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Method for overcoming reverse tolerance of tumors

An anti-tumor drug and tumor technology, applied in the field of biomedicine, can solve the problems of difficult to achieve effective reversal concentration, low drug utilization rate, poor targeting, etc., and achieve the effects of simple and easy acquisition, simple nutritional requirements, and easy control.

Active Publication Date: 2020-02-07
SHENZHEN INST OF ADVANCED TECH
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Problems solved by technology

However, in further research, it is found that these reversal inhibitors are difficult to achieve the effective reversal concentration of in vitro experiments in vivo experiments, and the drug utilization rate is low; more importantly, due to poor targeting, reversal inhibitors will also interact with P-glycoprotein in healthy tissues. effect, causing the drug to produce toxic side effects on healthy tissues, so it cannot be put into clinical use

Method used

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  • Method for overcoming reverse tolerance of tumors
  • Method for overcoming reverse tolerance of tumors

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Experimental program
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Embodiment

[0029] Prepare for the experiment: Prepare a 500ml Erlenmeyer flask, pour cyanobacteria and sterilized BG11 medium into the aseptic operating table, seal the bottle mouth with a breathable parafilm, and place it in the sun for cultivation and amplification.

[0030] Experiment 1: Increased oxygenation in vitro to increase the entry of doxorubicin into cells

[0031]Spread hypoxic breast cancer cells (MCF-7) and normal state breast cancer cells (MCF-7) into the confocal dish and put them into the incubator (the hypoxic cells are placed in the hypoxic incubator, the normal state The cells were cultured in a normal incubator) for 24 hours, and the cells in the hypoxic state were added with 2×10 7 cfu / mL cyanobacteria or no cyanobacteria, 660nm laser irradiation or no laser irradiation and culture for 24 hours, add DOX for co-incubation for 1 hour, stain the nucleus, and shoot confocal.

[0032] Confocal results such as figure 1 As shown, the cyanobacteria and laser irradiation ...

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Abstract

The invention discloses a new function of cyanobacteria. Under irradiation of specific wave length lasers, and through photosynthesis, oxygen is generated, the condition of oxygen deficiency caused bytumors can be improved, and the problem of reverse tolerance of chemicals, such as adriamycin can be effectively solved. The cyanobacteria is subjected to photosynthesis in light to generate oxygen,and under the aerobic condition, hypoxia factors are reduced for tumor cells, so that multidrug resistance genes can be restrained, expression of P-glycoprotein is restrained to restrain exocytosis ofcancer cells on adriamycin, the reverse tolerance of the cancer cells on the reverse tolerance is overcome, and the antitumor treatment effect can be increased.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for overcoming tumor drug resistance. Background technique [0002] Chemotherapy is one of the main methods for treating malignant tumors. The biggest advantage of chemotherapy compared with surgery and radiotherapy is that chemotherapy is a systemic treatment. Chemotherapy drugs can reach the whole body through the human circulatory system through oral administration, injection, etc., so they have a killing effect on primary cancer lesions, metastatic lesions, and even free circulating tumor cells. Through natural extraction, artificial synthesis and a large number of screening, many drugs have been approved by the US Food and Drug Administration for clinical application. At present, common chemotherapy drugs include adriamycin, 5-fluorouracil, camptothecin, paclitaxel, and etoposide. , Cismolybdenum, etc. However, while chemotherapy drugs kill tumor cells, they...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/748A61K45/06A61K31/704A61P35/00A61P35/04C12Q1/02
CPCA61K31/704A61K35/748A61K45/06A61P35/00A61P35/04G01N33/5011A61K2300/00
Inventor 蔡林涛何华美刘兰兰梁锐晶周海梅潘宏
Owner SHENZHEN INST OF ADVANCED TECH
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