3519 results about "FluoProbes" patented technology

The spectroscopic and photophysical properties of fluorescent probes comprising donor-acceptor derivatives comprising the boric acid group or a derivative of boric acid, B(OH)3 (or borate ion, BO(OH)2<-1>), arsenious acid, H3 AsO3 (or arsenite ion, H2AsO3<-1>), telluric acid, H6TeO6 (or tellurate ion, H5 TeO6<-1>) or germanic acid, Ge(OH)6 (or germanate ion, GeO(OH)3<-1>) are described. Method of using said probes are also provided.

The invention belongs to the technical field of nanometer biological materials, and particularly relates to an 800nm excitation-based up/down conversion dual-mode fluorescent nanomaterial for Nd<3+> sensitization and a synthesis method thereof. The synthetic up/down conversion dual-mode fluorescent nanomaterial for Nd<3+>sensitization designed by the invention has a multi-layer core-shell structure, and comprises a down conversion luminous layer, an up conversion luminous layer, an isolation layer and a passivation layer. Different layers of the material synergistically play respective different roles, and meanwhile, the dual-mode fluorescent probe with up conversion fluorescence and down conversion fluorescence is finally achieved under an 800nm of excitation light with a low-heat effect. The up conversion excitation light is expanded to about 800nm from 980nm by Nd<3+>, Yb<3+> and Er<3+>-doped NaGdF4:Nd, Yb, Er up conversion layer due to introduction of Nd<3+>, and the fluorescent process from a near infrared light to a visible light is achieved. The process meets the requirements of an in-vitro fluorescent probe. In addition, the typical down conversion fluorescence from the near infrared light to a far infrared light is also achieved in one nanoparticle due to the synergistic effect of the NaGdF4:Nd core.

The invention discloses a nitrogen-doped fluorescent carbon quantum dot and a preparation method and application thereof, and belongs to the technical field of material science. Fish wastes (such as fish scales, bones and skins) are cleaned, then hydrothermal reaction is directly performed to obtain nitrogen-doped fluorescent carbon quantum dot aqueous dispersion, and finally filtration, dialysis and freeze-drying are performed to obtain nitrogen-doped fluorescent carbon quantum dot solid. The size of the nitrogen-doped fluorescent carbon quantum dot prepared by using the preparation method disclosed by the invention is smaller than or equal to 10nm, the nitrogen content is smaller than or equal to 20 percent, the surface of the quantum dot has nitrogen-containing and oxygen-containing functional groups, and the quantum efficiency is 17-30 percent. The nitrogen-doped fluorescent carbon quantum dot and the preparation method and application thereof have the advantages that the preparation method is environmental-friendly, the operation is simple, the requirements on equipment are low, the preparation method is suitable for large-scale production, and the prepared nitrogen-doped fluorescent carbon quantum dot has excellent fluorescent performance and can be widely applied to fields such as photoelectric materials, biological imaging and fluorescent probes.

The invention belongs to the field of application of near-infrared laser, nuclear irradiation, electron and image rebuild in systematic biology and medicine. It is characterized in that high-specificity nuclear species and fluorescent probe are implanted in live animal to realize double mark of muscle deep layer or internal organ tumor cell. The emission signal of nuclear species is checked by sensitive electron-multiplier phototube in array copulation position of scintillation crystal; feeble fluorescent signal generated by visible light or infrared agitation is checked by high quantum yield CCD camera. The two checking systems are orthogonally arranged in the same surface, the live nuclear species and fluorescent signal can be checked simultaneously through rotary image cavity, and double module three dimensional tomography image of small animal can be rebuilt with software algorism. The invention is characterized by multi-image forming modules, abundant information and simple operation.

