LRK2 gene giving greensickness resistance to plants and application thereof

A Verticillium wilt and gene technology, applied in the field of plant genetic engineering, can solve the problems that the plant growth and development resistance effect has not reached the expected goal, and achieve the effect of enriching resistance gene resources and improving disease resistance traits

Inactive Publication Date: 2016-05-18
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES +1
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Problems solved by technology

In addition, Xanthomonas oryzae harpin protein, chitinase gene, lipid transferase gene, Gastrodia elata antifungal protein, antimicrobial peptide, defensins (Miaoetal., 2010; Munis, 2010; NiMetal., 2013) also showed certain resistance to Verticillium wilt, but because the overexpression of defense genes brought some adverse effects on plant growth and development, and the resistance effect did not reach the expected goal, etc., thus limiting the use of these genes in production. application

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  • LRK2 gene giving greensickness resistance to plants and application thereof
  • LRK2 gene giving greensickness resistance to plants and application thereof
  • LRK2 gene giving greensickness resistance to plants and application thereof

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[0033]The methods used in the following embodiments are conventional methods unless otherwise specified, and the primer sequences used are all synthesized by Shanghai Yingjun Biotechnology Co., Ltd. The gene in this experiment was derived from the upland cotton variety Ao 3503 (Gossypium hirsutum) (Liu Xiaoshuang, Liu Tingli, Yuan Hongbo, Zhang Baolong, Wang Rongfu. Analysis of drought tolerance function of cotton natriuretic peptide gene GhPNP1. Chinese Agricultural Sciences, 2015,48(12):2306 -2316).

[0034] (1) Cloning and sequence analysis of cotton LRK2

[0035] Primers were designed according to the Raymond cotton LYK2-Like gene (Gossypium raimondii, protein LYK2-like, accession number XM_012602491)

[0036] P1: 5'-TGCTCTAGAATGGCATCTGTAATGAGTAA-3',

[0037] P2:5'-CGCGGATCCTCACAGATTGGATGTTGTG-3'

[0038] The cDNA template of the upland cotton variety Ao 3503 was amplified, and 1 μl of the cDNA template was added to the 25 μl reaction system, each primer was 5 nmol, and...

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Abstract

The invention relates to an LRK2 gene giving greensickness resistance to plants and an application thereof, which belongs to the technical field of biology. The LRK2 gene is a surface receptor protein gene acquired from an upland cotton variety AO3503, the length of a complete coding frame of the LRK2 is 2016 basic groups, a coding protein contains 671 amino acids, the molecular weight is 74KD, and an isoelectric point is 7.85. The disease resistance of transgenic arabidopsis to defoliating type verticillium dahlia V991 and non-defoliating type verticillium dahlia BP2 is obviously enhanced, the disease rate of the transgenic arabidopsis is lower than 20 percent, and the disease rate of a reference wild type is higher than 50 percent.

Description

1. Technical field [0001] The invention provides a LRK2 gene for endowing plant with Verticillium wilt resistance and its application, belonging to the field of plant genetic engineering. It is used to improve plant disease resistance and other beneficial production traits through plant genetic engineering technology. 2. Background technology [0002] At present, verticillium wilt is very serious in production, and it is the main fungal disease of cotton, tomato, pepper, eggplant and other plants, resulting in reduced production or even no harvest. There are no effective control measures. The pathogen of Verticillium wilt is mainly Verticillium dahliae, which exists in the soil for a long time in the form of microsclerotia. Verticillium dahliae will show pathogenicity differences on host plants, and under the influence of host interaction, co-evolution and ecological environment differences, it often produces physiological differentiation and new pathogenic types. Zhang Ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00
CPCC12N15/8282C07K14/415
Inventor 刘廷利顾周航周雪平张保龙杨郁文陈天子凌溪铁王金彦
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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