357 results about "Luminol" patented technology

A firearm ammunition device includes a cartridge with a projectile that is loaded with a tracer agent. When the projectile is fired and strikes a game animal, the impact causes the release and dispersal of the tracer agent from the projectile. The dispersed tracer agent identifies an enhanced-visibility trail of the fleeing animal. In example embodiments, the cartridge is provided by a centerfire cartridge, a shotgun cartridge, and a muzzleloading cartridge. The tracer agent preferably comprises one or more compounds that produce visible light to the naked eye and / or under a black light source. Example tracer agents include biological stains that produce visible light upon contact with blood, luminol or another chemiluminescent compound that releases light by a chemical reaction such as may occur when contacting blood, a basic salt such as sodium carbonate or another effervescence-inducing agent, a phosphorescent compound, or a fluorescent compound.

A firearm ammunition device includes a cartridge with a projectile that is loaded with a tracer agent. When the projectile is fired and strikes a game animal, the impact causes the release and dispersal of the tracer agent from the projectile. The dispersed tracer agent identifies an enhanced-visibility trail of the fleeing animal. In example embodiments, the cartridge is provided by a centerfire cartridge, a shotgun cartridge, and a muzzleloading cartridge. The tracer agent preferably comprises one or more compounds that produce visible light to the naked eye and / or under a black light source. Example tracer agents include biological stains that produce visible light upon contact with blood, luminol or another chemiluminescent compound that releases light by a chemical reaction such as may occur when contacting blood, a basic salt such as sodium carbonate or another effervescence-inducing agent, a phosphorescent compound, or a fluorescent compound.

A compact scanning apparatus has an infrared laser adapted to emit light. The light is delivered as a beam by an optical system to illuminate an interrogation area on the surface of an object being scanned. Such illumination has sufficient intensity and duration to cause selective desorption of molecules of the contraband substance, which are present on the surface, without substantially damaging the surface. A collection system collects at least a portion of the desorbed molecules. At least a portion of the collected molecules is thermally decomposed to form NO2 and transferred to a reaction cell containing an aqueous, alkaline, luminol-containing solution. The NO2 reacts with the luminol to produce light by chemiluminescence. A light detector registers the presence of this light as indicative of the detection of the contraband substance, and activates a signaling device to provide an audible or visible alarm. The apparatus rapidly detects the presence of a wide variety of contraband substances in an accurate, reliable manner. It provides for automated screening, with the result that vagaries of human performance are virtually eliminated. False alarms are reduced and detection efficacy is increased. A traceable residue of the detected contraband is left on the article for use in forensic analysis.

A multi-zonal system and method for detecting contraband substances associated with a human subject. Objects containing such substances may be carried by the subject or in a garment worn by the subject, or as vapors entrained by the subject's garments. Alternatively, the substance may be present as small particles or residues present on skin, garment fibers, or the like that may be dislodged from the subject or his immediate effects. The system comprises: (i) an examination station through which the subject passes; (ii) sample generation means to produce a sample for analysis, comprising a plurality of air jets disposed in the examination station to impinge flowing gas onto pre-selected zones on the subject; (iii) collection means operative to acquire and convey the sample, comprising a plurality of collection ports disposed in the examination station to receive gas deflected by the subject from the air jets; (iv) analysis means in communication with the collection means to receive the sample and carry out a chemical analysis to detect the contraband substances; and (v) signal means operably connected to the analysis means for indicating detection of the contraband substances. Preferably the system employs a chemiluminescent detector based on the reaction of luminol with NO2 produced by pyrolysis of explosive or other contraband substance and is capable of high throughput, accurate screening in real time of a large number of subjects, e.g. persons being screened in an airport.

