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Application of α-lactose in the preparation of medicine for preventing and treating acute pancreatitis

A technology for acute pancreatitis and lactose, applied in the field of medicine, can solve problems such as the pathogenesis is not completely clear

Active Publication Date: 2020-01-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Acute pancreatitis is one of the common digestive diseases, and its specific pathogenesis has not been fully clarified

Method used

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  • Application of α-lactose in the preparation of medicine for preventing and treating acute pancreatitis
  • Application of α-lactose in the preparation of medicine for preventing and treating acute pancreatitis
  • Application of α-lactose in the preparation of medicine for preventing and treating acute pancreatitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Establishment of Acute Pancreatitis Model

[0021] Eight-week-old female BALB / C mice were randomly divided into control group (CON), acute pancreatitis group (AP), α-lactose prevention group (Pro) and α-lactose treatment group (The). The mice in the control group were injected with normal saline (0.85%), the injection volume was 200 μL / 20g mouse weight, and the mice in the acute pancreatitis group, α-lactose prevention group, and α-lactose treatment group were injected with cerulein by intraperitoneal injection. The injection volume was 50 μg / kg; normal saline and cerulein were injected once every hour, and 10 consecutive injections were performed. The prevention group drank 100 mg / kg α-lactose aqueous solution three days before the injection of cerulein and intraperitoneally injected 100 mg / kg α-lactose solution 0.5 h before the first injection of cerulein, and the concentration of α-lactose solution was about 10 mg / mL; the treatment group was intraperitonea...

Embodiment 2

[0022] Example 2 α-Lactose reduces serum amylase (AMY) levels during acute pancreatitis

[0023] Amylase can hydrolyze starch to produce glucose, maltose and dextrin. When the substrate concentration is known and excessive, add iodine solution and unhydrolyzed starch to form a blue complex. According to the depth of blue, the hydrolyzed starch can be deduced amount, thus calculating the vitality of AMY. Amylase activity in mouse serum was determined by serum amylase kit, figure 1 The results showed that α-lactose could significantly reduce the level of serum amylase in mice with acute pancreatitis.

Embodiment 3

[0024] Example 3 α-lactose resists the expression of MPO in pancreas tissue during the course of acute pancreatitis

[0025] Myeloperoxidase exists in neutrophils, and the amount of enzyme contained in each cell is certain, accounting for about 5% of the dry weight of the cell. This enzyme has the ability to reduce hydrogen peroxide. Using this feature, the enzyme can be analyzed activity, and to determine the number of neutrophils. The principle is as follows:

[0026] MPO+H 2 o 2 complex; complex + AH 2 (hydrogen donor) H 2 O+MPO+A product

[0027] The yellow compound is generated after the hydrogen donor is o-anisidine, and the amount of A product is measured by colorimetry at 460nm, so as to calculate the activity of MPO and H 2 o 2 Reduced volume and number of white blood cells. The level of myeloperoxide oxidase (MPO) in mouse pancreas tissue was determined by myeloperoxidase kit, figure 1 The results showed that the expression of MPO in the α-lactose treatm...

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Abstract

The invention discloses the application of alpha-lactose for preparing health care foods capable of preventing and treating acute pancreatitis, and belongs to the field of health care foods. The alpha-lactose can reduce pancreatic tissue oedema, serum amylase, and the level of pancreatic tissue medulla peroxidase MPO so as to reduce an inflammatory reaction in an acute pancreatitis process; besides, the alpha-lactose can reduce inflammation mediators in pancreatic tissues and pancreatic acinar cells, such as an MCP-1 level and a TNF- alpha level. In addition, the alpha -lactose has the effect of restraining the activation of signal routes for regulating protein kinase (ERK1 / 2MAPK) and nuclear factors KB (NF-KB) protein outside cells, reduces the soaking of immunocyte, such as neutrophilic granulocyte and macrophage, in pancreatic tissues, has a notable protective effect on acute pancreatitis, and can be used for preparing health care foods capable of preventing and treating acute pancreatitis.

Description

technical field [0001] The invention relates to the application of alpha-lactose in the preparation of medicines for preventing and treating acute pancreatitis, which belongs to the field of medicine. Background technique [0002] Lactose is a disaccharide formed by the combination of one molecule of glucose and one molecule of fructose through glycosidic bonds. It mainly exists in mammalian milk and is one of the necessary components to maintain the nutritional health of infants and young children. Studies have shown that lactose can induce the expression of antimicrobial peptide LL-37 in epithelial cells, revealing the immunomodulatory function of lactose. In addition, a diet low in fermentable oligosaccharides, disaccharides, and polyols can effectively reduce functional gastrointestinal symptoms in patients with irritable bowel syndrome, which shows that lactose can also regulate inflammation while providing nutrition. [0003] Acute pancreatitis is one of the common di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/125A23L33/17A23L33/19A23L7/10A23L21/25A23L29/30A61K31/7016A61P1/18
CPCA23V2002/00A61K31/7016A23V2200/32A23V2250/5118A23V2250/54A23V2250/54252A23V2250/612
Inventor 孙嘉邓园园陈永泉何钊何月
Owner JIANGNAN UNIV
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