The invention discloses a visible and reversible fluorescent probe which comprises a cyanine fluorescent group and a benzothiazole group, and the general formula of the probe is shown in the description. The preparation method of the reversible fluorescent probe comprises the following steps: (1) dropwise adding phosphorus oxychloride into salicylaldehyde and paraformaldehyde for reaction, so as to obtain a product 1; (2) enabling the product 1 and hexamethylene-tetramine to react, so as to obtain a product 2; (3) enabling the product 2 and 2-aminobenzenethiol to react at the room temperature, so as to obtain a product 3; (4) enabling the product 3 and a compound 4 to react, so as to obtain the visible and reversible fluorescent probe. According to the ratiometric fluorescent probe provided by the invention, obvious color variation can be found out under natural light or an ultra-violet lamp, and the qualitative detection of sulfur dioxide gas can be implemented under natural light or a hand-held ultra-violet lamp, so that the operation is simple, high convenience and quickness are achieved, and the effect is remarkable; the fluorescent probe can effectively prevent interference from other impurities in samples, and is excellent in selectivity; in addition, the cumbersome pre-processing process of samples is avoided, so that the detection efficiency is high.

The invention provides a phenanthrene and imidazole-coumarin double-fluorescent group ratio fluorescent molecular probe for iron ion detection and synthesis and use methods thereof, relates to fluorescent molecular probes and synthesis and application thereof and aims to solve the problem that an existing Fe<3+> fluorescent probe is prone to being interfered by pH, concentration and other metal ions. The fluorescent molecular probe is 4-methyl-7-hydroxide radical-8-[2-(1- phenyl group-1H-phenanthrene and [9, 10-d] imidazole-2-)benzene ammonia methylene]-2H-pyran-2-ketone. The phenanthrene and imidazole-coumarin double-fluorescent group ratio fluorescent molecular probe is formed by conducting condensation on 1-N-phenyl group-2-(2-aminophenyl)-1H-phenanthrene and [9, 10-d] imidazole and 4-methyl-7-hydroxide radical-8- formyl group coumarin, and the yield is 75-85%. The fluorescent molecular probe is dissolved in mixed liquid of N, N- dimethylformamide and an HEPES buffering solution, existence of iron ions is judged through the absorbance value or fluorescence intensity change before and after adding of test samples, and the fluorescent molecular probe can be used for detection of Fe<3+> pollution in water.

The invention discloses a multi-shell-structure quantum dot composite particle, and a high-fluorescent-brightness quantum dot probe and a preparation method thereof. The composite particle uses a quantum dot as a core, a semiconductor shell is coated outside the quantum dot, and a composite silicon dioxide shell is coated outside the semiconductor shell. A biological molecule can be connected to the composite silicon dioxide shell to form the probe. The composite particle and probe with required fluorescent wavelength can be obtained by controlling the reaction conditions. The method has strong operability. The obtained composite particle has favorable chemical and physical stability, biocompatibility and environmental stability. The formed probe has high stability and high fluorescent brightness, can have high application value in the fields of medicine and biology, and can be used as a common fluorescent probe, immunologic detection agent or any other biosensor.

The invention relates to a preparation method and application of a formaldehyde fluorescent probe, and belongs to the technical field of analytical chemistry. The formaldehyde fluorescent probe is a ratio-type fluorescent probe with double emission bands. The excitation wavelength is 318 nm; and the fluorescence peak at 359nm decreases gradually with the increasing of the concentration of formaldehyde, and at the same time a new emission band is produced at 451 nm and the fluorescence peak gradually increases. The formaldehyde fluorescent probe can resist the interference of acetaldehyde, glyoxal, methylglyoxal, benzaldehyde, alanine, glycine, serine, arginine, cysteine, glutathione and glucose, has high detection accuracy, high sensitivity and strong anti-interference capability. In addition, the formaldehyde fluorescent probe of the invention can detect the formaldehyde in a biological sample (cell environment), realizes formaldehyde fluorescence imaging in living cell level, and has potential practical application value. The synthesis of the formaldehyde fluorescent probe only needs a one-step reaction, which is simple and high in yield.

The invention provides an N, P and S-codoped fluorescent carbon quantum dot and a preparation method and application thereof, and belongs to the technical field of luminescent nanomaterials. The preparation method comprises the steps that a biological bacterium (saccharomycete or escherichia coli or staphylococcus aureus or aspergillus niger) is taken as a carbon source, water of the corresponding volume is added, and a hydrothermal reaction is performed to obtain a dark brown solution; after the reaction stops and a reaction kettle is naturally cooled, dialysis is performed to remove impurities, an aqueous carbon quantum dot solution is obtained, and after freeze drying is performed, the N, P and S-codoped fluorescent carbon quantum dot is obtained. According to the method, the biomass is taken as the carbon source, the raw material is wide in source, low in cost, green and environmentally friendly, the technology is simple, and the preparation condition requirement is low. The obtained carbon quantum dot can be applied to detection on Cr (VI) ions, MnO4<-> and ascorbic acid by serving as a switch-type fluorescent probe and also can be used for cell imaging.