A compact scanning apparatus has an infrared laser adapted to emit light. The light is delivered as a beam by an optical system to illuminate an interrogation area on the surface of an object being scanned to cause selective desorption of molecules of the contraband substance, which are present on the surface, without substantially damaging the surface. A collection system collects at least a portion of the desorbed molecules. At least a portion of the collected molecules is thermally decomposed to form NO2 and transferred to a reaction cell containing an aqueous, alkaline, luminol-containing solution. The NO2 reacts with the luminol to produce light by chemiluminescence. A light detector registers the presence of this light to carry out a rapid screening of the object for the possible presence of the contraband substance. The apparatus further includes a supplemental detector such as a GC / IMS detector that is activated in response to the detection of the chemiluminescent light. The supplemental detector provides confirmation of the detection of contraband substance and activates a signaling device to provide an audible or visible alarm. The rapid pre-screening permits the apparatus to identify suspicious items, while the supplemental detection system can be optimized for more intense, but time-consuming scrutiny of just the suspicious items. Both effective detection and high throughput are thereby achieved in an accurate, reliable manner.

The invention discloses an application of nano-gold directly bonded with luminol in an immunoassay. The invention is characterized in that the immunoassay probe of a nano-gold directly bonded with luminol comprises antibodies which are labeled by the nano-golds directly bonded with luminol, wherein the nano-gold directly bonded with luminol are prepared through reducing chloroauric acid by the luminol at a single step; a chemiluminescence immunoassay method is based on the immunoassay probe of the nano-gold directly bonded with luminol; and a kit is used for carrying out the immunoassay method. The chemiluminescence immunoassay method of the invention has the advantages of high sensitivity (for instance, the detection limit can reach 1.0pg / mL for detecting human IgG), wide range of linearity, good repeatability, simple operation, low cost and the like, can be applied to detect multi-analyte in various samples and has key application prospect in fields, such as clinical diagnosis and cure, drug analysis, food security detection, environmental monitoring and the like.

The invention provides preparation and application of an alpha fetoprotein and carcino-embryonic antigen electrochemiluminescence sensor, and belongs to the technical fields of nano function material, clinical analysis, a bioseneor technology and electrochemistry. The characteristics that platinum nanoparticle @meso-porous silicon @ graphene nanocomposite (PtNPs@m-Si@GS) is strong in conductivity, good in stability, large in specific surface area, good in biocompatibility, strong in catalytic activity and the like are utilized in preparation; an alpha fetoprotein second antibody (anti-alpha fetoprotein (AFP)) and a carcino-embryonic antigen secondary antibody (anti-carcino-embryonicantigen (CEA)) are marked, so as to prepare the marked second antibodies Ru-PtNPs@M-Si@GS / anti-AFP and luminol-PtNPs@M-Si@GS / anti-CEA; and the sensitivity of the sensor is obviously improved. Compared with other single-channel electrode sensors, alpha fetoprotein and carcino-embryonic antigen can be simultaneously detected on a same electrode at one time; the detection efficiency is obviously improved; and the alpha fetoprotein and carcino-embryonic antigen electrochemiluminescence sensor has important scientific significance and application value on clinical early diagnosis of hepatic carcinoma.

The invention discloses a stable HRP enzymatic enhanced chemiluminescent substrate solution. The stable enzymatic enhanced chemiluminescent substrate solution is composed of a luminescent agent solution A and an oxidizing agent solution B, which are mixed in volume ratios of 2:3-3:2. The luminescent agent solution A contains a luminescent agent: luminol or luminol derivatives, and a reinforcing agent: p-iodo-pheno, 4-(1, 2, 4-triazole-1-radical) phenol, etc., and the oxidizing agent solution B contains sodium perborate, hydrogen peroxide, etc. The stable HRP enzymatic enhanced chemiluminescent substrate solution of the invention is applied in an HRP enzymatic reaction with the characteristics of short reaction time and long platform period, can be stably preserved for 2 years at 4-8 EDG C, and is widely used in a kit using HRP as the detection object.

The invention discloses a preparation method of an unmarked electrochemiluminescent tumor marker immunosensor and application of the immunosensor in detection of tumor markers, and belongs to the field of novel nano functional materials and biosensors. According to the preparation method of the unmarked electrochemiluminescent tumor marker immunosensor, a novel micro-nano material NH4CoPO4 is taken as a substrate so that the luminescence stability of a luminol-hydrogen peroxide luminescent system can be greatly enhanced, and meanwhile, rod-like gold@siver core-shell nanoparticles are taken as a biomimetic catalytic luminescent system, and therefore, an unmarked electrochemiluminescent immunosensor, which is low in cost, high in sensitivity, good in specificity, fast in detection and simple to prepare and is used for detecting tumor makers, can be prepared.

