227 results about "Dimethoate" patented technology

The invention relates to a method for culturing submerged plant seeds. The method comprises the following steps: A, soaking the submerged plant seeds produced by apomixia in 0.01-1wt% of dimethoate or sodium hypochlorite solution for 1-6 hours, washing the soaked submerged plant seeds with clear water, naturally drying the soaked submerged plant seeds in the air; B, standing the submerged plant seeds at 20-25 DEG C for 1-3 days, storing at 1-5 DEG C for 1-2 days, and preserving at 30-40 DEG C for 12-36 hours; C, soaking the submerged plant seeds in solution containing plant hormone, trace elements and inorganic salt for 12-24 hours; and D, burying the submerged plant seeds in a matrix by a depth of 3-5cm, and then putting the matrix in which the submerged plant seeds are buried at the bottom of river or lake water. The matrix comprises humus, sediment at the bottom of river and lake, bank soil, clay and sand. The submerged plant seeds bred by the method have high germination rate and environmental adaptability.

The invention discloses a method for degrading an organophosphorus pesticide based on a sweep-frequency double-frequency ultrasonic technology, and relates to the fields of agricultural environment and water protection. The method comprises the following steps of: putting an aqueous solution of the organophosphorus pesticide dimethoate into a reactor of equipment; and by setting different frequencies and different sweep frequency periods of a single frequency or a double frequency, setting the total volume of the aqueous solution of the organophosphorus pesticide dimethoate in the reactor, and performing sweep-frequency ultrasonic treatment at 25DEG C fro 40 to 180 minutes, wherein the concentration of the organophosphorus pesticide is 1.8-5.4mg / L. Toxic and harmful pollutants are degraded by the ultrasonic technology to the greatest extent, and the method has the advantages of high efficiency, no secondary pollution and energy conservation.

The invention discloses a bacillus cereus strain, which is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO:M209001. The invention also discloses a pyrethroid pesticide degrading enzyme prepared by using the bacillus cereus strain as an initial strain and a method for preparing the same. The strain is a bacillus cereus which is separated from the body of a tea plant and has high pyrethroid pesticide degradation efficiency. The intracellular crude enzyme of the strain has high efficiency in the degradation of pyrethroid pesticides such as cypermethrin, cyfluthrin and fenpropathrin, but low efficiency in the degradation of organophosphorus pesticides such as cygon, panaplate, methamidophos, chlorpyrifos and the like.

The invention discloses a preparation method of a dimethoate MIP, which comprises the following steps of: (1) mixing dimethoate as a template molecule, a functional monomer for polymerization, a cross-linking agent and pore former; (2) carrying out in-situ polymerization reaction of the mixture; (3) grinding the polymer, extracting and cleaning till the template molecule cannot be detected in the washed substance; and (4) drying the obtained MIP after the template molecule is removed in vacuum to obtain the dimethoate MIP. The invention further discloses a use method of the dimethoate MIP. The sample pre-treatment is carried out with a molecule imprinting technology. The content of dimethoate is measured by taking calcein-Pd2+ as a fluorescent probe through a fluorophotometry. The dimethoate MIP prepared by the method disclosed by the invention has higher adsorption rate on dimethoate, so that the interference of other substances on dimethoate is avoided.

The invention discloses a hot fogging concentrate containing cypermethrin, which is prepared by using cypermethrin or the mixture of cypermethrin and an active component II as the effective constituent, and adding accessory ingredient and solvent, wherein the active component II is any one of chlorpyrifos, dimethoate and nitenpyram. By using the cypermethrin as the effective constituent, the hot fogging concentrate comprises the following components by weight percent: 1.6-30% of cypermethrin, 1-20% of accessory ingredient and the balance of solvent. By using any one of the cypermethrin and the active component II as the effective constituent, the hot fogging concentrate comprises the following components by weight percent: 0.1-20% of cypermethrin, 0.1-40% of active component II, 1-20% of accessory ingredient and the balance of solvent. The hot fogging concentrate disclosed by the invention is applicable to a hot fogging machine for preventing insect pests on sugarcane, corn, tobacco, fruit trees and forests. The hot fogging concentrate has the advantages of high working efficiency, strong knock-down activity, synergetic effect, good preventing effect, simpleness in operation, water and labor saving, safety, environmental friendliness, and the like.

