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Single-emission up-conversion nano fluorescent probe and synthetic method thereof

A technology of nano-fluorescent probes and synthesis methods, applied in the field of single-emission up-conversion nano-fluorescent probes and their synthesis, to achieve the effect of improving sensitivity and accuracy and broad application prospects

Active Publication Date: 2015-07-29
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the multi-level characteristics of rare earth ions doped in upconversion materials, materials obtained by doping with different rare earth ions or doping with different concentrations have multiple emission peaks, and different materials have overlapping spectra, which limits its application. , if the up-converting nanoparticles with single-wavelength emission can be obtained through some structural design, it will be an ideal multiple detection fluorescent probe, which has wider applications in biological analysis and disease detection

Method used

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  • Single-emission up-conversion nano fluorescent probe and synthetic method thereof
  • Single-emission up-conversion nano fluorescent probe and synthetic method thereof
  • Single-emission up-conversion nano fluorescent probe and synthetic method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] NaGdF 4 : 20 % Yb / 0.2 % TmNaGdF 4 SiO 2 SiO 2 - Preparation of NPTAT single blue light emitting upconversion nanocrystals. Specific steps are as follows:

[0026] (1) Preparation of the shell precursor. Preparation of Gd-OA (0.1 M) precursor: Take a 50 mL three-neck round bottom flask as the reaction vessel, add 2.5 mmol GdCl 3 , 10 mL oleic acid (OA), 15 mL octadecene (ODE). Heating to 140 °C for 1 h under vacuum and magnetic stirring conditions finally yielded a clear and transparent Gd-OA (0.1 M) precursor.

[0027] Preparation of Na-TFA-OA precursor: Take a 25 mL three-necked round-bottomed flask as a reaction vessel, add 4 mmol sodium trifluoroacetate and 10 mL oleic acid in sequence, dissolve at room temperature under vacuum and stirring conditions, and obtain a colorless, transparent and clear Na-TFA-OA precursor solution.

[0028] (2) NaGdF 4 : Synthesis of 20 % Yb / 0.2 % Tm upconverting nanocrystalline nuclei

[0029] Take a 100 mL three-neck round bot...

Embodiment 2

[0037] NaGdF 4 : 20 % Yb / 2 % ErNaGdF 4 SiO 2 SiO 2 - Preparation of NPTAT single green light-emitting upconversion nanocrystals. Specific steps are as follows:

[0038] (1) Preparation of the shell precursor. Preparation of Gd-OA (0.1 M) precursor: Take a 50 mL three-neck round bottom flask as the reaction vessel, add 2.5 mmol GdCl 3 , 10 mL oleic acid (OA), 15 mL octadecene (ODE). Heating to 140 °C for 1 h under vacuum and magnetic stirring conditions finally yielded a clear and transparent Gd-OA (0.1 M) precursor.

[0039] Preparation of Na-TFA-OA precursor: Take a 25 mL three-necked round-bottomed flask as a reaction vessel, add 4 mmol sodium trifluoroacetate and 10 mL oleic acid in sequence, dissolve at room temperature under vacuum and stirring conditions, and obtain a colorless, transparent and clear Na-TFA-OA precursor solution.

[0040] (2) NaGdF 4 : Synthesis of 20 % Yb / 2 % Er upconverting nanocrystalline nuclei

[0041] Take a 100 mL three-neck round bottom...

Embodiment 3

[0049] NaYbF 4 : 10 % ErNaYF 4 SiO 2 SiO 2 - Preparation of RhB isothiocyanate single red-emitting upconversion nanocrystals. Specific steps are as follows:

[0050] (1) Preparation of the shell precursor. Preparation of Y-OA (0.1 M) precursor: Take a 50 mL three-neck round bottom flask as the reaction vessel, add 2.5 mmol YCl in sequence 3 , 10 mL oleic acid (OA), 15 mL octadecene (ODE). Heating to 140 °C for 1 h under vacuum and magnetic stirring conditions finally yielded a clear and transparent Y-OA (0.1 M) precursor.

[0051] Preparation of Na-TFA-OA precursor: Take a 25 mL three-necked round-bottomed flask as a reaction vessel, add 4 mmol sodium trifluoroacetate and 10 mL oleic acid in sequence, dissolve at room temperature under vacuum and stirring conditions, and obtain a colorless, transparent and clear Na-TFA-OA precursor solution.

[0052] (2) NaYbF 4 : Synthesis of 10 % Er upconverting nanocrystalline nuclei

[0053] Take a 50 mL three-necked round-bottom f...

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Abstract

The invention belongs to the technical field of nano biological materials and particularly relates to a single-emission up-conversion nano fluorescent probe and a synthetic method thereof. The fluorescent probe is a nanocrystal with a structure comprising a core layer and three shell layers and comprises an up-conversion luminescence center core layer, an inert shell layer, a silicon dioxide shell layer and a dye-doped silicon dioxide shell layer; an active ion doped rare earth nanocrystal is arranged at the up-conversion luminescence center, and the inert shell layer completely covers the luminescence core; the silicon dioxide shell layer can avoid fluorescence resonance energy transfer between the up-conversion luminescence center and dye; the dye-doped silicon dioxide shell layer is used for removing unwanted up-conversion emission peaks and reserving specific up-conversion emission peaks, and single-emission up-conversion fluorescence is realized. By means of the probe and the method, multiple signal modules can be detected in situ simultaneously, and further, the detection sensitivity and the accuracy are improved. The fluorescent probe has a broad application prospect in the aspects of protein expression, high throughput screening of biological samples, multi-channel biological detection, disease diagnosis and the like.

Description

technical field [0001] The invention belongs to the technical field of nano-biological materials, and in particular relates to a single emission up-conversion nano-fluorescence probe and a synthesis method thereof. Background technique [0002] In recent years, cancer has become a global public health problem. Early detection and treatment can effectively reduce cancer mortality, so early diagnosis of cancer is of great significance. Most malignant tumors (especially breast and prostate cancers) are highly heterogeneous, containing a mixture of benign cells, malignant cells, fibroblasts, and other stromal cells, vascular cells, and infiltrating inflammatory cells (macrophages and lymphocytes). wait). The diagnosis and prognostic classification of human tumors are currently mainly based on immunohistochemical methods, which can only detect one substance to be tested at a time, and immunohistochemistry is semi-quantitative and the detection results are relatively subjective. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/85C09K11/02B82Y30/00B82Y40/00B82Y20/00G01N21/64
Inventor 张凡周磊
Owner FUDAN UNIV
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