The invention discloses a luminol direct bonded nano gold nucleic acid analyzing probe and a novel chemiluminescence analysis method. The invention provides the luminol direct bonded nano gold nucleic acid analyzing probe, a chemiluminescence analysis method based on the luminol direct bonded nano gold nucleic acid analyzing probe and a kit for constructing the analyzing method; the analyzing probe comprises nucleic acid which is directly bonded by luminol and marked by nano gold; and luminol direct bonded nano gold is obtained by reducing chloroauric acid at one step by luminol. The chemiluminescence analysis method based on the nucleic acid analyzing probe of the invention has the advantages of high sensitivity (for example, specific sequence single-chain DNA detection limit can reach 1.9*10<-16>mol / L), wide linear range, high repeatability, simple operation, low cost, and the like, can be used for the detection of DNA, RNA and adapter corresponding ligands in various samples and has wide application prospect in the fields of clinical diagnosis and treatment, pharmaceutical analysis, food safety detection, environmental monitoring, and the like.

The invention discloses a luminol-luminescent functionalized nano-silver as well as a preparation method and an application for the same. The invention discloses a functionalized nano-silver, formed by connecting silver nanoparticles with luminol, wherein the luminol is connected to the surfaces of the silver nanoparticles via a covalent bond Ag-N. In the preparation method, nano-silver is prepared by directly reducing silver nitrate by luminol in the presence of ethanol, and luminol is used as a reducing agent and a protecting reagent simultaneously during synthesis process. The method has the advantages of being simple, fast, cheap and the like, as well as is a new technology for synthesising nano-silver at a temperature ranging from 25 to 40 DEG C. The particle size and shape of the nano-silver synthesised by the method can be regulated and controlled by the ratio of silver nitrate to luminol, and the obtained nano-silver has excellent chemiluminescent characteristic. The invention further discloses a bioanalysis probe based on the luminescent functionalized nano-silver, as well as an analysis method based on the probe and a kit. The functionalized nano-silver, the bioanalysis probe thereof, the analysis method and the kit disclosed by the invention can be used in the fields of immunoassay, nucleic acid analysis, molecular imaging, sensor etc.

The invention relates to the encapsulation of luminescence-related molecules, including but not limited to, adenosine triphosphate (ATP), adenylate kinase (AK), alkaline phosphatase (ALP), luminol and luciferin / luciferase cocktails, within liposomes. These liposomes can be employed to enhance the luminescence detection of microorganisms and compounds in various products and samples. The liposomes containing the luminescence-related molecules can bear a probe which has a specific sequence or structure that, in turn can be used to hybridize to, or couple with, a portion of the target analyte. Within the same assay, paramagnetic beads can bear a probe having a specific sequence or structure that, can hybridize to, or couple with, a second portion of the target analyte to create a complex of analyte bound to paramagnetic beads and liposomes. This type of assay can be often referred to as a ‘sandwich’ assay. Once the probes hybridize to, or couple with, their targets, a complex can be formed of the paramagnetic beads, the analyte, or portion thereof, and the liposomes. This complex can then be washed to remove those components that are non-hybridized or non-coupled. Then, the paramagnetic bead-analyte-liposome complexes can be isolated from the sample using magnetic separation techniques and can be treated so as to release their encapsulated ATP, AK or other luminescence-related compounds. The resulting luminescence can then be determined in a chemical assay. This determination can be qualitative (i.e., an absence / presence assay) or quantitative (i.e., which can measure a specific amount of analyte present). Through the use of a cocktail of probe types, the assay can also qualitatively or quantitatively measure the presence of more than one analyte simultaneously. This type of assay can be of commercial importance in clinical and forensic applications, the personal care, pharmaceutical, food and beverage markets, as well as in environmental sample assays.

