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Preparation method and applications of miRNA-21 electroluminescent immunosensor based on multi-functionalized molybdenum disulfide

A technology of molybdenum disulfide, luminescent immunity, applied in the field of functional materials and biosensing

Active Publication Date: 2017-04-19
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no relevant research reports on the preparation method and application of miRNA-21 electrochemiluminescence immunosensor based on multifunctional molybdenum disulfide at home and abroad.

Method used

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  • Preparation method and applications of miRNA-21 electroluminescent immunosensor based on multi-functionalized molybdenum disulfide
  • Preparation method and applications of miRNA-21 electroluminescent immunosensor based on multi-functionalized molybdenum disulfide
  • Preparation method and applications of miRNA-21 electroluminescent immunosensor based on multi-functionalized molybdenum disulfide

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Embodiment 1

[0041] A preparation method of miRNA-21 electrochemiluminescence immunosensor based on multifunctional molybdenum disulfide, such as figure 1 shown, including the following steps:

[0042] (1) Fe 3 o 4 Preparation method of @Au magnetic nanoparticles

[0043] a. Preparation of Fe 3 o 4 Magnetic nanoparticles: 0.85 g FeCl 3 ·6H 2 O and 0.35 g FeCl 2 4H 2 Dissolve O in 80 mL of secondary water and add it to a three-necked flask, pass nitrogen gas, stir and mix evenly with a magnetic force, raise the temperature to 90°C, slowly add 22wt% ammonia water to the three-necked flask, and adjust the pH to 9-10. After continuing to heat and stir for 1.5 h, stop heating, under the protection of nitrogen, stir and reflux, and cool to room temperature, the prepared black precipitate is Fe 3 o 4 For magnetic nanoparticles, wash with secondary water until neutral, and dilute to 50 mL in ethanol;

[0044] b. Preparation of aminated Fe 3 o 4 Magnetic nanoparticles: the Fe prepared ...

Embodiment 2

[0056] With above-mentioned embodiment 1, its difference is:

[0057] Step (1) Fe 3 o 4 In the preparation method of @Au magnetic nanoparticles: 0.7 g FeCl 3 ·6H 2 O and 0.2 g FeCl 2 4H 2 O was dissolved in 50 mL of secondary water and added to the three-necked flask, nitrogen gas was passed, magnetic stirring was used to mix evenly, the temperature was raised to 80°C, and 20wt% ammonia water was slowly added dropwise to the three-necked flask to adjust the pH=9~10, Continue heating and stirring for 1.5 h; Fe 3 o 4 The magnetic nanoparticle solution was sonicated for 0.5 h, 0.2 mL of 3-aminopropyltriethoxysilane was added, stirred for 4 h, 1 mL of 0.5 mol / L nitric acid solution was added, and stirred for 3 h; 5 mL of aminated Fe 3 o 4 Magnetic nanoparticle solution and 10 mL of 1wt% HAuCl 4 Mix at an ultrasonic frequency of 60 kHz, and after stirring for 1 h, slowly add 30 mL of 30 mmol / L trisodium citrate solution, and sonicate for 2 h.

[0058] In the synthesis met...

Embodiment 3

[0062] With above-mentioned embodiment 1, its difference is:

[0063] Step (1) Fe 3 o 4 In the preparation method of @Au magnetic nanoparticles: 1.0 g FeCl 3 ·6H 2 O and 0.5 g FeCl 2 4H 2Dissolve O in 100 mL of secondary water and add it to a three-necked flask, pass nitrogen gas, stir and mix evenly with a magnetic force, raise the temperature to 100°C, slowly add 25wt% ammonia water to the three-necked flask, and adjust the pH to 9-10. Continue heating and stirring for 2 h; Fe 3 o 4 The magnetic nanoparticle solution was sonicated for 1 h, 0.3 mL of 3-aminopropyltriethoxysilane was added, stirred for 5 h, 2 mL of 0.1 mol / L nitric acid solution was added, and stirred for 4 h; 10 mL of aminated Fe 3 o 4 Magnetic nanoparticle solution and 20 mL of 1wt%HAuCl 4 Mix at an ultrasonic frequency of 100 kHz, and after stirring for 1 h, slowly add 50 mL of 20 mmol / L trisodium citrate solution, and ultrasonicate for 3 h.

[0064] In the synthesis method of the signal unit in s...

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Abstract

The invention discloses a preparation method and applications of a miRNA-21 electroluminescent immunosensor based on multi-functionalized molybdenum disulfide. The preparation method is characterized by comprising: carrying out amination on prepared Fe3O4 magnetic nanoparticles, and reacting with chloroauric acid to prepare Fe3O4@Au magnetic nanoparticles; carrying out carboxylation on prepared monolithic layer MoS2, sequentially reacting with a coupling reagent, a signal DNA solution and a luminol solution, finally adding a mercaptohexanol solution, and carrying out vibration washing to obtain a signal unit solution; carrying out a reaction on the Fe3O4@Au and a DNA capturing solution to obtain a capture unit solution; and coating the capture unit solution on the surface of a magnetic glassy carbon electrode in a dropwise manner, and then sequentially coating miRNA-21 and the signal unit solution on the surface of the magnetic glassy carbon electrode in a dropwise manner so as to obtain the product. The miRNA-21 electroluminescent immunosensor of the present invention has advantages of high sensitivity, strong specificity, high accuracy, simple operation step, and low experimental cost.

Description

technical field [0001] The present invention relates to an electrochemiluminescence immunosensor and a detection method thereof, in particular to a preparation method and application of a miRNA-21 electrochemiluminescence immunosensor based on a multifunctional molybdenum disulfide material, belonging to functional materials and biological sensors. technology field. Background technique [0002] Cancer, also known as malignant tumor, is essentially a polygenic disease caused by the abnormal mechanism of cell growth and proliferation, which seriously endangers human health. According to WHO reports and predictions, cancer cases worldwide will show a rapid growth trend, from 14 million in 2012 to 19 million in 2025 and 24 million in 2035. Oncologists believe that early detection, early diagnosis, and early treatment of tumors can effectively prevent cancer, and early diagnosis is the key. The discovery of tumor markers makes early diagnosis possible. Tumor markers are a cla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/543G01N21/76
CPCG01N21/76G01N33/53G01N33/54326G01N33/54346
Inventor 卢静郭智勇胡宇芳沙玉红武琳俞欣辰郭富米
Owner NINGBO UNIV
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