46 results about "Squamous epithelial cell" patented technology

The invention discloses a vaginal secretion staining fluid, and a preparation method and a staining method thereof. According to the preparation method, a blue basic dyestuff and a red basic dyestuff are mixed so as to obtain the vaginal secretion staining fluid, so that the vaginal secretion staining fluid possesses staining effects of universal staining fluid, staining time can be shortened greatly, staining time is shortened from general 5-30min to 20s, staining efficiency is increased greatly, and screening speed of clinical samples is accelerated. And in addition, imagines obtained via staining with the vaginal secretion staining fluid are clear, and are convenient for identification; surface layer squamous epithelial cells, middle layer epithelial cells, bottom layer epithelial cells, clue cells, leukocytes, erythrocytes, monilia albicans, and trichomonad can be identified clearly; and the vaginal secretion staining fluid is suitable for large-scale sample clinical screening.

The invention relates to an improved cervical squamous epithelial cell staining method, a cervical squamous epithelial cell staining kit and application of the method and the kit to diagnosis of individual cervical squamous epithelial cell cancers. The improved cervical squamous epithelial cell staining method comprises the steps of staining cervical squamous epithelial cells by using cell acid phosphatase staining reagent and staining the cervical squamous epithelial cells by using Pap staining reagent. The cervical squamous epithelial cell staining kit comprises the cell acid phosphatase staining reagent and the improved Pap staining reagent. The invention additionally relates to a kit for diagnosing individual cervical squamous epithelial cell cancers or precancerous lesions.

The invention relates to an animal model establishment method of human primary skin squamous epithelial cell cancer stem cell. The method comprises the following steps of: preparing collected skin squamous epithelial cell cancer tissue in a clinical case into a single-cell suspension, separating CD31-CD24-CD45-CD61-CD133+tumor stem cell population, suspending and mixing with 1*10<6> human primary mouse embroy fibroblasts and 1*10<6> human primary endothelial cells in matrigel, and then injecting the obtained product into microenvironment through humanized nude mouse subcutaneous tumorigenesis parts. The invention makes further research on molecular biological mechanisms of human skin primary SCC tumour possible.

The invention discloses a kit for measuring the content of a squamous cell carcinoma antigen (SCC) in human serum by a magnetic particle chemiluminescence method. The kit comprises a calibrator, a quality control, a resistant reagent, a magnetic particle reagent and a luminescent substrate. The resistant reagent comprises fluorescein isothiocyanate-labeled squamous cell carcinoma antigen-coated antibodies and alkaline phosphatase-labeled squamous cell carcinoma antigen-coated antibodies. The magnetic particle reagent is a connector of magnetic particles and goat anti-FITC. The magnetic particle is used as an immune reaction solid phase. Through combination of a chemiluminescence enzyme-linked immunoassay method and a chemiluminescence measuring instrument, the method is used for detectingthe SCC content of human serum, simultaneously measures multiple samples based on a fully automated chemiluminometer, realizes high-throughput rapid measurement of SCC, and greatly improves accuracy,specificity, precision and detection efficiency.

The present invention relates to novel compounds and pharmaceutical compositions thereof, and methods for inhibiting the activity of SHP2 phosphatase with the compounds and compositions of the invention. The present invention further relates to, but is not limited to, methods for suppressing tumor cell growth, ameliorating the pathogenesis of systemic lupus erythematosus, and the treatment of various other disorders, including Noonan syndrome, diabetes, neutropenia, neuroblastoma, melanoma, juvenile leukemia, juvenile myelomonocytic leukemia, chronic myelomonocytic leukemia, acute myeloid leukemia, and other cancers associated with SHP2 deregulation with the compounds and compositions of the invention, alone or in combination with other treatments. Other cancers associated with SHP2 deregulation include HER2-positive breast cancer, triple-negative breast cancer, ductal carcinoma of the breast, invasive ductal carcinoma of the breast, non-small cell lung cancer, esophageal cancer, gastric cancer, squamous-cell carcinoma of the head and neck (SCCHN), and colon cancer.

The invention provides an establishing method of a Chinese tongue squamous cancer cell line (CTSC-1), and provides a material base for researching the characteristics of tongue squamous cancer and therapeutics thereof. The establishing method comprises the steps of: carrying out primary culture on the tongue squamous cancer cells by adopting a tissue explants adherent method; subculturing when the cell density reaches about 80 percent; carrying out RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection on stably subcultured cells to indicate that the cells express tongue squamous cancer cell symbolic molecules CK 8, 18 and 19; and detecting adherent cells through a western blot test to express proteins of the tongue squamous cancer cell symbolic molecules CK 8 and 18, therefore, proving on a molecular level the successful establishment of the CTSC-1.