The invention belongs to the field of chemiluminescence sensors, and relates to a method for determining melamine (Me), which is implemented by using a target induced chain releasing technique and combining with a hybrid chain reaction and a chemiluminescence technique. When a target Me exists, the Me acts with aptamer DNA1 (deoxyribonucleic acid 1) in double-stranded DNA on the surface of a functionalized magnetic bead (FMB1) so as to release a complementary sequence DNA2 of the aptamer; and the released complementary sequence DNA2 acts with another functionalized magnetic bead (FMB2), and a chain hybridization reaction is performed in the presence of hairpin DNA (HRP-H1 and HRP-H2) for modifying HRP, so that the two kinds of modified DNA grow into long double-stranded DNA. Through magnetic separation, and by using the catalytic action of HRP on a luminol-H2O2 chemiluminescence system, chemiluminescence is produced, and then the determination of the Me is realized through determining the chemiluminescence intensity. The method is high in selectivity and detection sensitivity.

The invention relates to a diagnostic reagent kit for testing the hepatitis c virus (HCV) and the preparation and test method, which is to add the HCV recombinant antigen used for peridium into the buffer solution, blend it, move into the luminous microplate, make incubation for 18 hours under 4DEG.C, wash the luminous microplate, add into the confining liquid, leave the liquid after incubation and fully dry the luminous microplate to complete the preparation of the pre-peridium luminous microplate; combine the anti-human IgG used for marking and the horse radish peroxidase by improving the sodium periodate to complete the preparation of the enzyme marker; prepare the chemical luminous substrate solution A with luminal, Tween20 and luminous intensifier and prepare the chemical luminous substrate solution B with the hydrogen peroxide. The reagent kit also comprises the sample diluent and concentrated scrub solution. The negative corresponds to the normal human serum while the positive corresponds to the people with serum of pooled serum with HCV antibody. The reagent kit provided in the invention has much higher detection sensitivity than the ELISA, which is safe and reliable, easy to operate with low cost, and without any expensive full-automatic chemical luminous measuring apparatus required.

The invention discloses a luminescent substrate, a use of the luminescent substrate and a detection kit containing the luminescent substrate. The luminescent substrate has high sensitivity and high stability. The luminescent substrate comprises a solution A and a solution B. The solution A comprises 0.5-8mmol / L of luminal, 0.5-10mmol / L of p-lodophenol, and 0.1mol / L of a Tris-HCl buffer solution. The solution B comprises 1-10mmol / L of urea peroxide and 0.1mol / L of the Tris-HCl buffer solution. The kit comprises a monoclonal antibody-coated microporous plate, HRP-labeled monoclonal antibodies, a freeze-dried standard, a sample diluent, a concentrated washing liquid and the chemiluminescent substrate.

Method for increasing and regulating the emission of light from a chemiluminescent reaction including luminol, a peroxidase enzyme, an oxidant and an electron mediator (primary enhancer) through the use of an acylation catalyst (secondary enhancer) belonging to the class of N-azoles, i.e., a class of five-membered nitrogen heteroaromatic ring compounds containing at least one other atom of nitrogen. N-azoles, which are especially useful as secondary enhancers are imidazole, 1-methylimidazole, 1,2,3-triazole and 1,2,4-triazole. The invention also describes the use in diagnostic assays of chemiluminescent substrates containing said N-azoles, as secondary enhancers.

The invention provides a method for detecting protease activity and inhibitor activity thereof based on chemiluminescence, and relates to the technical field of biological analytical chemistry. The method for detecting the protease activity and the inhibitor activity thereof based on the chemiluminescence solves the problems that an existing method for detecting the enzyme activity is complex in flow design, complicated in operation, low in detection sensitivity, and high in cost. The method for detecting the protease activity and the inhibitor activity thereof based on the chemiluminescence comprises a first step of dissolving heme proteins into Tris - HCl buffer solution to achieve heme protein solution, a second step of adding protease with different concentrations into the heme protein solution of the first step to form a reaction system, and a third step of injecting the reaction system of the second step into a flow-injected chemiluminescence flow, and reacting a chemiluminescence reaction with a luminol hydrogen peroxide detecting system so as to detect the protease activity. The method for detecting the protease activity and the inhibitor activity thereof based on the chemiluminescence has the advantages of being simple, quick and high in sensitivity, low in cost, and low in detection limit, and the detection limit of trypsin can achieve to 1 ng / mL.

