The invention discloses a nematicidal composition containing fluopyram and a nicotine insecticide and an application thereof. The nematicidal composition is composed of a first active component, namely the fluopyram, a second active component, namely one of the nicotine insecticides thiamethoxam, imidacloprid, dinotefuran and sulfoxaflor, and an auxiliary agent, wherein the weight ratio of the first active component to the second active component is (1:20)-(20:1); the content sum of the first active component and the second active component is 1%-60% of the total weight of the composition. The composition can be made into granules, water dispersible granules, missible oil, an emulsion in water and a suspending agent which are agriculturally allowed. The components are reasonably compounded, the effect on vegetable nematode is good, and the nematicidal effect is not simply superposed by the activities of all the components. Compared with a conventional single preparation, the nematicidal composition has a remarkable nematicidal effect and a remarkable synergistic effect, has the dosage reduced, and has good safety to crops.