41 results about "Heme-binding protein" patented technology

Methods and compounds for the treatment or prevention of oxidative damage in a mammalian subject. The treatment and / or prevention may be on inhibiting heme-induced lipid peroxidation. Also discloses are methods and compounds for treating or preventing isoprostane-mediated tissue damage.

An electrochemical biosensor enzyme function sensitive film containing nickel aluminum hydrotalcite nanometer plate and a preparation method belong to the technical field of electrochemical biosensor and its preparation. Wherein, the sensitive film comprises heme proteins, high molecule substances and nickel aluminum hydrotalcite nanometer plates. The preparation method of the sensitive film includes steps below: Hydrotalcite nanometer plate sols are mixed with heme proteins, dipped onto a clean glass carbon electrode surface then and submerged into the high molecule substance PVB solution for static placement after being dried at room temperature to volatilize the solvent, thus forming a layer of enzyme function sensitive film containing nickel aluminum hydrotalcite nanometer plate. The sensitive film of the present invention is suitable for determination of peroxide and has the advantages of simple production technique, lower cost and wide detecting scope, etc.

The invention provides a method for detecting protease activity and inhibitor activity thereof based on chemiluminescence, and relates to the technical field of biological analytical chemistry. The method for detecting the protease activity and the inhibitor activity thereof based on the chemiluminescence solves the problems that an existing method for detecting the enzyme activity is complex in flow design, complicated in operation, low in detection sensitivity, and high in cost. The method for detecting the protease activity and the inhibitor activity thereof based on the chemiluminescence comprises a first step of dissolving heme proteins into Tris - HCl buffer solution to achieve heme protein solution, a second step of adding protease with different concentrations into the heme protein solution of the first step to form a reaction system, and a third step of injecting the reaction system of the second step into a flow-injected chemiluminescence flow, and reacting a chemiluminescence reaction with a luminol hydrogen peroxide detecting system so as to detect the protease activity. The method for detecting the protease activity and the inhibitor activity thereof based on the chemiluminescence has the advantages of being simple, quick and high in sensitivity, low in cost, and low in detection limit, and the detection limit of trypsin can achieve to 1 ng / mL.

The invention relates to a heme protein functional magnetic surface enhanced Raman active substrate and an application thereof to the high-sensitivity detection of toxic micro-molecules and the rapid removal of the toxicity of the toxic micro-molecules, and belongs to the technical field of monitoring and detecting toxic ingredients in environment and food. The heme protein functional magnetic surface enhanced Raman active substrate is prepared by the following steps: synthesis, preparation and coating of a silver nano magnetic material, covalent adsorption of heme protein and spectrum detection of surface enhanced Raman scattering (SERS) and the like. The heme protein functional magnetic surface enhanced Raman active substrate and the application thereof have the beneficial effects that through the absorption of some toxic micro-molecules, the displacement of a heme protein resonance Raman peak can be caused, and the displacement has the molecular specificity, therefore, the micro-molecules can be subjected to multi-component detection by the utilization of an SERS spectrum; with the addition of the aggregation of magnetic nano materials under the magnetic field conditions, the concentration of molecules to be detected and the SERS enhancement capacity can be improved. The silver nano magnetic material in the heme protein functional magnetic surface enhanced Raman active substrate has a great application potential in the fields of food safety, environment monitoring and protection and the like.

The invention provides a premix mate for laying hens in an egg producing period and a preparation and application of the premix mate and belongs to the field of animal feeds. The premix mate for the laying hens in the egg producing period is prepared from the following raw materials in parts by weight: peanut meal, hot chili residues, fermented cake meal and amino acid, sodium bicarbonate, betaine, hemoprotein, limestone powder, mycoprotein, a shell treasure, calcium hydrophosphate, quercetin, a mildew inhibitor, perennial sowthistle powder, compound probiotics and the like. According to the premix mate for the laying hens in the egg producing period, one part of bean pulp in a complete formula feed can be replaced, the antioxidant activity of the laying hen bodies can be effectively improved, the digestion and absorption rate and the conversion rate of the laying hens in the egg producing period are effectively improved, the immunity is significantly improved, healthy intestinal tracts of the laying hens are ensured, too thin faeces are prevented, unhairing of the egg producing period is improved and slow growth condition of feather is improved. For the conditions of poor quality of egg shells, multiple broken eggs and a short peak laying period, the colors and the quality of the egg shells are effectively improved, the health of bodies is promoted, the production performance is improved and the egg production period is prolonged.

The functional characteristics of heme proteins can be modified to produce hemoglobins that can be used as blood substitutes in different therapeutic applications. Stable polymers of tetrameric hemoglobin, and of myoglobin molecules, are provided for use in the blood substitutes.