A bowhunting tracking-assist device on an arrow's shaft includes a tracer agent and an agent dispenser. The dispenser releases the agent when the arrow strikes a game animal, and the released agent identifies an enhanced-visibility trail of the fleeing animal. In an example embodiment, the dispenser has wells for holding the agent and a cover that displaces to release the agent when the arrow drives into the animal. The tracer agent preferably comprises one or more compounds that produce visible light to the naked eye and / or under a black light source. Example tracer agents include biological stains that produce visible light upon contact with blood, luminol or another chemiluminescent compound that releases light by a chemical reaction such as may occur when contacting blood, a basic salt such as sodium carbonate or another effervescence-inducing agent, a phosphorescent compound, or a fluorescent compound.

The invention is a biochemical sensing device, including a photodiode capable of sensing the light generated by the reaction made by a specific compound, a specific enzyme, and a luminol as well as converting the optical signal into a current signal. Also, there is a current / voltage converting circuit capable of converting the current signal into an analog voltage signal. In turn, the analog voltage signal can be converted into a digital voltage signal through an analog / digital converter. Finally, by using an electronic device, the digital voltage signal can be received and analyzed, and through the analysis, the amount of the specific compound can be measured. The device of the invention can provide a simple real-time medical assay that can be performed in massive amount. For this reason, the drawbacks of a conventional spectrum analysis instrument of being bulky and expensive can be improved.

The invention relates to a kit for rapidly detecting organophosphorus pesticide residues and an application method thereof. The kit comprises a C enzyme bottle, a T enzyme bottle, a C substrate reaction cup and a T substrate reaction cup, wherein the C enzyme bottle and the T enzyme bottle are sealed by using enzyme bottle sealing covers and are filled with an enzyme lyophilized three-enzyme mixing agent; the C substrate reaction cup and the T substrate reaction cup are provided with lyophilization and water-absorption cushions containing acetyl choline, luminol and dimethylbenzidine. The invention further relates to a method for applying the kit to rapidly detecting the organophosphorus pesticide residues. The kit takes acetylcholin esterase, choline oxidase and peroxidase as a catalysis system; a product obtained by hydrolyzing and oxidizing acetylcholine chloride and luminol have a chemiluminescence effect. A method for flexibly testing organophosphorus pesticides in agricultural products is established. The kit has the advantages of high detection speed, simple operation process, good stability and repeatability, no need of expensive instruments and the like, and is very suitable for carrying out field monitoring on the organophosphorus pesticides in farm markets.

The invention relates to a device and method for measuring the organophosphorus pesticide residues by chemiluminescence, which comprises an analyzer and a photolysis device connected with each other through conduits. The method dissociates the organophosphorus pesticide residues on the vegetables or fruits to phosphates by the potassium peroxodisulfate catalyzed with the titania nanometer particle and ultraviolet light. The phosphates and molybdates and vanadates form phosphomolybdic vanadium acid, which oxygenizes the luminol liquor and causes chemiluminescence, then definites quantity according to the intensity and measures the total quantity of residues. The measuring linear range is 1.0x10-9-1.0x10-5g / ml, detection limit is 8.0x10-10g / ml, relative standard deviation is 4.8%.

The invention provides a chemiluminescent substrate solution, which comprises the following components: 0.2-1.2g / L of Luminol, 0.1-5g / L of cinnamic acid, 0.05-0.6g / L of 4-iodophenylboronic acid, 0.05-0.6 g / L of p-iodophenol, 5-50ml / L of dimethylformamide (DMF), 2.5-7.5 g / L of polyvinyl alcohol (PVA), 5-15g / L of polyvinyl pyrrolidone (PVP), 1.5-5g / L of polyethylene glycol 600 (PEC -600), 1-10g / L of ethylene diamine tetraacetic acid (EDTA), 400,000-4,000,000 unit / L of gentamicin sulfate, 0.1-1.0g / L of carbamide peroxide and 0.1mol / L of tris buffer solution with a pH vale being 9.0. The chemiluminescent substrate solution is a solution which can be directly used, and maintains wide linear detection range, good stability and high sensitivity, thus solving the problem that a traditional chemiluminescent substrate solution consists of a solution A and a solution B which are stored separately; and after the solution A and the solution B are isovolumetrically mixed for 10 minutes, signal values are decreased sharply, the detection range is narrowed and the stability is poor. In addition, the chemiluminescent substrate solution provided by the invention is easy to prepare, low in cost and simple to operate, environmentally friendly, and non-toxic and harmless to human bodies and the like.

