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Method for screening compounds having activity of protecting liver function of zebra fishes

A technology for zebrafish and liver function, which is applied in the directions of organic active ingredients, medical preparations containing active ingredients, and pharmaceutical formulations, can solve the problems of excessive differences in evaluation results, errors in the screening model of liver-protecting drugs, and inability to be practically applied. Achieve the effect of reducing experimental cost, stable and reliable repeatability, and reducing production cost

Active Publication Date: 2015-12-23
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the damage degree of the liver function injury model and the evaluation standard of the corresponding hepatoprotective drug screening model have large errors and the evaluation results are too different to be practically applied.

Method used

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  • Method for screening compounds having activity of protecting liver function of zebra fishes
  • Method for screening compounds having activity of protecting liver function of zebra fishes
  • Method for screening compounds having activity of protecting liver function of zebra fishes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1: Establishment method of zebrafish liver function injury model

[0065] 1. Acquisition and Use of Juvenile Zebrafish

[0066] Use healthy and sexually mature zebrafish whose liver specifically expresses fluorescence, put them in a mating tank at a ratio of 1 / 1 or 1 / 2, place a partition in the middle, and place it in a dark environment. Remove the partition before turning on the light the next day , stimulated by light to ovulate, fish out the adult fish half an hour later, and control the ovulation time within half an hour to reduce the difference in development time between embryos. Fertilized eggs were collected, sterilized and cleaned, then transferred into zebrafish embryo culture water, and 0.2ppm methylene blue was added to the culture water, and light-controlled culture was performed at 28°C. Add 0.2mM phenylthiourea 12 hours after the birth of the zebrafish embryos, change about 1 / 2 of the water every 24 hours in the middle, and suck out the dead embr...

Embodiment 2

[0083] Example 2: Protective effect of reduced glutathione on zebrafish liver damage caused by carbaryl

[0084] 1. Acquisition and Use of Juvenile Zebrafish

[0085] Use healthy and sexually mature zebrafish whose liver specifically expresses fluorescence, put them in a mating tank at a ratio of 1 / 1 or 1 / 2, place a partition in the middle, and place it in a dark environment. Remove the partition before turning on the light the next day , stimulated by light to ovulate, fish out the adult fish half an hour later, and control the ovulation time within half an hour to reduce the difference in development time between embryos. Fertilized eggs were collected, sterilized and cleaned, then transferred into zebrafish embryo culture water, 0.2 ppm methylene blue was added to the culture water, and light-controlled culture was carried out at 28°C. Add 0.2 mM phenylthiourea 12 hours after the birth of zebrafish embryos, change about 1 / 2 of the water every 24 hours, and suck out dead em...

Embodiment 3

[0094] Example 3: Protective effect of vitamin C on zebrafish liver damage caused by carbaryl

[0095] 1. Acquisition and Use of Juvenile Zebrafish

[0096] Use healthy and sexually mature zebrafish whose liver specifically expresses fluorescence, put them in a mating tank at a ratio of 1 / 1 or 1 / 2, place a partition in the middle, and place it in a dark environment. Remove the partition before turning on the light the next day , stimulated by light to ovulate, fish out the adult fish half an hour later, and control the ovulation time within half an hour to reduce the difference in development time between embryos. Fertilized eggs were collected, sterilized and cleaned, then transferred into zebrafish embryo culture water, and 0.2ppm methylene blue was added to the culture water, and light-controlled culture was performed at 28°C. Add 0.2 mM phenylthiourea 12 hours after the birth of zebrafish embryos, change about 1 / 2 of the water every 24 hours, and suck out dead embryos in ...

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Abstract

The invention relates to a method for screening compounds having activity of protecting liver function of zebra fishes. The method includes: enabling carbaryl and a to-be-detected compound to jointly act on a zebra fish, or using carbaryl to act on the zebra fish to cause liver damage and then using the to-be-detected compound to treat the zebra fish after being damaged; utilizing liver area index change as an indicator to detect the liver function, and analyzing whether the to-be-detected compound has the activity of protecting or improving the liver function or not. For the first time, carbaryl is utilized to build a zebra fish liver function damage model, and the zebra fish liver function damage model has the advantages of simplicity and quickness in building, stability, reliability and high repeatability, so that building cost of the liver function damage model is lowered, and reliability of experimental study results is improved.

Description

technical field [0001] The present invention relates to a method for screening active compounds capable of protecting zebrafish liver function, in particular to a method for using carbaryl to prepare a zebrafish liver function injury model and using it to screen active compounds capable of protecting zebrafish liver function, belonging to drug screening technology field. Background technique [0002] The liver is the main organ for drug concentration, transformation, and metabolism. Especially, oral drugs enter the liver after being absorbed from the gastrointestinal tract. The concentration of drugs in the liver is higher than that in the blood and other organs. The liver is extremely vulnerable to damage due to the toxic effects of drugs or their metabolites, or from an allergic reaction to drugs. Many drugs are known to cause liver damage, such as anti-tumor chemotherapy drugs, anti-tuberculosis drugs, antipyretic and analgesic drugs, immunosuppressants, hypoglycemic and...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K31/222
Inventor 张云刘可春韩利文何秋霞王雪彭维兵陈维云王希敏侯海荣陈锡强
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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