The invention discloses a
long chain noncoding
RNA marker for auxiliary diagnosis of
colorectal cancer (CRC).
Long chain noncoding
RNA is
HOTAIR; auxiliary diagnosis or prediction of patient conditiondevelopment of the CRC can be realized by detecting expression quantity of the
long chain noncoding
RNA; differentiation, infiltration depth, proliferation and
metastasis conditions of the CRC can bepredicted by monitoring expression conditions of p21 and a value of judging prognosis is realized. Expression level of LncRNA
HOTAIR gene can be detected by RT-qPCR, real-time quantitative PCR, immunodetection, in-situ hybridization,
chip or a high-
throughput sequencing platform; meanwhile, the invention also discloses silencing
HOTAIR which can obviously inhibit the proliferation, migration andinvasion abilities of CRC cells, reduce the expression of PCNA, Ki67,
cyclin E and CDK2 and medicate expression increase of p21. The invention provides a
molecular marker for the CRC and provides theoretical basis for mechanism research and clinical individualized therapy of the CRC; a brand-new
treatment strategy can be provided for clinically treating the CRC by monitoring the expression of theLncRNA HOTAIR in the CRC.