The invention discloses a
fermentation method for the production of recombination
protein by
lactose-induced pMFH carrier. According to the invention, expression of
target protein is induced by successfully replacing IPTG with
lactose as
inducer mainly on the basis of formula of optimized
fermentation culture medium, formula of feeding liquid prior to inducement, formula of feeding liquid subsequent to inducement and formula of
lactose inducing liquid. The expression of the
target protein is high, and level of the
target protein in
total protein of strain can reach 35 to 49%, which is equivalent to that IPTG inducing level. In addition, after lactose is added for inducement in the process of
fermentation, glycerin is also added to serve as
carbon source, which can not only raise
biomass remarkably, but also reduce the generation of byproducts of
metabolism, i.e.
acetic acid, and avoid the influence of the presence of glucose in the fermentation liquid on lactose inducing effect. Obviously, the method according to the invention is not only simple and convenient, short in fermentation time and low in production cost, but also achieves no residual toxic substances in the
resultant product.