The invention relates to preparation and application methods of ratio-dependent bisulfite ion fluorescent probes, belonging to the technical field of anion detection. The probe molecules have recognition specificity for bisulfite ions in a neutral aquatic environment, generate fluorescence ratio variations, have very high sensitivity, and meanwhile, cause obvious color and fluorescent color variations. The fluorescent probes do not generate obvious variations for other ions, free radicals or molecules. The invention implements recognition specificity of the probe molecules for the bisulfite ions, provides the fluorescent probes with color comparison sensing function, and thus, has wide application prospects in the fields of biology and ion detection.

The invention provides a fluorescent probe represented by the formula (I), wherein R1 and R2 are independently selected from H or an aromatic vinyl group, and at least one of R1 and R2 is the aromatic vinyl group; R3 is selected from H, F, Cl, Br, OH, OCH3, N(CH3)2 or C1-C6 alkyl; and R4 is selected from C1-C6 alkyl. Compared with the prior art, because the fluorescent probe provided by the invention has a relatively large electronic conjugated system and plane, the intensity of the intramolecular charge transfer effect can affect the molecular fluorescence emission intensity; when the fluorescent probe and the G-quadruplex generate a specific action, the flexibility of intramolecular rotatable double bonds is restricted, the intramolecular charge transfer effect is enhanced, and the fluorescence is also enhanced significantly; moreover, the fluorescent probe provided by the invention has the advantages of low biotoxicity, phototoxicity and photobleaching property, good light stability, good water solubility and good cell membrane permeability.

The invention belongs to the technical field of nano biological materials and particularly relates to a single-emission up-conversion nano fluorescent probe and a synthetic method thereof. The fluorescent probe is a nanocrystal with a structure comprising a core layer and three shell layers and comprises an up-conversion luminescence center core layer, an inert shell layer, a silicon dioxide shell layer and a dye-doped silicon dioxide shell layer; an active ion doped rare earth nanocrystal is arranged at the up-conversion luminescence center, and the inert shell layer completely covers the luminescence core; the silicon dioxide shell layer can avoid fluorescence resonance energy transfer between the up-conversion luminescence center and dye; the dye-doped silicon dioxide shell layer is used for removing unwanted up-conversion emission peaks and reserving specific up-conversion emission peaks, and single-emission up-conversion fluorescence is realized. By means of the probe and the method, multiple signal modules can be detected in situ simultaneously, and further, the detection sensitivity and the accuracy are improved. The fluorescent probe has a broad application prospect in the aspects of protein expression, high throughput screening of biological samples, multi-channel biological detection, disease diagnosis and the like.

The invention relates to a specific fluorescent probe for identifying hydrogen sulfide and an application of the probe, belonging to the field of fine chemical engineering. The fluorescent probe is a resorufin derivative. Resorufin sodium salt, potassium carbonate and 2, 4-binitro bromobenzene are mixed in proportion in acetonitrile liquor to be heated, and finally are purified by silica gel chromatography to obtain the fluorescent probe. The fluorescent probe and a corresponding hydrogen sulfide content detection process are not interfered by matrixes and impurities in a biosystem and can be used for quantitative determination of hydrogen sulfide content in various biosystems. The fluorescent probe has high specificity and can be hydrolyzed with hydrogen sulfide after being specifically cyclized to obtain a hydrolysate with broken ether bonds. The probe is low in cost and feasible, can be obtained by chemical synthesis and is simple and feasible in synthetic process. The probe is high in sensitivity, and has good fluorescence emission spectrum characteristics (600-650nm). In the wavelength range, the background fluorescence of a biological sample is weak, so that the probe is suitable for detecting hydrogen sulfide content in cells. Hydrogen sulfide is quantitively detected by drawing a standard curve.

