150 results about "Tumor stem cell" patented technology

Disclosed are methods for isolating cell populations enriched in tumor stem cells (cancer stem cells), and isolated cell populations substantially enriched in cancer stem cells that are tumorigenic in vivo. Also provided are new methods of tumor diagnosis and classification and personalized methods of treatment for subjects with tumors, based on the availability of populations of cancer stem cells derived from the subject's tumor using the disclosed methods.

The invention discloses a reagent for detection of an ROR1 protein in circulating blood and an application thereof, and particularly discloses the reagent for the detection of the ROR1 protein of circulating tumor cells in peripheral blood. The reagent includes an ROR1 antibody, a buffer solution, a marker, a nuclear dye and a lymphocyte identification antibody. A characteristic that ROR1 is only expressed in cancer cells and is not expressed in normal adult tissue cells is utilized, and the reagent is applied to detect the expressed ROR1 protein of the circulating tumor cells or tumor stem cells in the circulating blood, wherein the circulating tumor cells or the tumor stem cells fall off from a primary tumor nidus into the blood; limitations of a traditional method are overcome, and the accuracy and the reliability of the detection of the circulating tumor cells are greatly improved. The reagent can be used for helping detect the tumor cells in the circulating blood of a tumor patient, and is a potential breakthrough point for tumor disease diagnosis, evaluation of therapeutic effects and cancer metastasis monitoring.

Arrangements are provided for destroying tumor cells. According to one aspect, an arrangement includes a device having at least one high-frequency ultrasound generator for generating a thermally active, high-frequency oscillation, a plurality of first low-frequency ultrasound generators for generating a therapeutically active low-frequency oscillation, wherein the first low-frequency ultrasound generators each generate a different frequency, and a control system connected to the high-frequency ultrasound generator and to the first low-frequency ultrasound generators in such a way that the tumor cells are subjected to a high-frequency oscillation acting thermally on the tumor cells and to a low-frequency oscillation. The arrangement further includes a biopsy device having a plurality of individual receivers for tissue samples and a plurality of second low-frequency ultrasound generators operatively connected to the individual receivers for subjecting the tissue samples to an oscillation, wherein oscillations with the same frequency can be generated respectively by first and second low-frequency ultrasound generators.

The invention provides a composition for stimulating an immune response in a patient having different types of cancer including those having adenocarcinoma comprising a combination of allogeneic tumor cells and / or tumor stem cells that are selected on the basis of secreting at least one immunosuppressive agent, e.g., TGF-β, and that are genetically modified to reduce or inhibit the expression of said at least one immunosuppressive agent, e.g., TGF-β, and that collectively express a spectrum of tumor associated antigens representative of colon, breast, lung, prostate, pancreas, kidney, endometrium, cervix, ovary, thyroid, and other glandular tissue carcinomas, as well as squamous, melanoma, central nervous system, and lymphomas and a physiologically acceptable carrier. The adenocarcinoma can be, for example, colon, breast, lung, prostate, pancreas, kidney, endometrium, cervix, ovary, thyroid, or other glandular tissue, as well as squamous, melanoma, central nervous system, and lymphomas. The invention also provides a composition containing said combination of allogeneic tumor cells and or tumor stem cells and an allogeneic cell expressing a cytokme or an antibody that inhibits an immune suppressive molecule.

A method and composition for hyperthermally treating tumor cells in a patient under conditions that affect tumor stem cells and tumor cells. In one embodiment, the method provides a synergistic effect with chemotherapy.

The embodiment of the invention provides a device and method for targeted ablation of cells, based on a pulsed electric field, a medium and an electronic device. The method includes the steps: generating a high-voltage electric pulse targeting the ablation target cells; applying a high voltage electric pulse to a target cell, wherein a waveform of the high voltage electric pulse and an electric field intensity generated by the high voltage electric pulse are configured according to a component structure parameter and a dielectric parameter of the target cell. The technical proposal of the embodiments of the present invention can repeatedly generate high-voltage electric pulses targeting on tumor stem cells to induce their pyrogenation, and is applied to the tumor stem cells and the escapedhigh-malignancy tumor cells by irreversible electroporation ablation through electrodes and induce their pyrogenation.