The invention discloses a preparation method of a micro-enzyme reactor based on magnetic functionalized graphene oxide, and application of the micro-enzyme reactor in pesticide detection, belonging to the technical field of micro-fluidic control chips. The micro-enzyme reactor which can be applied to pesticide detection is prepared by firstly, loading Fe3O4 magnetic nano particles on the surface of graphene oxide by using a one-step in-situ synthesis method, so as to prepare a graphene oxide / Fe3O4 nano composite material with both good magnetism and biocompatibility, secondly, fixing acetyl choline esterase on the surface of the graphene oxide / Fe3O4 through action of pi-pi, hydrogen bonds and water repelling and the like, and fixing a graphene oxide / Fe3O4 / acetyl choline esterase compound inside a PDMS (Polydimethylsiloxane) micro chip channel through the action of an external magnetic field. Based on the inhibition principle of pesticide to acetyl choline esterase, the rapid quantitative detection on dimethoate is realized. The micro-enzyme reactor which is prepared based on a graphene oxide / Fe3O4 / acetyl choline esterase composite material is rapid in action, simple to operate and good in reproducibility, and an effective means is provided for rapid and sensitive detection on pesticide.

The invention relates to a gas chromatographic method for detecting the residue amount of nine kinds of organophosphorus pesticide in rice. Qualitative and quantitative detection on the pesticide residues of dichlorphos, acephate, omethoate, dimethoate, phorate, malathion, fenitrothion, isocarbophos and triazophos is achieved. The gas chromatographic method is characterized in that acetonitrile is used as extractant, extracting solutions are concentrated through a gas blowing concentrator, and after volume metering is carried out through acetone, trace analysis is directly carried out on a gas chromatograph. The gas chromatographic method has the advantages that the separating effect of a chromatographic column on a target object is good, the detection result is high in accuracy, reproducibility is good, and method sensitivity meets the technical requirement of multi-pesticide-residue detection; compared with the method of GB / T5009.145-2003 national standards (the detection of multiple kinds of residues of organophosphorus and carbamates pesticide in plant food), the gas chromatographic method is simpler, simplifies the pre-processing processes of samples, shortens detection time, and saves cost.

The invention discloses a method for synthesizing dimethoate, which takes microcosmic salt and methyl chloroacetate as material to synthesize intermediate phosphate ester. The invention chooses phosphate ester and aminomethane as material to synthesize dimethoate. The reaction reacts in homogeneous system of organic solvent for 80-160 minutes in temperature of 0-60 DEG C. The invention synthesizes dimethoate in temperature of -10-5 DEG C for 60-150 minutes. Said microcosmic salt comprises ammonium salt, sodium salt, potassium salt and lithium salt. Said solvent comprises carbinol, alcohol, acetone, alcohol isopropylicum and tetrahydrofuran. The invention possesses carry out homogeneous phase reaction system to produce dimethoate with gross yield of up to 30% and pureness of 90% under the condition of existing device and material.

The invention discloses a cultivation method for comprehensively preventing peanut disease and pest injury and continuous cropping obstacles, and belongs to the technical field of peanut high-yield high-efficiency cultivation. The cultivation method mainly includes the cultivation steps: selecting high-resistance varieties; applying organic manure, carbon-based special slow-released fertilizers for peanuts and agricultural microbiological bactericides; dressing seeds by 50% of ethylicin, 40% of rogor emulsifying oil, 50% of phoxim and 0.4% of ammonium molybdate; spraying 50% of thiophanate methyl turbid liquid, 75% of chlorothalonil, 3.6% of acetic acid and high iodine, 10% of quizalofop-p-ethyl cream, 10.8% of high-efficiency haloxyfop-methyl cream and 70% of Seoul cream after seeding and earthing. By the method, peanut disease and pest injury and the continuous cropping obstacles can be prevented once in the cultivation process of the peanuts, the cultivation method is simple, manpower and material resources are saved, and economic and social benefits are remarkable.

