The invention discloses a metconazole and tebuconazole containing bactericidal composition and application thereof. Main ingredients include metconazole and tebuconazole according to the ratio of 1: 40 to 20:1, and a proper auxiliary and a carrier are used in cooperation for prevention and treatment of diseases of wheat. Compared with traditional chemical agents, the composition has the advantages that the two main ingredients are compatible, the synergistic effect is remarkable, bactericidal activity is improved, the dosage of single fungicide is reduced, and pesticide cost is reduced; the composition is efficient, low in toxicity, low in residue quantity, good in fast-acting property, long in lasting period and environmentally friendly; the chemical resistance risk level of pathogenic bacteria to chemical agents is lowered, maintenance of sensitivity of the pathogenic bacteria is benefited, and meanwhile chemical resistance of bacteria to a single dosage in the formula can be delayed; the bactericidal effect is remarkable, wheat scabs are effectively prevented and treated, wheat powdery mildews, wheat rust, wheat sharp eyespots and wheat leaf blotches are treated at the same time, chemical resistance of the pathogenic bacteria to carbendazim is governed, harm caused by diseases to crop production is reduced, the yield of agricultural products is raised, and the quality of agricultural products is improved.