The invention discloses an siRNA, an siRNA
plasmid and a
lentivirus for targeted inhibition of EGFL9
gene expression, as well as a construction method of the
lentivirus, and application of the SiRNA,the siRNA
plasmid and the
lentivirus in preparation of
liver cancer treatment drugs, and provides an action target of the EGFL9
gene for
liver cancer treatment by researching the expression level of the EGFL9 in
liver cancer tissues. An siRNA sequence for targeted inhibition of expression of the EGFL9
gene, and corresponding siRNA expression plasmids and siRNA lentiviruses are designed; therefore,the expression of the EGFL9 gene in liver
cancer cells can be efficiently inhibited, the
invasion and migration capability of the liver
cancer cells can be effectively inhibited, and the EGFL9 gene targeted therapeutic
drug is of great significance to the preparation of the EGFL9 gene targeted therapeutic
drug for liver
cancer; meanwhile, the method can be used for developing corresponding targeted therapeutic drugs such as RNAi,
monoclonal antibodies and
small molecule antagonists, so that an
effective method is provided for developing targeted therapeutic drugs for treating liver cancer byaiming at the EGFL9.