The invention belongs to the technical field of vegetable cultivation, and particularly relates to a spinach planting method. The spinach planting method comprises steps as follows: 1, determination of sowing time and varieties of autumn spinach: seeds are sown during August and September and can be harvested in batches after being sown for 30-40 days; 2, soil preparation and bedding: loose, fertile and subacid soil with water and nutrient retention functions and good irrigation and drainage conditions is preferably selected, and the pH (potential of hydrogen) value is 5.5-7; 3, sowing and seedling raising: broadcast sowing is generally adopted, the seeds are soaked for 12 hours one week before sowing in summer and autumn, then subjected to germination acceleration at the temperature of 20-25 DEG C and sown after germination in 3-5 days; 4, field management: diluted liquid manure is applied once after autumn spinach grow true leaves, additional fertilizers are applied in the principle that the concentration of the fertilizers is gradually increased in combination of thinning and weeding after two true leaves grow out, and more composted manure fertilizers are applied at the earlier stage; 5, control of diseases and pests: dimethoate which has the concentration of 40% and is diluted for 1000 times or pirimicarb which has the concentration of 50% and is diluted for 2000-3000 times is applied for controlling aphid; 6, timely harvesting: generally, when seedlings are 10 cm high or higher, the spinach can be harvested in batch.

The invention discloses construction and application of a broad spectrum organic phosphorus pesticide degradation genetic engineering strain, and belongs to the field of biological engineering technology. The engineering strain 1gmp is the engineering strain paracoccus sp. 1gmp for degrading the organic phosphorus pesticide in a broad spectrum, which is constructed by sequentially inserting two organic phosphorus hydrolase genes mpd and oph with different substrate degradation spectrums into the chromosome of dimethoate high-efficiency degradation strain paracoccus sp. L3 by using random integration of a serious of vectors pUTTns and deleting a resistant gene by counter-selection marking; and degradation spectrum measurement shows that the engineering strain can efficiently degrade 17 organic phosphorus pesticides generally used in China at present. The engineering strain has the advantages of genetic stability, biological safety and great potential application prospect.

The invention relates to a pest capturing device, in particular to a portable pest capturing device which is combined with a main working cabin and a pest-storing net cabin, etc., which are made from materials such as PVC and arranged with matching negative pressure suction. The device is used for capturing flies, mosquitoes, deerflies and cabbage caterpillars. The device can replace toxic chemical spraying agent such as dichlorvos and dimethoate for killing pests and is applicable to families in urban or suburban areas, guesthouses, restaurants, hotels, hostels, eateries, offices, airplanes, trains, vehicles, ships, dormitories, farms, urban and suburban refuse dumps, environmental hygiene organizations and farmlands all over the world for protecting vegetables, fruits and foodstuff from being contaminated by pesticide residues; the device can effectively capture the pests that harm human beings by spreading cholera, typhoid and destroy farm plants with a speed of capturing 80 to 300 pests per minute, and then distributes the living pests without the chemical contamination of the pesticides to livestock farms for breeding chickens, ducks and fishes as high protein feedstuff so as to realize the aim of stopping general environment pollution, turning harm to benefit and protecting the health of human beings.

The invention provides a bifidobacterium LJM-001 strain. The collection number of the bifidobacterium LJM-001 strain is CGMCC No.4090. The LJM-001 strain is hypersensitive to dimethoate and is a working strain for detecting dimethoate residues in food. The invention also provides a microbiological method for screening dimethoate residues in food. According to the size of antibacterial rings on a screening culture medium, whether the residual quantity of the dimethoate in food samples exceeds the standard or not can be judged. The method for screening the dimethoate residues in food has the characteristics of simpleness and convenience for operation, low cost, high sensitivity and suitability for sample screening, and the screening requirement of a large number of samples in the pesticide residue detection department in China is met.

