The invention relates to a trifloxystrobin and bupirimate-containing sterilization composition. The trifloxystrobin and bupirimate-containing sterilization composition is compounded by the active ingredients: trifloxystrobin and bupirimate in a binary compounding manner, wherein the mass ratio of the trifloxystrobin to bupirimate is (1-40):(40-1), the mass parts ratio of trifloxystrobin to bupirimate in preparation is 1%-80%, and the rest ingredients include auxiliary compositions allowable and acceptable in the pesticides. The forms of the sterilization composition comprise emulsifiable solution, suspending agent, wettable powder, water dispersible granules, emulsion in water, microemulsion, granules and microcapsules. The sterilization composition has good activity on almost all eumycetes (ascomycetes, basidiomycetes, oomycetes and fungi imperfecti) diseases such as powdery mildew, rust disease, sheath blight disease, net blotch, downy mildew, rice blast and the like, as well as good activity on damping off and apple scab.