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111 results about "Exocytosis" patented technology

Exocytosis (/ˌɛksoʊsaɪˈtoʊsɪs/) is a form of active transport and bulk transport in which a cell transports molecules (e.g., neurotransmitters and proteins) out of the cell (exo- + cytosis) by secreting them through an energy-dependent process. Exocytosis and its counterpart, endocytosis, are used by all cells because most chemical substances important to them are large polar molecules that cannot pass through the hydrophobic portion of the cell membrane by passive means. Exocytosis is in process a large amount of molecules are released thus making it a form of bulk transport.

Exosome-encapsulated nano drug-loading system for tumor treatment and preparation thereof

ActiveCN108543074AIngestion behaviorExcellent killing effectOrganic active ingredientsInorganic non-active ingredientsExocytosisTumor targeting
The invention discloses an exosome-encapsulated nano drug-loading system for tumor treatment, and preparation thereof. The system is obtained by utilizing cell endocytosis of a drug-loading nanometermaterial and then exocytosis. The drug-loading nanometer material is loaded with an antitumor medicine including at least one of a chemotherapeutic, a medicine used for immunotherapy and a medicine used for modifying a tumor microenvironment. The composition, structure and the like of the key outer component biofilm encapsulating the nano drug-loading system are improved, and compared with the prior art, the exosome-encapsulated nano drug-loading system provides a novel route for biofilm-based biological processed nanoparticles. By utilization of the exosome-encapsulated nano drug-loading system, the composition and the structure of the exosome can be maintained greatly, and the obtained exosome-encapsulated nano drug-loading system has good stability and tumor targeting performance duringblood circulation. .
Owner:HUAZHONG UNIV OF SCI & TECH

Functional albumin and preparation method of nano preparation of functional albumin

ActiveCN105288647AThe synthesis method is simpleChange isoelectric point to basicPowder deliveryOvalbuminExocytosisDrug release
The invention discloses functional albumin and a preparation method of a nano preparation of the functional albumin. The nano preparation of the functional albumin consists of the functional albumin, metal ions and a drug; the metal ions can simultaneously form coordination bonds with the functional albumin and the drug, and can form nanoparticles through induced self-assembly. The nano preparation, through an endocytosis mediated by an albumin receptor (SPARC) on the surface of tumor cells, can deliver the drug into the drug-resistant tumor cells, so as to effectively avoid the exocytosis effect of a p-gp pump on the drug, and then as the coordination bonds break in an acid tumor cell environment through a pH responsibility, the drug releases in cytoplast, enters cell nucleus and inlays in DNA so as to inhibit the synthesis of nucleic acid; and therefore, the growth of the tumor cells is inhibited. Through in vitro characterization, the nano preparation can achieve the relatively good pH responsibility; and through activity evaluation on a cellular level, the system is capable of effectively delivering the drug into the cells, so as to achieve relatively good pH responsive release.
Owner:CHINA PHARM UNIV

Method of inhibiting leukocytes with human cxc chemokine receptor 3 antibody

The present invention relates to proteins or polypeptides, referred to herein as isolated and / Or recombinant mammalian (e.g., human) IP-10 / Mig receptor proteins designated CXC Chemokine Receptor 3 (CXCR3) and variants thereof, including those characterized by selective binding of one or more chemokines (e.g., IP-10 and / or Mig), and / or the ability to induce a cellular response (e.g., chemotaxis, exocytosis). Antibodies reactive with CXCR3 receptors can be produced using the proteins or variants thereof or host cells comprising same as immunogen.
Owner:THEODOR KOCHER INST +1

Alkaline pectic enzyme producing engineering strain and its construction and method for producing alkaline pectic enzyme with the same

An engineering bacterium of producing alkaline pectinase, a construction thereof and a method of producing alkaline pectinase by using the alkaline pertain to the technical field of bioengineering. The invention uses nonpathogenic bacillus sp WSHB04-02 genes group as a model and adopts amplification technology of PCR. And then the sequence pel containing 1.2kb coding alkaline pectinase genes section is obtained. At the same time from the point of the demand for industrial production, the invention makes use of a shuttle expression carrier to restructure yeast Pichiapastoris(pel), which means to produce engineering bacterium CGMCC No.2143 of alkaline pectinase (E.C.4.2.2.2). The invention also provides CGMCC No.2143 as the method of producing alkaline pectinase for fermented yeast strain. The genes engineering bacterium aims at exocytosis of protein and the species of extracellular protein is few, which simplifies the purification technique of producing alkaline pectinase by using microorganism and reduces the cost and shoetens the time for ferment. Besides, the invention improves the production efficiency and lays a foundation for industrialization of the ferment method of producing alkaline pectinase by using microorganism.
Owner:JIANGNAN UNIV

Targeting carbon nanotube drug delivery system, and preparation method and applications thereof

The invention provides a targeting carbon nanotube drug delivery system, and a preparation method and applications thereof. The drug delivery system comprises a conjugate and a drug carrying carbon nanotube that is combined with the conjugate in a non-covalent mode; wherein the conjugate comprises a modifying material that can enhance the water solubility and biocompatibility of the drug delivery system and a targeting molecule that is combined with the modifying material in a covalent mode; and the drug carrying carbon nanotube is composed of carbon nanotubes and drug molecules that adsorb on the surface of the carbon nanotubes. One step modification technology is adopted, the problems on purification and yield caused by multi-step modification are avoided; at the same time, the carbon nanotube and drug delivery system are enable to have water solubility, biocompatibility, and multi-functions; the exocytosis of drug resistant tumor cells on drug molecules is effectively overcome, a novel strategy is provided for overcoming multi-drug resistance of tumor cells, and thus the provided drug delivery system has a wide application prospect in the field of drug resistant tumor treatment.
Owner:INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A

Construction method of corynebacterium glutamicum engineering bacteria for high production of L-lysine

The invention relates to a construction method of corynebacterium glutamicum engineering bacteria for high production of L-lysine. According to the method, the engineering bacteria are constructed by inactivating an NCgl1221 gene of corynebacterium glutamicum, wherein the nucleotide sequence of an NCgl1221 gene encoding frame is shown as SEQ ID NO. 1. The invention provides a construction method of a corynebacterium glutamicum strain for high production of the L-lysine; the constructed strain can obviously decrease glutamicum exocytosis due to the inactivation of the endogenous gene NCgl1221; compared with a wild strain, the strain can be used for producing high-concentration L-lysine and can effectively reduce the content of glutamicum which is a byproduct of a fermentation liquor; and the strain, as an L-lysine production strain, can further reduce production cost and purification cost.
Owner:QILU UNIV OF TECH +2

Novel ultrasensitive cell based sensors and uses thereof

The present invention relates to a novel cell based sensor useful for drug discovery that comprises a cell line with professional regulated exocytosis of secretory granules transfected with a protease as a reporter polypeptide stored in the regulated secretory granules of the cell line with professional regulated exocytosis and having either an endogenous or a heterologous molecule as a modulator of regulated secretory granules exocytosis, such said granule stored protease reporter having at least: a high resistance to conditions already present inside the granules such as low pH and proteolysis by other proteases; enzymatic activity after exocytosis; a highly specific cleavage sequence; a very low level of secretion under unstimulated or basal conditions; and a high signal to background activity in a media compatible with cell culture viability and granule exocytosis for a high throughput robust and sensitive detection.
Owner:CANVAX BIOTECH
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