The invention discloses a liquid chromatography-tandem mass spectrometry method for measuring 18 semi-volatile organic pollutants in water. According to the method, a clean water sample such as surface water, drinking water, and the like is simply filtered by a 0.22 [mu]m micro-porous filter membrane, and then a liquid chromatography-mass spectrometry instrument is used to separate and detect the following 18 compounds in water: aniline, benzidine, acrylamide, picric acid, carbaryl, deltamethrin, microcystic toxin-LR, dibutyl phthalate, bis(2-ethylhexyl) phthalate, dichlorvos, trichlorphon, parathion, methyl parathion, malathion, dimethoate, demeton, atrazine, and carbofuran. The provided method can detect many target substances. The detection range of the method is 0.001 to 0.49 [mu]g / L, the relative standard deviation is 0.7 to 15.4%; and the analysis method has the advantages of high sensitivity, quick analysis speed, little pollution, and simple and efficient pretreatment, is especially suitable for standard analysis of drinking water source monitoring, and solves the problems of bad conformability, large labor strength, and low analysis efficiency of the conventional standard method.

The invention discloses an optimized method for cultivating containerized seedlings of mangrove plant kandelia obovate. The method mainly comprises the following steps: step (1) treatment of kandeliaobovate hypocotyls: soaking the hypocotyls with seawater in situ, then sequentially soaking with a potassium permanganate solution and a dimethoate diluent, and finally washing with tap water; step (2) selection of a seedling container: using a non-woven bag as the seedling container; step (3) preparation of culture medium: preparing the culture medium according to a volume ratio of peat soil to perlite to vermiculite equal to 3: 2: 5; step (4) containerized seedling cultivation method: inserting the hypocotyls into the seedling container containing the medium and placing in a plastic box under a plastic greenhouse to cultivate seedlings; and step (5) seedling management: watering with seawater immediately after the hypocotyls are inserted, and then watering with the tap water once a week.The method has advantages that air root-pruning is adopted to realize sturdy and prosperous seedling roots, the weight is light to facilitate transportation and realize high afforestation efficiency,the seedlings are directly transplanted together with the container to realize less damage to roots, and the non-woven bag can be self-degraded to realize no pollution to environments.

The invention discloses a detection method for organophosphorus pesticides. The detection method comprises the following steps: fully mixing a test sample which may contain organophosphorus pesticides (such as thimet, omethoate and rogor) with gold-core platinum-shell nanoparticles and polyacrylic acid in a phosphate buffer solution so as to form a first mixed system; and then adding a sodium citrate buffer solution and the characteristic substrate of peroxidase, carrying out uniform mixing so as to obtain a second mixed system and determining the light absorption value of the obtained mixture in the range of visible-light wavebands so as to realize detection of the organophosphorus pesticides in the test sample. The invention also discloses a detection kit for the organophosphorus pesticides. The detection kit comprises the characteristic substrate of peroxidase, the gold-core platinum-shell nanoparticles, polyacrylic acid, auxiliary reagents and the like. The detection method for the organophosphorus pesticides in the invention is simple, convenient, rapid, low in cost, high in stability and applicable to detection of residual organophosphorus pesticides in environment samples, food samples and the like.

The invention relates to the field of pest prevention and control in landscape engineering, and in particular relates to a composition for preventing and treating wood-boring pests. The composition consists of the following components in parts by weight: 0.1-20 parts of fenitrothion, 0.1-20 parts of turpentine, 0.1-20 parts of dimethoate, and 1-50 parts of water. The method comprises the following step: thoroughly and uniformly mixing the fenitrothion, the turpentine, the dimethoate and the water according to the formula ration, thus obtaining the composition for preventing and treating wood-boring pests. When the Composition for preventing and controlling wood-boring pests is coated on the branches and trunk or root of a tree, the purpose of preventing the wood-boring pests from damaging the tree can be realized. The Composition for preventing and treating the wood-boring pests has the advantages of high prevention and cure rate, low toxicity, low cost and suitability for popularization in large scale.