The invention relates to a heptamethine cyanine active fluorescent probe and a preparation method and application thereof. The structural formula of the heptamethine cyanine active fluorescent probe is as shown in the specification, wherein X=II-IX; each of R1 and R2 is (CH2)mCH3, (CH2)nOH, (CH2CH2O)pCH3 and CH2C6H5; each of R3 and R4 is H, SO3H, SO3Na and SO3K; each of a, b, c, d, e, f and g is 2-8; each of n, m and p is 1-10. The heptamethine cyanine active fluorescent probe has the advantages that the heptamethine cyanine active fluorescent probe is based on near-infrared long-wave heptamethine cyanine dye, indoline is selected as the aroma parent nucleus to increase fluorescence intensity, and methenyl chain intermediate cyclohexene rigid bridging enhances stability; nitrogen derivatives with chemical reactivity sites are used to perform nucleophilic substitution on the meso-position of the heptamethine cyanine parent dye, and accordingly Stokes shift and active chemical groups areincreased greatly to facilitate the fluorescent labeling of various substances; the fluorescent probe is of a symmetrical structure, preparation and purification processes are simplified, and cost islowered favorably; the probe can be used as the fluorescent labeling probe of biological molecules such as high-sensitivity protein, sugar and DNA and nano carriers to perform cell or living-body horizontal fluorescence imaging, and the like.

The invention provides a method for preparation of water-soluble luminous graphite-phase carbon nitride nano kelp. The water-soluble luminous graphite-phase carbon nitride nano kelp is obtained by high-temperature polycondensation of precursors containing carbon and nitrogen in a mixed molten chloride salt system. The method is universal, and target products can be obtained by adoption of various precursors; the prepared nano kelp is uniform in shape, excellent in dispersibility in water, capable of forming high-concentration stable transparent colloidal solution and stable in existence in both alkaline and weak acidic environments; under excitation of ultraviolet light, solid nano kelp and the colloidal solution thereof both have strong and stable blue photoluminescence. On the basis of excellent photoluminescence performance and water solubility, the nano kelp is expected to serve as fluorescent probes to be applied to the fields of bioimaging, biomedical engineering, analysis, monitoring and the like, and large-scale production is expected to realize. Furthermore, the method for preparation of the water-soluble luminous graphite-phase carbon nitride nano kelp has the advantages of simplicity in operation, environment friendliness, low cost and the like.

The invention relates to a BODIPY-based high-sensitivity fluorescent probe and a synthesis method and application thereof. A structural general formula of the probe is shown as (I), wherein Trigger is stimulant triggering groups, R1 and R2 are groups for regulating and controlling fluorescent transmission wavelength of the probe and introducing organelle targeting, and R1 and R2 are defined in the description. By the probe, detection of different substrates can be realized without changing mother nucleus structure of the probe by only changing different Trigger groups. In addition, according to different needs on wavelength and targeting, the mother nucleus structure of the probe can be quickly modified. By changing R1 and R2, maximum fluorescent emission wavelength of the probe can be changed, and the probe can target mitochondrion to realize detection of active substances in the mitochondrion. The probe has good biocompatibility, thereby being applicable to detecting biological systems. Application value of the fluorescent probe in the aspect of detecting bioactive molecules and protease over-expressed in inflammatory or tumor tissue has potential social benefit and economic benefit.

The invention discloses a method for determining the CMC (critical micelle concentration) of surfactant. The new method for determining the CMC of surfactant is designed by using an aggregation-induced emission organic substance having a structure shown in Formula (I) as a fluorescent probe according to the characteristics of the organic substance. The method is to determine the CMC through the highest fluorescence intensity mutation point of the probe, while the CMC fluorescent determination method reported by others is to determine the CMC through the lowest fluorescence intensity mutation point of the probe. Therefore, the CMC fluorescent determination method disclosed by the invention has higher sensitivity in comparison with the reported CMC fluorescent determination method. According to the CMC fluorescent determination method disclosed by the invention, when surfactant detection solutions of different concentrations are prepared, the concentration of the fluorescent probe does not need to be maintained at the same level, thus greatly simplifying the operation steps and eliminating the error caused by measuring the fluorescent probe repeatedly. The Formula (I) is shown in the specification.