A cerebrospinal fluid (CSF) monitoring device for the detection of obstructions in real time through the collection of pressure data from an on-board pressure sensor. This system is designed to integrate into medical devices equipped with a CSF drainage system, such as implantable shunts. CSF analysis data can be transmitted in real-time wirelessly to a physician or can be stored on-board in a memory card and retrieved wirelessly at a later time. Information from the CSF sensors can be used by physicians to monitor CSF conditions known to cause shunt malfunction / obstruction such as elevated protein concentrations, evidence of an on-going or recent hemorrhage such as presence of heme proteins, and concentrations of administered medications / drugs over time. It can be used to monitor for CSF infections, in which increased pressure, increased temperature, CSF high protein and low glucose, would be suggestive of CSF infections.

The present invention provides an isolated archael and bacterial heme binding protein which reversibly binds oxygen with a low affinity. The heme binding protein may be utilized as a blood substitute. The invention also provides a method for controlled storage of oxygen by contacting a bacterial heme binding protein with oxygen allowing the protein to bind and store oxygen. The also provides methods to sense gaseous ligands using the heme binding protein. In other embodiments, the invention provides chimeric proteins having a heme-binding domain of an isolated heme binding archael bacterial protein and a heterologous signaling domain.

A method of stabilizing a hem protein which is effective against the denaturation and degradation of a hem protein typified by hemoglobin and a storage solution therefor. A method of stabilizing a hem protein and a storage solution therefor characterized in that an iminocarboxylic acid or its salt is made to coexist in a sample containing the hem protein, wherein the above-described iminocarboxylic acid is a compound represented by the following general formula (1) wherein R represents a hydrogen atom or a hydroxyl group; and X's represent each a hydrogen atom, an alkali metal or an ammonium group.

A process is disclosed for reducing the content of polycyclic aromatic hydrocarbons or PAHs in aromatic extracts including oxidizing the PAHs in the presence of a hemoprotein via an oxidizing compound, wherein the aromatic extract is brought into contact with the oxidizing agent in a non-reactive organic solvent, then is brought into contact with the immobilized or supported hemoprotein.

Provided herein are methods for producing products containing strained carbocycles, such as cyclopropene moieties and / or bicyclobutane moieties. The methods include combining an alkyne and a carbene precursor in the presence of a heme protein, e.g., a cytochrome P450, under conditions sufficient to form the strained carbocycle. Reaction mixtures for producing strained carbocycles are also described, as well as whole-cell catalysts comprising heme proteins and variants thereof for forming cyclopropenes, bicyclobutanes, and related products.

The present invention provides an electroconductive adhesive having a heme protein mixed in a very small amount thereby exhibiting electrical conductivity without deteriorating adhesion, which is used on the surface of electronic parts or a label thereby preventing and eliminating static electricity and improving the efficiency of heat dissipation, as well as an adhesive article using the same. A heme protein having a porphyrin complex whose iron ion is divalent is added to an adhesive to confer electrical conductivity. At least one member selected from an electroconductive polymer, an electrolytic metal, and a pigment molecule is mixed with the resultant electroconductive adhesive.

The invention discloses prescription paste for improving liver function of a pet. The paste is prepared from the following raw materials: schisandra chinensis extract, glycyrrhiza extract, angelica sinensis extract, astragalus extract, acanthopanax senticosus extract, codonopsis pilosula extract, radix paeoniae alba extract, inositol, heme protein powder, fish oil, bovine hydrolyzed paste, chickenpowder, sodium carboxymethylcellulose and zinc gluconate. The prescription paste for improving liver function of the pet plays roles of treating liver diseases of the pet in an auxiliary manner and improving the liver function of the pet. The invention also discloses a preparation method of the prescription paste for improving liver function of the pet. By heating and stirring most raw materialsuniformly and adding vitamins to heat, components centralized later are uneasy to be heated for a long time to be damaged, and the preparation method is simple and convenient to operate.

The invention relates to a fruit tree nutritional agent, which is composed of the following components by weight: 40% of hemoprotein powder, 30% of glucose powder, and 30% of agricultural borax powder. The preparation method consists of: weighing 2kg of animal blood and 10g of sodium chloride, placing them in a container and conducting heating at a temperature of 65-70DEG C for 20min, placing animal plasma into another container, and carrying out cooling for 20 minutes; putting the cooled animal plasma into centrifugal equipment and conducting centrifugation for 8 minutes, thus obtaining a hemoprotein raw material; placing the hemoprotein raw material in an outdoor site, and carrying out air drying treatment so as to obtain a blocky hemoprotein raw material; then putting the hemoprotein raw material into crushing equipment to implement crushing so as to obtain hemoprotein coarse powder, then placing the hemoprotein coarse powder into screening equipment with a sieve of 100 meshes to performing sieving treatment, thus obtaining the hemoprotein powder; and mixing the hemoprotein powder with the glucose powder and the agricultural borax powder in a weight ratio of 40%, 30% and 30% in order, then implementing packaging, thus obtaining the fruit tree nutritional agent.