The invention provides a disease and pest prevention method for sasanqua, and relates to the technical field of sasanqua planting management. The disease and pest prevention method for the sasanqua is characterized in that the method includes the following steps that sasanqua anthracnose is prevented, from July to September, when the saplings planted for one to two years are in a peak period of diseases, the saplings are sprayed with 1% Bordeaux mixture plus 2% tea bran water three times to four times; leaf eating pests are prevented, phototaxis of adult pests is utilized to trap and kill the pests through lamp light, and 1000-1500-time liquid of dipterex and dichlorvos can be sprayed in the larval phase; branch damaging pests and fruit damaging pests are prevented, feign death of the pests is utilized, manual culling is conducted or 1000-time liquid of 90%-dipterex is sprayed to kill the pests, and 1000-time liquid of 40%-omethoate is sprayed to kill scale insects from March to April. The method is good in prevention effect and not prone to recurring, biological prevention is combined with chemical prevention and thus pollution is smaller.

The invention discloses a fluorescence detection method for organophosphorus pesticide residues, and belongs to the technical field of detection, and the method comprises the following steps: 1), pretreatment of a sample to be tested, to be more specific, washing the sample to be tested, peeling the sample to be tested, cutting into pieces, adding anhydrous methanol, performing ultrasonic treatment, filtering and diluting to obtain filtrate reserve liquid for standby use; 2), drawing of a standard curve, to be more specific, taking dimethoate solutions with different concentrations, respectively adding a Tris-HCl buffer solution, a urease solution, a urea solution and a pyrogallic acid solution for reaction, and adding polymer quantum dots; and measuring fluorescence value of each system,making a standard curve, and calculating the regression equation of the standard curve; and 3) determination of the sample, to be more specific, taking the filtrate reserve liquid obtained in the step1), respectively adding a Tris-HCl buffer solution, a urease reserve solution, a urea reserve solution and a pyrogallic acid solution for reaction, and adding the polymer quantum dots; and measuringfluorescence value of the sample, and substituting the measured fluorescence value into the regression equation of the step 2 to obtain the residue amount of the dimethoate solution in the sample.

The invention discloses peony, acerola cherry and oat powder. The peony, acerola cherry and oat powder consists of the following components of oat flour, purple sweet potato whole powder, coix seed powder, small red bean powder, black bean powder, mung bean powder, black sesame seed powder, black rice flour, acerola cherry fruit powder, cranberry powder, walnut kernel powder, inulin, peony seed oil microcapsule powder, elaeagnus mollis microcapsule powder, fructus perillae oil microcapsule powder, silybum marianum seed oil microcapsule powder, tea seed oil microcapsule powder, corn oligopeptide powder, whey protein powder, Danfeng peony powder, roselle powder, Chinese chestnut powder, rosa roxburghii tratt powder, red jujube powder, borojo patinoi cuatrec powder, lotus root starch, rosa rugosa cv.plena powder, grifola frondosa powder, colla corii asini powder, burdock root powder, milk mineral salts, curcuma powder, collagen peptide powder and the like. The peony, acerola cherry and oat powder disclosed by the invention is full, fine and smooth in mouth feel, comprehensive and harmonious in nutrients, zero in fat, high in fibers, refreshing and capable of removing greasy feeling, can thoroughly remove garbage in intestinal tracts and greasy substances in abdomen, has the efficacy of expelling toxins, nourishing faces, improving constipation, burning fat, eliminating fat, reducing weight, enabling bodies to be slim and enhancing immunity, has great broad market prospects and facilitates popularization and eating.

The invention discloses a treatment method of dimethoate pesticide production wastewater. The problem of difficulty in degradation of high salts, high phosphorus, high nitrogen and the like in the dimethoate pesticide production wastewater can be effectively solved through early-stage air floatation, MVR (short for Mechanical Vapor Recompression), evaporative desalination and dephosphorization, Fe / C micro-electrolysis reaction, coagulating sedimentation, air stripping and other treatment steps, the salt content, the total phosphorus, the total nitrogen and the CODcr value of the wastewater can be effectively reduced, the ratio of BOD5 (biochemical oxygen demand) to CODcr in the wastewater is increased, the biochemical treatment requirements are met, the dimethoate pesticide production wastewater is discharged after being up to standard, and meanwhile, the process is applicable to treatment of other similar industrial wastewater difficult to degrade.

