The invention relates to an
ionic liquid regulation-control bio-
enzyme, a method for degrading environmental
hormone carbaryl by the same, and a method for determining the degradation rate. The
ionic liquid regulation-control bio-
enzyme comprises the
mixed solution formed by adding chloroperoxidase,
quaternary ammonium salt ionic liquids and H2O2 in a
phosphate buffer solution with a pH value of 2-5, wherein in the
mixed solution, the concentration of chloroperoxidase is 0.005-0.05mu mol.L<-1>, the concentration of
ammonium salt ionic liquids is 0.06-0.20mol.L<-1>, and the concentration of H2O2 is 0.05-0.5mmol.L<-1>. According to the
ionic liquid regulation-control bio-
enzyme, the method for degrading environmental
hormone carbaryl by the same, and the method for determining the degradation rate disclosed by the invention, the chloroperoxidase is used as a biocatalyst, the
ammonium salt ionic liquids are used as regulation-control factors, the ammonium salt ionic liquids have a cosolvent effect on the chloroperoxidase, and can be used for improving the stability of the chloroperoxidase, increasing the
enzyme catalysis speed and increasing the effective
utilization rate of the chloroperoxidase; the ionic liquid regulation-control bio-enzyme is capable of effectively improving the
solubility of
carbaryl and increasing the degradation rate in case of being applied to degradation for carbaryl.