The invention relates to an environmental response type anti-tumor nanometer medicine with high medicine loading capacity, a carrier and a preparation method thereof. The nanometer medicine comprisesan amphiphilic polymer carrier and a medicine prodrug; the amphiphilic polymer carrier comprises a hydrophilic segment and a hydrophobic segment which are connected mutually; the hydrophilic segment comprises polyethylene glycol or poly(N-(2-hydroxypropyl)methacrylamide); the hydrophobic segment comprises D-alpha-tocopherol succinate; the medicine prodrug comprises a medicine for treating tumor aswell as a hydrophobic chain segment which is connected with the medicine through a chemical bond; and the hydrophobic chain segment comprises D-alpha-tocopherol succinate. The nanometer medicine hasthe characteristics of high medicine loading capacity, environmental responsiveness and adjustable and controllable grain size, and can achieve the properties that the tumor penetration ability is high, the tumor cell multidrug resistance is achieved, drug-resistant cells are killed in advance, tumor related fibroblast is not killed and tumor stem cells can be killed effectively according to composition of different polymers and the loaded prodrug molecule types.

The invention relates to an animal model establishment method of human primary skin squamous epithelial cell cancer stem cell. The method comprises the following steps of: preparing collected skin squamous epithelial cell cancer tissue in a clinical case into a single-cell suspension, separating CD31-CD24-CD45-CD61-CD133+tumor stem cell population, suspending and mixing with 1*10<6> human primary mouse embroy fibroblasts and 1*10<6> human primary endothelial cells in matrigel, and then injecting the obtained product into microenvironment through humanized nude mouse subcutaneous tumorigenesis parts. The invention makes further research on molecular biological mechanisms of human skin primary SCC tumour possible.

The invention discloses application of a bifluorescence reporting system in tumor stem cell targeted drug screening and a method thereof. Red and green fluorescence proteins are utilized to respectively mark colony tumor cells and tumor stem cells, and the targeting properties of the drug for the tumor cells and tumor stem cells can be respectively evaluated by using a high-content imaging system. No targeted drug screening methods for tumor stem cells exist at present. The tumor stem cell screening method established by the invention is hopeful to be used for screening novel antineoplastic drugs for tumor stem cells, thereby enhancing the screening effect of the antineoplastic drugs.

A method and composition for hyperthermally treating tumor cells in a patient under conditions that affect tumor stem cells and tumor cells. In one embodiment, the method is administered in combination with localized internal ionizing radiation therapy.

The invention provides an enriching and screening method for tumor stem cell. The combination of ball culture and immunomagnetic beads separation method is used for enriching and screening the tumor stem cells, the details are as follows: continuously culturing the tumor cells in a no-serum ball culture medium, using the QPCR method to simultaneously examine the relative levels of tumor stem cell markers, when the expression levels of specific marker is relatively the highest, supplement with immunomagnetic and positive, single or double screening tumor stem cells. The combination of the two screening methods can significantly improve such practical problems as low separation rates, low purity levels and the inconveniences of after application for tumor stem cells.

The invention discloses a compound and application thereof. The compound is the compound shown in the formula (I) or a stereoisomer or a pharmaceutical acceptable salt or solvate or a prodrug of the compound shown in the formula (I). The compound can restrain tumor cell proliferation through the effect RRM2, and restrain tumor stem cell regeneration, thereby being effectively used for preparing medicine for preventing or treating proliferative diseases and particularly anti-cancer medicine.

A method and composition for hyperthermally treating tumor cells in a patient under conditions that affect tumor stem cells and tumor cells. In one embodiment, the method is administered in combination with localized internal ionizing radiation therapy.

A method and composition for hyperthermally treating tumor cells in a patient under conditions that affect tumor stem cells and tumor cells. In one embodiment, the method provides a synergetic effect with chemotherapy.