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Animal model establishment method of human primary skin squamous epithelial cell cancer stem cell

A technology of tumor stem cells and squamous epithelium, which can be used in pharmaceutical formulations, emulsion delivery, unknown raw materials, etc. It can solve the problems of low purity of cell populations, specificity problems, and failure to effectively exclude tumor tissue fibroblasts and endothelial cells. , to achieve the effect of promoting growth and differentiation, and increasing the tumorigenic rate

Active Publication Date: 2013-08-14
WUHAN DENUOMEI BIOLOGY MEDICINE TECH
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Problems solved by technology

CD45 isolated from primary squamous cell carcinoma of human skin + CD133 + Although the cells have the characteristics of enriching tumor stem cells, the purity of the cell population is low, and the fibroblasts and endothelial cells in the tumor tissue cannot be effectively excluded, and the gene profile specifically expressed cannot be further studied. The success rate of tumorigenesis in mice is not high. Specificity is also a problem

Method used

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  • Animal model establishment method of human primary skin squamous epithelial cell cancer stem cell
  • Animal model establishment method of human primary skin squamous epithelial cell cancer stem cell
  • Animal model establishment method of human primary skin squamous epithelial cell cancer stem cell

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Embodiment Construction

[0022] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0023] Collect fresh skin squamous cell carcinoma samples from clinical patients, immediately use a scalpel and scissors to mince the tumor into 1-2mm2 fragments as much as possible, and incubate in 200Unit / ml collagenase III and 50U / ml separation enzyme at 37°C for 30 Use GentleMACS Tissue Separator (Miltenyi-Biotec Company) program D to further separate the tissue, centrifuge at 300g for 5 minutes at room temperature, discard the supernatant, and continue to use 5ml of 0.05% trypsin in 37 Digest at ℃ for 5 minutes, and use a 40 μm cell strainer to remove residual tissue, and filter to separate in...

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Abstract

The invention relates to an animal model establishment method of human primary skin squamous epithelial cell cancer stem cell. The method comprises the following steps of: preparing collected skin squamous epithelial cell cancer tissue in a clinical case into a single-cell suspension, separating CD31-CD24-CD45-CD61-CD133+tumor stem cell population, suspending and mixing with 1*10<6> human primary mouse embroy fibroblasts and 1*10<6> human primary endothelial cells in matrigel, and then injecting the obtained product into microenvironment through humanized nude mouse subcutaneous tumorigenesis parts. The invention makes further research on molecular biological mechanisms of human skin primary SCC tumour possible.

Description

technical field [0001] The invention relates to a method for establishing an animal model of tumor stem cells, in particular to a method for establishing an animal model of human primary skin squamous cell carcinoma tumor stem cells. Background technique [0002] Although great progress has been made in the study of molecular-level pathogenesis of tumors and the diagnosis and treatment of cancer, the mortality rate of cancer is still high, and there is still a lack of effective cure. Surgery, radiation therapy, and systemic treatments, such as chemotherapy or hormone therapy, are traditional approaches aimed at eliminating or killing rapidly dividing cancer cells. These methods have clinical limitations. All these traditional treatments are only effective for early-stage or non-metastatic tumors, but helpless for metastatic tumors. [0003] In the past 20 to 30 years, the concept of cancer stem cells (CSC) has been gradually confirmed by more and more researches. It has be...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/36A61K9/10
Inventor 邱启裕全欣鑫
Owner WUHAN DENUOMEI BIOLOGY MEDICINE TECH
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