The invention relates to the technical field of microbe application, particularly to an aspergillus niger JXJ A01 with potassium and phosphate dissolving and organophosphorus pesticide decomposing activity and application of the aspergillus niger JXJ A01 with the potassium and phosphate dissolving and organophosphorus pesticide decomposing activity to dissolution of insoluble potassium, organophosphorus and inorganic phosphorous, decomposition of organophosphorus pesticides and preparation of microbial fertilizers. The aspergillus niger JXJ A01 is stored in China General Microbiological Culture Collection Center with a preservation number of CGMCC No. 13775. The aspergillus niger JXJ A01 is high in dissolving effects on insoluble phosphorous and potassium ore powder and can convert the insoluble phosphorous and the potassium ore fertilizer into soluble phosphorous and potassium absorbable for plants and degrade the organophosphorus pesticide of omethoate into non-toxic soluble phosphate radicals to reduce pesticide concentration and increase the content of soluble phosphorous in soil. The aspergillus niger JXJ A01 with the potassium and phosphate dissolving and organophosphorus pesticide decomposing activity can be applied to increasing the content of soluble effective potassium and phosphorus in soil and repair pollution caused by the organophosphorus pesticides to soil.

The invention discloses a fluorescent CdSe nanometer quantum dot probe for pesticide dimethoate, and application of the probe. A preparation method of the fluorescent CdSe nanometer quantum dot probe for pesticide dimethoate comprises the steps of preparing selenium stock solution; preparing cadmium stock solution; and preparing the fluorescent CdSe nanometer quantum dot probe for pesticide dimethoate. The probe has detection limit up to 1.5*10<-7> mol / L for pesticide dimethoate. The preparation method has the advantages of simple operation method, rapid detection, and high sensitivity and selectivity for dimethoate in a mixed sample.

The invention relates to a wastewater treatment process of a dimethoate pesticide, in particular to a wastewater treatment method of the dimethoate pesticide, and belongs to the technical field of chemistry. Wastewater of the dimethoate pesticide is subjected to photo-assisted Fenton oxidation treatment for 15 to 120 minutes (min), effluent is diluted to reach twofold volume after a reaction is finished, and the diluted effluent is subjected to biological treatment for 5 to 8 hours (h) through a cyclic activated sludge process (a CAST process for short); the wastewater of the dimethoate pesticide is only subjected to Fenton process pretreatment and the CAST process, so the wastewater treatment process is superior to a method which is used for treating the wastewater by combining three processes of photo-assisted Fenton, flocculation and micro-biological degradation and is reported in an invention publication (CN1636983A); the optimum time required by a Fenton pre-reaction process does not exceed 0.5 hour, and compared with that of the conventional method, the pretreatment time is shortened greatly and is superior to time required by various pretreatment processes in a document, namely 'the summarization of a pretreatment method of organophosphorus pesticide wastewater'; and the final CODcr removal rate of the wastewater of the dimethoate pesticide can reach 95 percent.

The invention discloses bacillus subtilis BLCC1-0388, which is collected in China Center for Type Culture Collection, and the collection number of the bacillus subtilis is CCTCC NO:M 2015503. The invention also discloses application of the bacillus subtilis in degrading organophosphorus pesticide and a product taking the bacillus subtilis as an effective component. The bacillus subtilis disclosed by the invention has a better degradation effect on the organophosphorus pesticide, especially has a remarkable degradation effect on omethoate, and has a broad application prospect in sustainable agricultural development; moreover, the strain of the bacillus subtilis disclosed by the invention can be planted and bred in soil under the condition that only the soil exists, and is capable of degrade the omethoate.

The invention relates to a pesticide for plants and belongs to the technical field of pesticide. The common chemical agents have different-degree damages to vegetables and fruits, for example, omethoate cannot be used in 30 days before harvesting and malathion can produce damage to melons and peaches. The invention provides a nontoxic and nuisanceless pesticide for plants. The pesticide for plants is characterized by comprising 4-6 parts by mass of purslane extract, 4-6 parts by mass of pasque flower extract, 3-8 parts by mass of celandine extract and 2-5 parts by mass of chitin.

