80 results about "Lysophosphatidic acid" patented technology

Autotaxin (ATX) is a prometastatic enzyme initially isolated from the conditioned media of human melanoma cells that stimulates a myriad of biological activities including angiogenesis and the promotion of cell growth, survival, and differentiation through the production of lysophosphatidic acid (LPA). ATX increases the aggressiveness and invasiveness of transformed cells, and ATX levels directly correlate with tumor stage and grade in several human malignancies. To study the role of ATX in the pathogenesis of malignant melanoma, we developed antibodies and small molecule inhibitors against recombinant human protein. Immunohistochemistry of paraffin embedded human tissue demonstrates that ATX levels are markedly increased in human primary and metastatic melanoma relative to benign nevi. Chemical screens identified several small molecule inhibitors with binding constants ranging from nanomolar to low micromolar. Cell migration and invasion assays with melanoma cell lines demonstrate that ATX markedly stimulates melanoma cell migration and invasion, an effect suppressed by ATX inhibitors. The migratory phenotype can be rescued by the addition of ATX's enzymatic product, LPA, confirming that the observed inhibition is linked to suppression of LPA production by ATX. Chemical analogues of the inhibitors demonstrate structure activity relationships important for ATX inhibition and indicate pathways for their optimization. These studies suggest that ATX is an approachable molecular target for the rational design of chemotherapeutic agents directed against human malignancies driven by the ATX / LPA axis, especially including malignant melanoma, among numerous others including breast and ovarian cancers.

The invention provides a preparation method of a multifunctional electrospinning scaffold for bone regeneration. The method belongs to the field of tissue engineering and regenerative medicine, and comprises the following specific steps: 1, preparing lysophosphatidic acid nanoparticles and deferoxamine nanoparticles; 2, preparing a medical polymer mixed solution and dividing the medical polymer mixed solution into two equal parts; 3, preparing a coaxial electrospinning shell layer solution from the lysophosphatidic acid nanoparticles, zinc oxide nanoparticles and the medical polymer mixed solution; 4, preparing a coaxial electrospinning core layer solution from the deferoxamine nanoparticles and another medical polymer mixed solution; 5, preparing the electrospinning scaffold with a core-shell structure; and 6, performing vacuum drying until a solvent is removed, thereby finally obtaining the product. According to the prepared multifunctional electrospinning scaffold for bone regeneration, the lysophosphatidic acid nanoparticles and the zinc oxide nanoparticles are arranged on the shell layer, the deferoxamine nanoparticles are arranged on the core layer, and the electrospinning scaffold has the functions of resisting bacteria, promoting osteogenesis, promoting revascularization and the like at the same time and can be used for repairing bone tissues in a complex microenvironment.

The invention discloses a composition for promoting coloring and sweetening of grapes. The composition comprises a component A (natural abscisic acid), a component B (monopotassium phosphate), a component C (one of lysophosphatidyl ethanolamine, lysophosphatidic acid and lysophosphatidyl choline) and a component D (one of sodium benzoate, levulinic acid and glutamic acid). A weight ratio of the component A to the component B to the component C to the component D is 1:6-30:0.04-2:1; the component A, the component B, the component C and the component D which are completely dissolved in absolute ethyl alcohol are mixed with distribution agents and water to obtain solution, and each liter of solution comprises 5-25 grams of component A, 30-150 grams of component B, 1-10 grams of component C, 5-25 grams of component D and 5 mL of distribution agents. The composition has the advantages that coloring of fruits can be obviously shifted to an earlier time after the composition is applied to the grapes, and the fruits are good in coloring uniformity; the sugar degree of the fruits can be upgraded, the sugar-acid ratios can be increased, and accordingly the quality can be improved; deterioration of the hardness of the fruits can be prevented after the composition is used, and accordingly the storage and transport quality of the grapes can be improved; the composition is safe and efficient in use.