The invention discloses a natural yeast powder, which is prepared by the method comprising the following steps of: preparing a yeast solution soaking fruit, grains or plant flowers into honey, malt extract and purified water, and extracting the yeast solution of the fruit, grains or plant flowers containing saccharomyces cerevisiae strains and lactobacillus strains after placing; preparing yeast paste: kneading rye flour, the malt extract and the purified water into the yeast paste to be cultivated for four stages; cultivation of microzyme: combining the yeast solution with the yeast paste into wet cenobium for cultivation; enlarging cultivation of the microzyme: mixing the wet cenobium with an enlarging cultivation medium according to a certain ratio to carry out enlarging cultivation of the microzyme multiplying 100-10000 times, and preparing a mixed yeast solution; and concocting yeast powder: mixing dry substances such as glucose, alpha dry rice meal, vitamin C, corn starch, and cane sugar into the mixed yeast solution to finally obtain the natural yeast powder. The natural yeast powder is completely prepared by natural products, does not contain a preservative, and has the advantages of ageing resistance, anti-mildew and long retention period.