The invention relates to the technical field of preparation of colpitis medicines, and especially relates to a vaginal gel and a preparation method thereof in order to solve the technical problem of unsatisfactory bacteriostasis, lubrication and moisture retention effects of existing vaginal gel products. The vaginal gel mainly comprises sodium hyaluronate, vitamin E succinate modified hyaluronic acid, EGF (epidermal growth factors), vitamins C, calcium chloride, pentanediol, glycerin, Rhodicare and a buffer system adopting disodium hydrogen phosphate-citric acid as gel. The vaginal gel has anti-inflammatory activity, improves the wetness of vaginas, is used for treating vaginal inflammatory diseases and alleviating vaginal dryness or used as a human body lubricant, has a vaginal mucosa squamous cell layer restoration promoting effect, can improve vaginal mucosa layer thinning and restore small injuries in vaginas to recover the elasticity and the softness of the vaginal mucosa, and is suitable for colpitis and vagina dryness people.

The invention discloses a medicine composition for treating vulva squamous cell hyperplasia, and a preparation method thereof, aiming at solving the treatment problem of vulva squamous cell hyperplasia. The medicine composition is characterized by being prepared from the following raw medicinal materials: bark of himalayan coralbean, rust-coloured crotalaria herb with root, tetrapanax papyriferum, temmoku lily magnolia, parasitic loranthus, apios fortunei max, scorched malt, scorched medicated leaven, ephedra root, leaf of mimoselike rosewood, prunus persica and cassia twig. Clinical tests prove that the medicine composition has the characteristics of good treatment effect and high safety for treating vulva squamous cell hyperplasia, and is worthy of clinic application and promotion.

The invention relates to the technical field of preparation of medicines for treating vaginitis, particularly relates to a vaginal gel containing multi-effect factors and a preparation method thereof,and solves the technical problems that the antibacterial, lubricating and moisturizing effects of the existing vaginal gel products are not ideal. The vaginal gel contains a conditioned medium extracting solution of mesenchymal stem cells and traditional Chinese medicine extract, can effectively inhibit the propagation of vaginitis-related bacteria and improve the vaginal moistness, is used for treating vaginal inflammation and relieving vaginal dry symptoms or serves as a human body lubricant, simultaneously has the effect of promoting the repair of the squamous epithelial cell layer of thevaginal mucosa, can enable the vaginal mucosa layer to be thinned and repair small injuries in the vagina, can recover the elasticity of the vaginal mucosa and enable the vaginal mucosa to restore elasticity and softness, and is suitable for people suffering from vaginitis and colpoxerosis.

The invention discloses construction of a detection classification model of pathological squamous epithelial cells. The construction comprises construction of an abnormal cell detection model, construction of a view diagram judgment model and construction of a sample judgment model. The three models in the detection and classification model are linked with one another and progressive layer by layer, the result is subjected to optimization detection, detail re-diagnosis, integration re-diagnosis and the like on the detection basis of the previous model, multiple control over the diagnosis result on the whole is achieved, the accuracy of the diagnosis result is guaranteed, and a perfect sample diagnosis method is obtained.

The invention discloses an isotretinoin amido alkyl benzoate derivative, a preparation method thereof and applications thereof. The derivative has stronger inhibition and differentiation regulating effects than isotretinoin on psoriasis, acne and epithelial cell tumors including, but not limited to skin squamous epithelial cell carcinoma, stomach cancer, lung cancer, and cervical cancer; the derivative has less influence on normal tissue cells, has a certain targeting effect on inhibiting proliferating cells, and has very less side effects than isotretinoin; and the derivative has wide promising prophylaxis and treatment applications such as cornification abnormality diseases and cell abnormal proliferation including tumors, psoriasis, acne, and other cornification abnormality dermatopathy.

