Molecular marker closely linked with major QTL (Quantitative Trait Loci) for resisting gray leaf spot of corn and application of molecular marker

A molecular marker, gray spot disease technology, applied in the fields of genetic engineering and molecular biology, can solve problems such as yield loss, and achieve the effects of high contribution rate, improved disease resistance traits, and convenient and fast detection methods

Pending Publication Date: 2022-05-13
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Yield loss between 10%-70%

Method used

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  • Molecular marker closely linked with major QTL (Quantitative Trait Loci) for resisting gray leaf spot of corn and application of molecular marker
  • Molecular marker closely linked with major QTL (Quantitative Trait Loci) for resisting gray leaf spot of corn and application of molecular marker

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1: Acquisition of linked marker molecular markers for the main QTL of resistance to gray spot disease in maize

[0027] 1. Construction of fine positioning groups

[0028] In 2015, the main effect QTLqGLS1.02 was obtained from the RILs derived from the resistant parent Qi 319 and the susceptible parent Ye 478, such as figure 1 Shown, and select the resistant individual and the susceptible parent Ye 478 for backcrossing.

[0029] All maize plants were inoculated to ensure the concentration of bacteria and the accuracy of single plant phenotype, and the inoculation effect was identified 20 days after pollination.

[0030] 2. Take healthy leaves, about 16cm per plant 2 , Genomic DNA was extracted by the modified CTAB method.

[0031] 3. Primer Development and Synthesis

[0032] The primers used in the present invention were obtained from maizeGDB according to the reference genome of maize B73. And used to amplify the genomic DNA of the resistant parent Qi 319 ...

Embodiment 2

[0036] Example 2: Application of Molecular Marker COP-M1 in Improving Zhengdan 958 gray spot disease resistance

[0037] 1. Use the primers shown in SEQ ID NO.1 and SEQ ID NO.2 to amplify a band with a size of 185bp in the corn susceptible inbred lines Zheng 58 and Chang 7-2, and the nucleotide sequence is as shown in SEQ ID NO.2 ID NO.4 is shown.

[0038] 2. Using the molecular marker COP-M1, the main QTL for disease resistance from Qi 319 (male parent) was crossed with Zheng 58 (female parent) and Chang 7-2 (female parent) and backcrossed continuously. The leaf DNA of the offspring was extracted and amplified with the molecular marker COP-M1. Among the offspring, the offspring that amplified the 191bp band consistent with the Qi 319 band pattern were selected.

[0039] 3. The progeny selected according to the method in step 1 of Example 2 continued to serve as the female parent, and Qi 319 was used as the male parent for hybridization. Repeat step 1. The improved inbred l...

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Abstract

The invention discloses a molecular marker closely linked with a corn gray leaf spot resistant major QTL (quantitative trait locus), the corn gray leaf spot resistant major QTL is located at a position Bin1.02 of a first chromosome of corn and is named as QTLqGLS1.02, the molecular marker closely linked with the corn gray leaf spot resistant major QTL is COP-M1, and the physical position of the COP-M1 is Chromosome1: 23820258-23820275. According to the research, a molecular marker COP-M1 is developed on the gray leaf spot resisting major QTL of the corn chromosome 1 for the first time. The main effect QTL point for resisting the corn gray speck disease is clear in position and high in contribution rate, the disease resistance character of a corn inbred line material can be remarkably improved, the detection method is convenient and rapid, and the method can be effectively applied to identification of gray speck disease resistant materials and improvement of commercialized susceptible varieties.

Description

technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, in particular to a molecular marker closely linked with the main effect QTL for resistance to gray spot disease of maize and its application. Background technique [0002] Corn is an important grain and feed crop. China is not only a big corn producing country, but also a big consuming country. Maize gray spot caused by Cercospora infecting maize leaves has become one of the important diseases in the maize producing areas of Northeast, North and Southwest my country. Among them, the pathogen causing gray spot disease in Northeast China is Cercospora zeae, and the pathogen causing gray spot disease in Southwest China is Cercospora zeae (Liu Wenkui et al., 2013). The disease can occur throughout the growth period, but it is most serious in the tasseling and filling stages, and mainly harms the leaves of corn. Yield loss is between 10%-70%. Excavating resistant source mat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 翁建峰程子祥吕香玲李新海宋新元路明宋茂兴周志强李明顺郝转芳张德贵雍红军韩洁楠
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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