The invention discloses a deer blood health medicinal liquor with functions of resisting senescence and improving memory and a preparation method thereof. The medicinal liquor comprises deer blood, corn cervi pantotrichum, semen juglandis, ginkgo fruit, radix notoginseng powder, rhizoma gastrodiae, acanthopanax root, herba boschniakiae rossicae, longan pulp, poria cocos, fructus lycii, radix ginseng, radix codonopsis, radix polygoni multiflori, rhizoma curculiginis, radix astragali, semen astragali complanati, lucid ganoderma, rhizoma dioscoreae, rhizoma atractylodis macrocephalae, rhizoma polygonati, winterworm summer herb, Chinese angelica and rhizoma chuanxiong. The medicinal liquor is novel deer blood health medicinal liquor with simple preparation and good taste; the alcohol content of the medicinal liquor is 38%-52%; the medicinal liquor is free of chemical components and has heath functions of resisting senescence and improving memory; the preparation method of the medicinal liquor is scientific and advanced; the preparation process is simple; the extraction is carried out by a wine leaching process and a process of extracting by decocting with water; various effective ingredients in the raw materials can be effectively extracted; the impurities are removed; and the wine leaching process adopts a two-step extraction method and is capable of fully extracting effective ingredients of part of the components.