The invention discloses a method for detecting glucose by ferroferric oxide nano particle catalytic chemiluminescence, which comprises the following steps of: at the temperature of between 10 DEG C and 60 DEG C, adding a glucose solution into a ferroferric oxide nano granule-luminol-H2O2 solution system, the pH of which is 4.0 to 9.0; and detecting the concentration of the glucose by using a SpectraMaxM2 microplate reader, wherein the concentration of the ferroferric oxide nano particles is more than or equal to 10ppm. The average granule diameter of the ferroferric oxide nano particles is 25nanometers. By using the characteristics of high adsorption, biocompatibility, zymoid catalytic property and reuse of the ferroferric oxide nano particles, the glucose chemiluminescence detection method with simple operation, low cost, wide linear range and low detection limit is established to realize simple, convenient and high-sensitivity detection of the glucose content in the fields of clinic and food.

The invention relates to preparation of a chemiluminiscence light-induced electrochemical sensor and organic phosphorus pesticide detection. Preparation is achieved through the steps that gold nanoparticles are electrochemically deposited on conductive glass; zinc oxide nanorods are prepared on the gold nanoparticles deposited on the conductive glass through a seed growing mode; CdS electrochemical deposition is conducted in an electro-deposition solution prepared from cadmium nitrate, thiourea, dimethylsulfoxide and water to obtain CdS quantum dots loaded on the ZnO nanorod surfaces; a molecularly-imprinted polymer film loaded on the surface of an electrode is prepared by taking an organic phosphorus pesticide as a template molecule, taking methacrylic acid as a functional monomer, taking dimethylacrylicacid glycol ester as a cross-linking agent, taking acetonitrile as a pore-forming agent and taking azodiisobutyronitrile as an initiating agent; a chemiluminescent reagent N-(4-ammonia butyl)-N-ethylisoluminol is covalently connected to the molecularly-imprinted film; the template molecule in the molecularly-imprinted polymer is eluted with methanol and glacial acetic acid, and then the chemiluminiscence light-induced electrochemical sensor is prepared. Chemiluminiscence generated by hydrogen peroxide and N-(4-ammonia butyl)-N-ethylisoluminol is taken as a light source, ZnO and CdS are excited by light to generate light currents, and the sensor can be applied to organic phosphorus pesticide detection.

The invention discloses a chemoluminescent substrate, which can be applied in the field of immunological detections, and comprises luminescent agent solution and oxidant solution, wherein the luminescence agent solution contains reinforcers, namely 4-hydroxydiphenyl and 4-iodo-borophenylic acid, a luminescent agent, namely luminal or luminal derivatives and buffer solution. The chemoluminescent substrate has high stability, good luminescence effect, long luminescent time, high sensitivity and low cost.

The invention relates to a high sensitive and jettisonable multicomponent chemiluminescent imaging immunosensor. The immunosensor is prepared by: constructing a 4*12 array on a silanized glass slide by a screen printing technique, and coating the array points with different capture antibodies to construct a jettisonable multicomponent immune sensor array; immobilizing biotinylated capture DNA and multiple G-quadruplex sequence repeat signal DNA on gold nanoparticle surfaces simultaneously, combining G-quadruplex signal DNA with heme to form DNA enzyme so as to prepare a multilayer DNA enzyme functionalized gold nanoparticle probe; and based on sandwich immunoassay, forming a sandwich immune complex on the sensor array, carrying out biotin-avidin reaction, labeling different immune complexes with the multilayer DNA enzyme functionalized gold nanoparticle probe; and making use of the peroxidase characteristics of DNA enzyme to catalyze reaction between chemiluminescent substrate H2O2-luminol to obtain a sensitive chemiluminescent signal, thus realizing high sensitive image immunoassay of a variety of protein. The immunosensor has the advantages of simple design, low cost, high sensitivity, high throughput and good repeatability, etc., and has certain clinical application value.