The invention discloses a squamous cell carcinoma SCC-TCT combined detection method. The squamous cell carcinoma SCC-TCT combined detection method comprises the following steps: sampling exfoliated cells at a cervical part, and preprocessing to obtain a sample solution; centrifugating saponin and the sample solution, taking precipitate, adding a phosphate buffer solution, uniformly mixing, and counting the total cell number; centrifugating a counted cell suspension, adding the phosphate buffer solution for freezing and thawing at least once to obtain a sample solution of which an SCC antigen is dissociated and released; performing intracellular SCC antigen enzyme-labeled determination analysis; carrying out judging and screening according to a determination result, screening out a sample higher than a critical value, and preparing the sample solution corresponding to the sample into a uniform slide on a liquid-based cytometer, fixing, performing papanicolaou staining, performing morphological cell analysis by using a microscope, and judging the morphology of a tumor cell. According to the squamous cell carcinoma SCC-TCT combined detection method, by an SCC-TCT combined detection technology, 80-90% of normal samples are screened out, so that the working efficiency of a pathological doctor can be improved.

The present invention addresses the problem of providing a new therapeutic agent that is effective against diseases involving stratified squamous epithelial cells such as dry eye syndrome. The presentinvention is a stratified squamous epithelial cell normal differentiation and maturation promoting agent that comprises secretions of mesenchymal stem cells. It is preferable that the secretions notinclude animal serum, that the mesenchymal stem cells be adipose-derived mesenchymal stem cells, umbilical cord-derived mesenchymal stem cells, or bone marrow-derived mesenchymal stem cells, and thatthe stratified squamous epithelial cells be at least one selected from the group consisting of corneal epithelial cells, conjunctival epithelial cells, epidermal keratinocytes, oral epithelial cells,epiglottic epithelial cells, esophageal epithelial cells, vaginal epithelial cells, vocal fold epithelial cells, nasal epithelial cells, and nasal vestibular epithelial cells.

There is provided a method of testing squamous epithelial cells and a method of screening therapeutic agents for squamous cell carcinoma. That the base guanine at position 2128 (exon 17) of the FGFR3 gene is mutated to thymine in the cells of squamous cell carcinoma, or that the cells of squamous cell carcinoma produce an FGFR3 protein in which the amino acid glycine at position 697 is mutated to cysteine is utilized to facilitate the diagnosis and treatment of squamous cell carcinoma.

The invention belongs to the technical field of computer applications, and discloses a method of analyzing and testing cervical squamous epithelial cell parameters based on computer images. The methodof analyzing and testing the cervical squamous epithelial cell parameters based on the computer images includes the steps of testing colorimetrical and geometrical parameters of cervical squamous epithelial cells, and calculating textural parameters; comparing and analyzing test results, and screening the parameters with diagnostic value; discriminating and analyzing the parameters with the diagnostic value step by step, establishing a discriminant function, and evaluating the discriminant effect of the function. According to the method of analyzing and testing cervical squamous epithelial cell parameters based on the computer images, a computer medical image analysis system can be used for objectively and quantitatively describing morphological structure changes of the cells, the subjectivity of traditional qualitative observations can be overcome, the positive rate of radiograph reading is improved, the rate of missed diagnosis and misdiagnosis is decreased, the development of large-scale cancer prevention screening is facilitated, the accuracy of pathological type classification, diagnosis or prognosis judgment is improved, and the detection rate and cure rate of cervical cancer are effectively increased.

The invention provides a SCC (squamous-cell carcinoma) antigen chemiluminescence immunoassay kit and a preparation method thereof, and belongs to the technical field of in-vitro detection. The kit comprises a streptavidin magnetic particle suspension, an SCC antigen monoclonal antibody labelled by a chemiluminescent label and a coupling labelled SCC antigen antibody. The invention further providesa preparation method of the SCC antigen chemiluminescence immunoassay kit. The kit is a fully-automatic measurement sample, and directly gives numerical values, so that manual operation errors are reduced, unattended operation is realized, time required for clinical detection is shortened, detection precision is higher, a closed system is formed by a reagent and an instrument, and system errors are small.

The invention discloses an isotretinoin amido alkyl benzoate derivative, a preparation method thereof and applications thereof. The derivative has stronger inhibition and differentiation regulating effects than isotretinoin on psoriasis, acne and epithelial cell tumors including, but not limited to skin squamous epithelial cell carcinoma, stomach cancer, lung cancer, and cervical cancer; the derivative has less influence on normal tissue cells, has a certain targeting effect on inhibiting proliferating cells, and has very less side effects than isotretinoin; and the derivative has wide promising prophylaxis and treatment applications such as cornification abnormality diseases and cell abnormal proliferation including tumors, psoriasis, acne, and other cornification abnormality dermatopathy.