The present invention provides compositions and methods for catalyzing the formation of carbon-silicon bonds using heme proteins. In certain aspects, the present invention provides heme proteins, including variants and fragments thereof, that are capable of carrying out in vitro and in vivo carbene insertion reactions for the formation of carbon-silicon bonds. In other aspects, the present invention provides methods for producing an organosilicon product, the method comprising providing a silicon-containing reagent, a carbene precursor, and a heme protein; and combining the components under conditions sufficient to produce an organosilicon product. Host cells expressing the heme proteins are also provided by the present invention.

The invention belongs to the technical field of biology, and discloses a neurored mutant protein and a preparation method thereof. The neurored mutant protein disclosed by the invention is based on genetic engineering and protein engineering technology, and neurored mutant protein A15C Ngb is obtained by introducing cysteine(Cys15) at the site 15 of human neuroglobin, with an amino acid sequence thereof as shown in SEQ ID NO. 1. The neurored mutant protein A15C Ngb prepared according to the invention has significantly improved pH stability, chemical denaturation stability and thermal stabilitycompared with WT Ngb, and can be widely used for studying the structure and function relationship of the human neuroglobin, and other heme protein molecular design based on the human neuroglobin.

The disclosure provides for compositions and methods for the preservation of meat tissues, including fish, beef, poultry and pork, and meat analogs containing added heme protein, using very low amounts of phospholipase A2 (PLA2) enzymes in a combination with rosemary.

An object of the present invention is to provide a novel preservative solution for a heme protein and a method for stabilizing a heme protein, which are effective against denaturation or degradation of a heme protein, and the present invention specifically relates to a preservative solution for a heme protein comprising a disulfonic acid or a salt thereof, and a method for stabilizing a heme protein, which involves bringing a disulfonic acid or a salt thereof into coexistence in a sample comprising a heme protein.

The invention provides mesenchymal stem cell protection liquid and application thereof. The protection liquid comprises, by weight, 0.2-1 part of AB-type umbilical cord blood plasma, 8-15 parts of hemoprotein, 4-8 parts of myohemoglobin, 5-10 parts of HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and 500-1500 parts of water solutions. The mesenchymal stem cell protection liquid has the advantages that rich nutrition is provided for mesenchymal stem cells, survival rate is maintained, and storage time is prolonged.

This invention relates, e.g., to a method for suppressing inflammation (e.g. allergic inflammation, for example asthma caused by ragweed), a Th1-mediated condition and / or a Th2-mediated condition and / or a condition in which administration of an antioxidant would be beneficial, in a subject in need thereof, comprising administering to the subject an anitinflammatory-effective amount of Ascaris-derived heme-binding protein (HBP) or an active fragment or variant thereof (e.g. domain 2 of the polypeptide or an active variant thereof). Also described are pharmaceutical compositions for suppressing one of the above conditions, adjuvant compositions and immunogenic compositions comprising Ascaris-derived heme-binding protein (HBP), or an active fragment or variant thereof, and an antigen.

The invention discloses a method for producing a heme-binding protein, which is characterized by comprising the following steps: transferring an expression vector containing a heme-binding protein gene expression cassette into a soybean genome and expressing to obtain soybean seeds rich in heme-binding protein, and separating and extracting to obtain the heme-binding protein. The invention provides a method for producing hemoglobin / myoglobin by using transgenic soybean seeds. The hemoglobin / myoglobin produced by the method can be used as a coloring additive and a flavor regulator of food; the invention discloses a promoter and a regulatory sequence which can be highly expressed in soybean seeds. The content of hemoglobin / myoglobin in the soybean seeds of a high-expression strain reaches 0.1% or above of the weight of the seeds; according to the hemoglobin / myoglobin produced from the transgenic soybeans, the production cost is reduced, energy is saved compared with a traditional microbial fermentation method, and the method is a more environment-friendly production mode.

A method of stabilizing a hem protein which is effective against the denaturation and degradation of a hem protein typified by hemoglobin and a storage solution therefor. A method of stabilizing a hem protein and a storage solution therefor characterized in that an iminocarboxylic acid or its salt is made to coexist in a sample containing the hem protein, wherein the above-described iminocarboxylic acid is a compound represented by the following general formula (1) wherein R represents a hydrogen atom or a hydroxyl group; and X's represent each a hydrogen atom, an alkali metal or an ammonium group.