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Application of salvianolic acid A in preparing anti-tumor metastasis medicine

An anti-tumor metastasis, salvianolic acid technology, applied in anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve the problems of cell damage, patient death, inability to effectively inhibit tumor cell metastasis, etc., and achieve a good selective effect

Active Publication Date: 2011-04-27
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cytotoxic drugs often cause damage to normal cells while killing tumor cells, and cannot effectively inhibit tumor cell metastasis. In clinical practice, the primary tumor lesion is alleviated, but multiple metastases appear, which eventually leads to disease progression
In recent years, although the detection and treatment of malignant tumors have made great progress, tumor metastasis is still the leading cause of cancer treatment failure and patient death.

Method used

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  • Application of salvianolic acid A in preparing anti-tumor metastasis medicine
  • Application of salvianolic acid A in preparing anti-tumor metastasis medicine
  • Application of salvianolic acid A in preparing anti-tumor metastasis medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: The effect of salvianolic acid A on the proliferation of HPMEC, LTEP and A549 cells was detected by CCK-8 method.

[0023] Human lung microvascular endothelial cells HPMEC, human lung adenocarcinoma cell LTEP, and human lung adenocarcinoma cell A549 were cultured in vitro, and different concentrations (0-80 μg / ml) of salvianolic acid A were added to detect HPMEC, LTEP, and A549 cells by 24hCCK-8 method Proliferation inhibition, which shows that salvianolic acid A has a strong dose-dependent inhibitory effect on lung cancer cells LTEP and A549, but has no obvious inhibitory effect on normal cell HPMEC. The results are shown in figure 1 .

Embodiment 2

[0024] Example 2: Transwell cell migration assay was used to determine the effect of salvianolic acid A on cell migration ability.

[0025] Will 5X10 4 The A549 and LTEP cells were inoculated in the Transwell chamber and continued to culture. After the cells adhered to the wall, DMEM complete medium containing different concentrations of salvianolic acid A was added to the upper chamber, and DMEM complete medium containing 20% ​​FBS was added to the lower chamber, and culture was continued for 24 hours. The unmigrated cells on the upper surface of the membrane were carefully wiped off with a cotton swab, and the cells that migrated to the lower surface of the membrane were fixed with 4% paraformaldehyde and stained with Giemsa. Three high-power fields of view were randomly selected to count the number of migrating cells. The experiment was repeated three times. Cell migration rate = [the average number of cells in the experimental group / the average number of cells in the bl...

Embodiment 3

[0028] Example 3: The damage repair experiment detects the effect of salvianolic acid A on the migration ability of tumor cells.

[0029] Take A549 and LTEP cells in the logarithmic growth phase, and add 5×10 cells to each well of a 24-well plate 4 After the cells grow into a single layer, draw a "one"-shaped scratch, wash with PBS, add different concentrations of salvianolic acid A to the control group and the experimental group, and take pictures. After 22 hours of culture, the same part is photographed again to measure the migration distance. The experiment was repeated 3 times, and the cell migration rate=[(0h average value of the experimental group-22h average value of the experimental group) / 0h average value of the blank control group-22h average value of the blank control group]×100%.

[0030] Salvianolic acid A inhibited the migration of LTEP and A549 cells at different concentrations (0-40 μg / ml) for 22 hours. The results showed that salvianolic acid A had a dose-depe...

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Abstract

The invention discloses an application of salvianolic acid A in preparing an anti-tumor metastasis medicine. The invention has the importance of discovering that the salvianolic acid A in salvia miltiorrhiza has notable inhibiting effect on tumor metastasis, has obvious influence on the tumor metastasis related gene and protein, can selectively inhibit proliferation of tumor cells, and has safety and low toxicity to normal cells of the human body, so that the salvianolic acid A has application prospects in preparing the anti-tumor metastasis medicine.

Description

technical field [0001] The invention relates to a new application of salvianolic acid A, in particular to the application of salvianolic acid A in the preparation of anti-tumor metastasis drugs. Background technique [0002] Tumor metastasis is the main cause of malignant tumor treatment failure and patient death, and metastasis is the basic biological characteristic of malignant tumors. Tumor metastasis is a multi-step, multi-stage, multi-pathway, and a series of complex processes involving multi-gene changes, including tumor cells shedding from the primary tumor, invading blood vessels or lymphatic vessels, migrating, and adhering to appropriate sites to induce tumor angiogenesis, and to resist tumor metastasis. Host anti-tumor immunity eventually forms distant metastases. Studies have shown that not only a variety of genes are involved in the process of tumor cell invasion and metastasis, but also the joint action and regulation of some related factors, enzymes and prote...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/216A61P35/04A61P11/00
Inventor 陈卫平毕蕾曾莉王昕
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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