The present invention addresses the problem of providing a novel therapeutic agent that is effective against diseases involving stratified squamous epithelial cells such as dry eye syndrome. The present invention is an agent for promoting normal differentiation / maturation of stratified squamous epithelial cells, which comprises a secretion of mesenchymal stem cells. It is preferable that the secretions not include animal serum, that the mesenchymal stem cells be adipose-derived mesenchymal stem cells, umbilical cord-derived mesenchymal stem cells, or bone marrow-derived mesenchymal stem cells, and that the stratified squamous epithelial cells be at least one selected from the group consisting of corneal epithelial cells, conjunctival epithelial cells, epidermal keratinocytes, oral epithelial cells, epiglottic epithelial cells, esophageal epithelial cells, vaginal epithelial cells, vocal fold epithelial cells, nasal epithelial cells, and nasal vestibular epithelial cells.

The invention provides a traditional Chinese medicinal composition for treating woman vulva squamous epithelial cell proliferation and a preparation method and an application thereof. The traditional Chinese medicinal composition is prepared by using phellodendron, fructus forsythia, lawsonia inermis, Leucas mollissima chinensis and the like as main raw materials according to a certain weight ratio. the medicine provided by the invention has functions of clearing heat, removing dampness, eliminating patch, relieving itching, depriving wetness, dispelling the wind, sterilizing, promoting blood circulation to remove meridian obstruction, resolving hard lump, warming the kidney, nourishing yin, preventing precancerous lesions and the like. Multiple drugs also have synergism. The traditional Chinese medicinal composition has advantages of reasonable medication, scientific compatibility of medicines, low recurrence rate, high cure rate, good prostecdtive efficacy and no side-effect for external use. The application of the traditional Chinese medicinal composition is better than other current treatment methods.

The invention belongs to the technical field of biomedical detection and particularly relates to a marker for cervical cancer detection and a detection kit. The marker comprises a squamous cell carcinoma antigen, a carcino-embryonic antigen, a carbohydrate antigen 125, a carbohydrate antigen 199 and a human epididymal protein 4, acridinium ester is adopted for direct chemiluminescence, an antibody (antigen) is directly labeled with acridinium ester, after immunoreaction with a corresponding antigen (antibody) of a sample to be detected, a coated antibody-antigen to be detected-acridinium ester labeled antibody compound is formed, excitation liquid is added, and decomposition and luminescence can be achieved without a catalyst. A combination of multiple tumor markers is established, detection specificity and sensitivity of cervical cancer are improved through combined detection of multiple indexes, detection sensitivity and accuracy are high when serum detection is carried out on a cervical cancer patient, combined detection can have a high detection rate on tumors, and early screening, treatment effect and postoperative recurrence of cervical cancer patients can be dynamically monitored.

A spontaneously immortalized human keratinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. The method comprises the steps of obtaining a human stratified squamous epithelial cell culture, wherein the culture comprises human malignant squamous epithelial cells and spontaneously immortalized human keratinocytes, wherein the culture forms a reconstituted epidermis. One then treats the epidermis with a test tumor cell modulation agent and evaluates the growth of the malignant cells within the epidermis.

The invention provides a squamous cell carcinoma antigen detection reagent. The squamous cell carcinoma antigen detection reagent comprises magnetic microspheres coating squamous cell carcinoma antigen antibodies, squamous cell carcinoma antigen antibodies labeled by alkaline phosphatase, and a chemiluminescent substrate; and the magnetic microspheres are silicon dioxide magnetic composite particles. The squamous cell carcinoma antigen detection reagent provided by the invention has the advantages of simple components and high detection sensitivity, and can realize rapid and accurate detectionin a short time.

The invention belongs to the technical field of cell engineering, and particularly discloses a method for constructing a mammalian esophageal squamous epithelial immortalized cell line, the constructed cell line and an organoid cultured by the constructed cell line. The construction method comprises the following steps of: taking primary esophageal squamous epithelial cells as host cells, infecting the host cells by cDNA (hTERT cDNA) retrovirus containing hTERT genes, and screening by selecting a culture medium to obtain the immortalized 'normal' cell line. Meanwhile, the cell line can be cultured into a normal esophageal squamous epithelial organoid, and provides a research basis for researching development and growth of mammalian esophagus, stem cell maintenance, epithelial proliferation and differentiation homeostasis and esophageal diseases, particularly pathogenesis and therapeutic drug research and development of esophageal squamous carcinoma (ESCC).

