The invention discloses a method for building a
dairy cattle breast
acinus lactation model
in vitro, and relates to a method for building a
lactation model
in vitro. The method solves the problem of low authenticity of in-vivo environment caused by taking a mammary epithelial
cell as a research carrier in the existing in-vitro study of the
lactation function of the
dairy cattle breast. The method comprises the following steps: 1, preparing a
tissue culture plate of matrix gel; 2, digesting primary cultured
dairy cattle mammary epithelial
cell gestated for 4 months by adopting pancreatin and EDTA, and collecting cells after centrifugalization; 3, preparing
cell suspension and diluting; and 4, adding the cell suspension into the
tissue culture plate of the matrix gel, culturing for 15 days in an
incubator to finish the building. The artificial
building process of the dairy cattle breast
acinus lactation model built by the invention is a
simulation process of in-vitro
simulation of a galactemia protective screen; and a three-dimensional
acinus structure thereof can truly simulate in-vivo situation, has the same lactation function and biological characteristics as the breast acinus in a
ruminant, and has high authenticity of in-vivo environment
simulation.