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Biologic sensor enzyme functional susceptivity film containing nickel and aluminum hydrotalcite nano piece and method of producing the same

A nickel-aluminum hydrotalcite, biosensor technology, applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve the problems of blocking proteins and enzymes, difficult access to the electrode surface, and difficult exposure, etc., to achieve strong affinity, good electrochemical activity, Strong adsorption effect

Inactive Publication Date: 2008-03-12
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the electroactive centers of redox proteins and enzymes are usually buried deep inside their polypeptide chains, which are not easily exposed and difficult to access the electrode surface, thus hindering the direct electron transfer reaction of proteins and enzymes on the electrode surface.

Method used

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  • Biologic sensor enzyme functional susceptivity film containing nickel and aluminum hydrotalcite nano piece and method of producing the same
  • Biologic sensor enzyme functional susceptivity film containing nickel and aluminum hydrotalcite nano piece and method of producing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of A nickel-aluminum hydrotalcite nanosheets

[0035] Weigh 0.05mol of Ni(NO 3 ) 2 ·6H 2 O and 0.0125mol of Al(NO 3 ) 3 9H 2 O was dissolved in 100 mL deionized water. Weigh 0.2mol NaOH and dissolve in 100mL to remove CO 2 The concentration of water is 2mol L -1 alkaline solution. The saline solution was added dropwise while adjusting the dropping rate of the NaOH solution. Control the pH of the reaction system to be around 6.5. in N 2 Crystallization and reflux at 60° C. for 6 hours under protection. deCO 2 Wash with deionized water, filter, and vacuum-dry the filter cake at 20°C for 12 hours, weigh 0.05g of the resulting hydrotalcite product, dissolve it in 100ml of formamide, heat and stir at 80°C for 1.5 hours until 0.5g·L -1 Clarified nickel-aluminum hydrotalcite nanosheet sols. B Preparation of nickel-aluminum hydrotalcite nanosheets and horseradish peroxidase-sensitive membrane

[0036] Dissolve 1 mg of HRP into 100 μL of double-distill...

Embodiment 2

[0039] Preparation of A nickel-aluminum hydrotalcite nanosheets

[0040] Weigh 0.0375mol of Ni(NO 3 ) 2 ·6H 2 O and 0.0125mol of Al(NO 3 ) 3 9H 2 O was dissolved in 100 mL deionized water. Weigh 0.2mol NaOH and dissolve in 100mL to remove CO 2 The concentration of water is 2mol L -1 alkaline solution. The saline solution was added dropwise while adjusting the dropping rate of the NaOH solution. Control the pH of the reaction system to be around 6.5. in N 2 Crystallization and reflux at 70° C. for 8 hours under protection. deCO 2 Wash with deionized water, filter, and vacuum-dry the filter cake at 25°C for 14 hours, weigh 0.05g of the resulting hydrotalcite product, dissolve it in 50ml of formamide, heat and stir at 85°C for 2 hours until a clear 1g·L -1 NiAl hydrotalcite nanosheet sol.

[0041] B Preparation of nickel-aluminum hydrotalcite nanosheets and horseradish peroxidase-sensitive membrane

[0042] Dissolve 1 mg of HRP into 100 μL of double-distilled water...

Embodiment 3

[0045] Preparation of A nickel-aluminum hydrotalcite nanosheets

[0046] Weigh 0.025mol of Ni(NO 3 ) 2 ·6H 2 O and 0.0125mol of Al(NO 3 ) 3 9H 2 O was dissolved in 100 mL deionized water. Weigh 0.2mol NaOH and dissolve in 100mL to remove CO 2 The concentration of water is 2mol L -1 alkaline solution. The saline solution was added dropwise while adjusting the dropping rate of the NaOH solution. Control the pH of the reaction system to be about 6.5. in N 2 Crystallization and reflux at 80° C. for 12 hours under protection. deCO 2 Wash with deionized water, filter, and vacuum-dry the filter cake at 30°C for 20 hours, weigh 0.05g of the obtained hydrotalcite product, dissolve it in 33ml of formamide, heat and stir at 90°C for 3 hours until 1.5g L -1 NiAl hydrotalcite nanosheet sol. B Preparation of nickel-aluminum hydrotalcite nanosheets and horseradish peroxidase-sensitive membrane

[0047] Dissolve 1 mg of HRP into 200 μL of double-distilled water, adjust the rati...

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Abstract

An electrochemical biosensor enzyme function sensitive film containing nickel aluminum hydrotalcite nanometer plate and a preparation method belong to the technical field of electrochemical biosensor and its preparation. Wherein, the sensitive film comprises heme proteins, high molecule substances and nickel aluminum hydrotalcite nanometer plates. The preparation method of the sensitive film includes steps below: Hydrotalcite nanometer plate sols are mixed with heme proteins, dipped onto a clean glass carbon electrode surface then and submerged into the high molecule substance PVB solution for static placement after being dried at room temperature to volatilize the solvent, thus forming a layer of enzyme function sensitive film containing nickel aluminum hydrotalcite nanometer plate. The sensitive film of the present invention is suitable for determination of peroxide and has the advantages of simple production technique, lower cost and wide detecting scope, etc.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensors and preparation thereof, in particular to an electrochemical biosensor enzyme function sensitive membrane containing nickel-aluminum hydrotalcite nanosheets and a preparation method thereof. Electrodes modified with this sensitive membrane can be used for H 2 o 2 detection. Background technique [0002] Electrochemical biosensors have the characteristics of stable performance, high selectivity, easy operation, and easy miniaturization, and have been widely used in clinical diagnosis, industrial control, food testing, drug analysis, environmental analysis, biochips, and military fields. Among them, the third-generation reagent-free electrochemical biosensor, which uses the direct electrochemical behavior (ie, direct electron transfer) between the bioactive substance and the electrode for detection, has attracted more attention and is a hot spot in the field of electrochemical b...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 陈旭付呈琳杨文胜王毅
Owner BEIJING UNIV OF CHEM TECH
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