The invention discloses a photodynamic treatment medical composition which consists of a photosensitizer and a chemical activator, wherein the photosensitizer is expressed by the formula I; the chemical activator comprises luminol, p-iodophenol, horse radish peroxidase and hydrogen peroxide; and the proportion of the photosensitizer expressed by the formula I, to the luminol to the p-iodophenol to the horse radish peroxidase to the hydrogen peroxide in the composition is 8-12muM:0.4-0.7mM:1-1.5mM:0.01-0.02mg / mL:2-3mM. In the composition of the invention, active oxygen generated by bioluminescence energy transfer between the photosensitizer expressed by the formula I and the luminol is utilized to kill cells and fungi. Compared with the traditional photodynamic treatment method, the composition can be used for treating in deep tissues; and damages to normal tissues, which are caused by long-time irradiation by an external light source, can be avoided; and meanwhile, the composition has a profound influence on the aspects of the clinic treatment of tumors and pathogenic bacteria infection.

The invention discloses a preparation method and applications of a miRNA-21 electroluminescent immunosensor based on multi-functionalized molybdenum disulfide. The preparation method is characterized by comprising: carrying out amination on prepared Fe3O4 magnetic nanoparticles, and reacting with chloroauric acid to prepare Fe3O4@Au magnetic nanoparticles; carrying out carboxylation on prepared monolithic layer MoS2, sequentially reacting with a coupling reagent, a signal DNA solution and a luminol solution, finally adding a mercaptohexanol solution, and carrying out vibration washing to obtain a signal unit solution; carrying out a reaction on the Fe3O4@Au and a DNA capturing solution to obtain a capture unit solution; and coating the capture unit solution on the surface of a magnetic glassy carbon electrode in a dropwise manner, and then sequentially coating miRNA-21 and the signal unit solution on the surface of the magnetic glassy carbon electrode in a dropwise manner so as to obtain the product. The miRNA-21 electroluminescent immunosensor of the present invention has advantages of high sensitivity, strong specificity, high accuracy, simple operation step, and low experimental cost.

Provided is luminescent gold nanomaterial functionalized by N-(4-aminobutyl)-N-ethylisoluminol, methods of preparation and application thereof. The functionalized gold nanoparticle nanomaterial are formed by N-(4-aminobutyl)-N-ethylisoluminol bonding to the surface of the gold nanomaterial. The functionalized gold nanomaterial are prepared by directly reducing chloroauric acid with N-(4-aminobutyl)-N-ethylisoluminol, wherein N-(4-aminobutyl)-N-ethylisoluminol acts as reducer and stabilizer simultaneously. The preparation method is simple, fast and no need of special conditions. The preparation methods can be performed in a wide temperature range, for example, 15-35° C. The size and pattern of the functionalized gold nanomaterial can be specified by choosing the ratio of chloroauric acid to N-(4-aminobutyl)-N-ethylisoluminol. The obtained functionalized nano gold particles exhibit excellent chemiluminescence properties. Said functionalized gold nanomaterial can be combined with biomolecules to form biomolecular probe, which can be used for immunoassay, nucleic acid analysis, molecular imaging, sensor, etc., and has a broad application prospect in the field of clinical analysis biomedicine, food safety, and environment monitoring.

The invention discloses a biological sensor based on CuS nano-particle cation exchange enhanced chemiluminescence. Copper sulphide nano-particles are marked on biological molecules, and a composite structure marked with copper sulphide is obtained through a biological identification function; silver nitrate is added into a system, cation exchange reaction on silver nitrate and copper sulfide nano-particles is instantly carried out so as to generate copper ions; a target is quantitatively detected by utilizing chemiluminescence caused by catalyzing a luminol-hydrogen oxide system by the generated copper ions.