The invention relates to an
environmental analysis detection method, and specifically, relates to a
high performance liquid chromatography determination method of
tetracycline antibiotic in soil. The
high performance liquid chromatography determination method of
tetracycline antibiotic in soil comprises the following steps of 1, a sample collecting and preparation process comprising collecting soil in a
plough layer having thickness of 0 to 20 centimeters, wherein soil in a composting place and field edges is not adopted, uniformly mixing multiple single-point samples, carrying out quarteringreduction to obtain a mixed soil sample having weight of about 1kg, putting the mixed soil sample into a sealing bag, bringing the sealing bag with the mixed soil sample back with low-temperature preservation in the dark, putting the mixed soil sample into a room,
drying in air, crushing, screening by a nylon
sieve of 60 meshes, and weighing 2g of the mixed soil sample for next use, 2, an extractpreparation process, 3, an extraction, purification and enrichment process, and 4, an
elution, volume metering and detection process. The
high performance liquid chromatography determination method of
tetracycline antibiotic in soil has normalized processes, reduces a
vacuum drying process, produces low jamming, shortens
operation time and improves operation accuracy. Through connection between astrong anion
solid phase extraction column and a C18 inverted
solid phase extraction column in series, purification and enrichment effects are greatly improved.