The invention provides a fluorescence probe, the synthetic method and the use which is to test the cell hydroxyl free radical. The process is: a. heat and chloroauric acid of 150.0 weight concentration and back flow, with mixing, the sodium citrate of 5.0-7.0 is added into the solution to back flow for 10-20min, then to cool in room temperature to get the golden nanometer particle solution; b. mix the particle with the DNA single chain with 5'end modified fluorescein and 3'end modified sulfydryl according to 1:200-500, then to set in room temperature for 1224h, last to adjust the pH by the phosphoric buffer; next to mix the mixture with the 0.5-5mol/L NaCl according to 1:0.1-0.3(V/V) which is set in room temperature for 35-45h; last to centrifugate to remove the upper solution and the deposition is solved in the 0.1-0.3mol/L phosphoric buffer which is the fluorescence probe solution.

The invention discloses a preparation method of a quantum dot@Cu nano-cluster ratiometric fluorescent sensor and application thereof in Cu<2+> detection, belonging to the technical field of fluorescence sensing. According to the preparation method, CuNCs prepared by taking polyethyleneiminen as a template is coupled to the surface of silicon dioxide coated CdSe QDs to prepare CdSe QDs@CuNCs ratiometric fluorescent sensor having CdSe QDs and CuNCs double-fluorescence emission signals. When Cu<2+> exists in a solution, amino of polyethyleneimine and Cu<2+> are subjected to complexing to form a copper-amine complex, thereby avoiding the problem that CuNCs fluorescence quenching is caused but the fluorescence intensity of CdSe QDs is unchanged because electrons are transferred from amino to CuNCs. With the increase of Cu<2+> concentration, the intensity ratio of two fluorescence emission peaks of CdSe QDs and CuNCs is reduced gradually, thereby realizing sensitive fluorescent and visual detection on Cu<2+>.

The invention discloses fluorescent wide chroma test paper for visually detecting copper ions as well as a preparation method and application of the test paper. The preparation method comprises the following steps: by taking a three-color ratio fluorescence probe as ink and taking filter paper as a solid-phase carrier through an inkjet printer, uniformly printing, thereby obtaining the test paper capable of visually detecting the copper ions, wherein the three-color ratio fluorescence probe refers to blue carbon dots, green quantum dots and red quantum dots, and the quantum dots are all modified by 3-mercaptopropionic acid. The three-color ratio fluorescence test paper is constructed to be used for visually detecting the copper ions by utilizing double-quenching principle first, the prepared detection test paper has the advantage that the color change range is wide compared with the conventional double-ratio fluorescence test paper, and along with addition of the detection object, test paper colors can be obviously identified by naked eyes from light scarlet, light flesh color, deep orange, grass green, dark olive green, slate blue, royalblue to cyanic color (Figure 4).

The invention relates to a kit and a method for detecting polymorphism of CYP2C19 gene, and belongs to the field of fluorogenic quantitive PCR (Polymerase Chain Reaction). The kit comprises a detection primer and a fluorescent probe, which comprise at least one group of specific primer and specific Taqman fluorescent probe of CYP2C19 gene CYP2C19*2 polymorphism, specific primer and specific Taqman fluorescent probe of CYP2C19 gene CYP2C19*3 polymorphism, and specific primer and specific Taqman fluorescent probe of CYP2C19 gene CYP2C19*17 polymorphism. The kit is adopted for detecting the CYP2C19 gene, the sensitivity and specificity are both obviously improved, the detection time is short and the predication of medicament dosage is facilitated.

The invention discloses a method for recognizing quantitative chiral amino acid by using a reversible nano porphyrin fluorescence sensor, and belongs to the technical field of nano material preparation and chemical analysis detection. The reversible nano porphyrin fluorescence sensor for specifically recognizing quantitative proline, lysine and serine chirality adopts a CdTe quantum dot as a fluorescence probe, and self-assembled porphyrin prepared from a tetra-(4-pyridyl) zinc porphyrin tetrahydrofuran solution and cetyl trimethyl ammonium bromide (CTAB) as a fluorescence quencher, and with the specific combination of the florescence probe and the fluorescence quencher, an openable/closeable nano porphyrin fluorescence sensor can be prepared. The reversible (opening-closing-opening) nano porphyrin fluorescence sensor can be prepared under the action of the openable/closeable nano porphyrin fluorescence sensor with the chiral proline, lysine and serine. Compared with a conventional chromatographic method for recognizing amino acid chirality by separating, the method disclosed by the invention has multiple advantages.