The invention discloses a method for naked eye discernible colorimetric detection on dimethoate. The method comprises the following steps: A, drawing a dimethoate absorbancy variation standard curve;B, according to the dimethoate absorbancy variation standard curve, establishing a linear regression equation according to the mutual relationship between dimethoate concentration and dimethoate absorbancy values; C, preparing a solution C to be detected with unknown dimethoate concentration; D, performing absorption spectrum scanning on the solution C, testing the absorbancy value of the solutionC at the wavelength of 450nm, calculating an absorbancy value difference of the solution C and a product A, and substituting the absorbancy value difference into the linear regression equation, thereby obtaining the concentration of dimethoate in the solution C. The method needs no large-size instrument equipment in the application process, has the advantages of high detection sensitivity, good selectivity, simple and rapid operation, naked eye discrimination and the like and can be widely applied to rapid detection on dimethoate residues in agricultural products.

The invention discloses a high-yield cultivation method for stingless wild peppers. The high-yield cultivation method comprises the following steps of (1) seedling nursery land selection, wherein a sheltered sunny slope, a sheltered semi-sunny slope or a sheltered ridge edge is selected, the land with abundant sunlight and deep and fertile soil layers is selected, the gradient is from 15 degrees to 30 degrees, and the land is built into a terraced field or prepared; (2) seed collection, wherein plump fruits fully matured are collected from a seed tree which is excellent in variety and free from diseases and insect pests and grows strongly, and then seeds are taken out from the fruits after the fruits are dried in the shade; (3) seedling cultivation, wherein a ditching and strip sowing mode is adopted, sowing is carried out according to the line spacing of 20-25 cm, the planting distance of 10-15 cm, and the ditch depth of 3-5 cm, and the planting number is 675-1650 plants / hm<2>; (4) fertilizer application, wherein 5-10 kg of farm manure, 0.5-0.15 kg of urea and 0.1-0.2 kg of phosphate fertilizer are applied for each seedling; (5) pest control, wherein in the second half of May and June, 2.5% of deltamethrin cream in 4000-fold liquid and omethoate in 2000-fold liquid are respectively used for spraying a crown once; (6) harvesting, wherein five to seven years after planting, harvesting is carried out in batches according to the maturity. According to the method, the yield of the stingless wild pepper is increased, and the high-quality stingless wild pepper can be produced.

The present invention relates to pesticide technology, and is recombinant acetylcholinesterase and its preparation process and usage in detecting pesticide residue. Recombinant acetylcholinesterase may be dissolved in buffering sodium phosphate solution and after being maintained at 4 deg.c for 22 days, it has 75 % of its initial enzyme activity maintained. It has inhibiting constant on DDVP, omethoate, paraoxon, methyl paraoxon, aldicarb, carbaryl, carbofuran and aphox of 4.4E6, 2.2E4, 1.1E6, 4.2E5, 4.8E4, 5.9E5, 5.1E6 and 2.3E5 separately. It is prepared through selecting seed, shaking cultivation, centrifuging, filtering, depositing, leaching, stirring, dialyzing and freeze drying, and is maintained at -20 deg.c. The detection process includes preparing reagent, treating sample, detecting sample, blank testing, testing sample, and final calculating. The recombinant acetylcholinesterase has obvious sensitivity on organophosphorus pesticide and carbamate chemicals and may be used in detecting pesticide residue.

The invention relates to a method for rapidly and quantitatively detecting omethoate residue. According to the present invention, the characteristic that the omethoate aqueous solution presents the stable absorption peak at the specific ultraviolet wavelength is utilized to establish the method, wherein the omethoate content is proportional to the absorbance value and the proportional change trend is presented in the coordinate system so as to establish the standard curve, and the detected absorbance mean value of the detected sample is substituted into the regression equation so as to calculate the omethoate content in the sample, such that the purpose of the rapid and quantitative omethoate residue detection is achieved.