprises the following steps: 1) explant selection and disinfection: taking young shoot and removing leaves, cutting the material into segments with buds, disinfecting the stem segments for standby application; 2) primary induction culture: inoculating the disinfected stem segments to a primary induction medium for culture; 3) subculture multiplication culture: cutting new buds after the buds are grown, inoculating the new buds to a subculture multiplication medium for culture; 4) rooting induction: after single bud spreading, segmenting the single bud to a rooting medium for root induction; 5) hardening seedling of rooted seedlings: through rooting induction, after rooting induced by sprout, performing hardening seedling and transplanting; and 6) tissue culture plants transplanting and management. The method has the advantages of simple operation, short time, low cost, high survival rate, and high rooting rate; the bottle transplanting in a short time can be realized, the tissue culture method can keep excellent property of an original type of dahlia, and establishes a base for seed breeding and industrial production.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a dahlia cultivation method. Background technique [0002] Dahlia, also known as dahlia, geranium peony, oriental chrysanthemum, dahlia, sweet potato flower, is a plant of the family Asteraceae and dahlia, a perennial herb with huge stick-shaped roots. Stem erect, much branched, 1.5-2 meters high, strong. Originated in Mexico, Mexicans regard it as a symbol of generosity and wealth, so they respect it as the national flower. At present, it is planted in most countries in the world, and new varieties have been bred from time to time. According to statistics, there are more than 30,000 varieties of dahlias, which is one of the species with the most varieties of flowers in the world. Dahlia is one of the famous flowers in the world due to its variety of colors and shapes, rich and varied names. It can also promote blood circulation and dissipate blood stasis, so it has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张力
Owner HEFEI XUELIXIN AGRI TECH CO LTD
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