Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus with efficacy of preventing and treating diabetic encephalopathy and separation method and application thereof

A technology for diabetic encephalopathy and separation method, applied in the field of Lactobacillus and its separation, can solve the problem of less diabetic encephalopathy, and achieve the effects of improving the content of nitric oxide, reducing the content of MDA, and improving the activity of SOD

Active Publication Date: 2020-02-11
JIANGSU WECARE BIOTECHNOLOGY CO LTD
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The important role of intestinal flora in systemic diseases has been paid more and more attention by researchers, but there are few studies on the prevention and treatment of diabetic encephalopathy through probiotics

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus with efficacy of preventing and treating diabetic encephalopathy and separation method and application thereof
  • Lactobacillus with efficacy of preventing and treating diabetic encephalopathy and separation method and application thereof
  • Lactobacillus with efficacy of preventing and treating diabetic encephalopathy and separation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Isolation and identification of Lactobacillus paracasei.

[0041] The specific method is: take Qinghai old yoghurt (product model 57338258454) as an isolated sample, dilute it, spread it on a sterile MRS medium with a coating rod, and carry out anaerobic culture at 37°C for 48 hours to obtain colonies; pick the morphology The regular colony was cultured by streaking five times on the sterile MRS medium to separate and purify, and a single pure colony was obtained, which was determined to be Lactobacillus by microscopic examination, and the 16S rDNA sequence homology analysis method was used to screen out the said anti-diabetic effect. Lactobacillus for encephalopathy efficacy. The formula of the MRS medium is: casein peptone 10.0g / L, beef extract 10.0g / L, yeast extract 5.0g / L, glucose 5.0g / L, sodium acetate 5.0g / L, diamine citrate 2.0 g / L, Tween-80 1.0g / L, dipotassium hydrogen phosphate 2.0g / L, magnesium sulfate heptahydrate 0.2g / L, manganese sulfate heptahydrate 0.05g...

Embodiment 2

[0050] Preparation of Lactobacillus paracasei bacteria powder.

[0051] The specific method is as follows: pick a single colony of Lactobacillus paracasei LC86, inoculate it in 50mL MRS medium, pH 6.8, anaerobic culture at 37°C for 24 hours, and obtain the seed liquid; inoculate the seed liquid with 2% inoculation amount into a new MRS In the culture medium, 37°C, anaerobic culture for 24 hours, to obtain the fermentation broth; centrifuge the fermentation broth at 12000rpm, 10min, remove the fermentation supernatant, and isolate the bacteria; freeze-dry the bacteria to obtain Lactobacillus paracasei pink.

Embodiment 3

[0053] Animal experimental research on prevention and treatment of diabetic encephalopathy.

[0054] 1. Experimental materials

[0055] 1. Lactobacillus paracasei LC86 bacteria powder, the number of viable bacteria is 1×10 8 CFU / g, refer to Example 2 for preparation.

[0056] 2. STZ solution: Weigh 100mg of streptozotocin, add 5mL of citric acid buffer, dissolve the streptozotocin in an ice bath, and use it within 30min.

[0057] 3. Experimental animals: Wistar rats, male, 12 weeks old, weighing 230-250 g.

[0058] 2. Preparation of diabetic encephalopathy rat model

[0059] Rats were adaptively fed for one week, and the Y-maze was used to select rats with similar learning and memory abilities to establish a model of diabetic encephalopathy by taking advantage of the physiological characteristics of preferring darkness and avoiding light. Wistar male rats were fasted for 12 hours, and STZ solution was injected intraperitoneally at 60 mg / Kg. According to the habitual model...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a Lactobacillus with the efficacy of preventing and treating diabetic encephalopathy and a separation method and application thereof. The Lactobacillus is named as a Lactobacillus paracasei LC86 strain, and the preservation unit is China General Microbiological Culture Collection Center, the preservation time is December, 2013, the preservation number is CGMCC 1.12731, andthe address is No. 3, No. 1 Courtyard, Beichenxi Road, Chaoyang District, Beijing City. According to the Lactobacillus, the increase of the content of nitric oxide caused by diabetic encephalopathy can be obviously improved, the antioxidant enzyme activity of brain tissues can be improved, and the learning and memory ability can be improved. The Lactobacillus is applied to medicines for preventing and treating diabetic encephalopathy, has no toxic or side effect, and can be used for treating clinical patients together with other nutrients.

Description

technical field [0001] The invention belongs to the field of chemical medicine, and in particular relates to a lactobacillus and its isolation method and application. In particular, it relates to a lactobacillus with the effect of preventing and treating diabetic encephalopathy and its isolation method and application. Background technique [0002] Diabetes has a high incidence rate, many complications, high mortality rate, and high disability rate, most of which are type Ⅱ diabetes. Long-term elevated blood sugar in diabetic patients without control can lead to various chronic complications, such as coronary heart disease, hypertension, myocardial infarction, stroke, Alzheimer's disease, Parkinson's disease, kidney disease, retinopathy, etc. Diabetic complications have become the main cause of death and disability in diabetic patients, including diabetic encephalopathy, which obviously has a serious impact on the quality of life of diabetic patients. [0003] Diabetic enc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A61K35/747A61P3/10A61P25/00C12R1/225
CPCC12N1/20C12N1/02A61K35/747A61P3/10A61P25/00C12R2001/225C12N1/205
Inventor 方曙光郝沛妍朱建国陈珂可于惠程文蒋大成
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com