The present invention provides a Chinese tongue squamous epithelial cell carcinoma cell line CTSC-2 establishment method so as to provide material basis for researches on characteristics and treatment drugs of human tongue squamous epithelial cell carcinoma. The method comprises the following specific steps that: a tissue mass adherence method is adopted to carry out primary culture of tongue squamous epithelial cell carcinoma cells; subculture is performed when the cell density achieves about 80%; RT-PCR detection is performed on the stably-passaging cells to show that the cells express marker molecules CK8, 18 and 19 of the human tongue squamous epithelial cell carcinoma cells; and an immunoblotting experiment is adopted to detect expression of proteins of the marker molecules CK8 and 18 of the human tongue squamous epithelial cell carcinoma cells by the adherent cells so as to prove successful establishment of the Chinese tongue squamous epithelial cell carcinoma cell line CTSC-2 from the molecular level.

The invention provides a traditional Chinese medicine capsule for treating cervical erosion and a preparation method of the traditional Chinese medicine capsule. The traditional Chinese medicine capsule is prepared from the following traditional Chinese medicine raw materials: amur corktree bark, ginkgo seed, wild chrysanthemum, myrrh, mongolian dandelion herb, common scouring rush herb, coix seed, nutgrass galingale rhizome, cattail pollen, uncaria gambir, frankincense, radix paeoniae rubra, lightyellow sophora root, peppermint, fructus persicae immaturus, tuber stemona root, ailanthus altissima swingle, golden thread, root of Chinese barberry, Japanese pagodatree juvenile branchlet, grassleaf sweelflag rhizome, indigo naturalis, Chinese atractylodes rhizome, Taiwan angelica root, dried tangerine peel, Indian buead, motherwort herb and plantain seed. The traditional Chinese medicine capsule for treating cervical erosion provided by the invention consists of pure traditional Chinese medicine raw materials, is scientific and reasonable in compatibility, has the effects of clearing away heat and drying dampness, inhibiting bacteria and killing insects, diminishing inflammation and relieving pain, promoting blood circulation and removing blood stasis, and removing slough and promoting tissue regeneration by virtue of the combination of various medicaments, can be used for accelerating the fall-off of necrotic tissues of a cervical erosion surface, promoting the effective circulation of cervical blood vessels, and promoting the regeneration of squamous epithelial cells so as to achieve the effect of stopping bleeding, repairing and healing on the cervical erosion surface, has good therapeutic effects on cervical erosion caused by various pathogens, and has significant curative effects.

The invention provides an establishing method of a Chinese tongue squamous cancer cell line (CTSC-2), and provides a material base for researching the characteristics of tongue squamous cancer and therapeutics thereof. The establishing method comprises the steps of: carrying out primary culture on the tongue squamous cancer cells by adopting a tissue explants adherent method; subculturing when the cell density reaches about 80 percent; carrying out RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection on stably subcultured cells to indicate that the cells express tongue squamous cancer cell symbolic molecules CK 8, 18 and 19; and detecting adherent cells through a western blot test to express proteins of the tongue squamous cancer cell symbolic molecules CK 8 and 18, therefore, proving on a molecular level the successful establishment of the CTSC-2.

The invention provides an establishment method of a Chinese people tongue squamous epithelial cell cancer cell line and provides a material basis for research on characteristics and treatment drugs of people tongue squamous epithelial cell cancers. The establishment method comprises specific steps as follows: primary culture is performed on tongue squamous epithelial cell cancer cells with a tissue explant adherent method; subculture is performed when the cell density reaches about 80%; RT-PCR (reverse transcription-polymerase chain reaction) detection indicates that cells which are stable in subculture express people tongue squamous epithelial cell cancer cell indicative molecules CK8, 18 and 19; and western blot experiment detection indicates that adherent cells express people tongue squamous epithelial cell cancer cell indicative molecule CK8 and 18 proteins, so that the successful establishment of the Chinese people tongue squamous epithelial cell cancer cell line CTSC-1 is proved at the molecular level.

It is intended to provide a method of evaluating a skin sensitivity level by using the expression of squamous cell carcinoma related antigen (SCCA) in skin horny layer cells as an indication.

The invention discloses a traditional Chinese medicine for treating squamous cell carcinoma. The traditional Chinese medicine consists of the following Chinese medicinal herbs by weight: 50g of wild heartleaf houttuynia herb, 50g of wild jujube tree root, 50g of walnut tree root, 20g of arisaema, 20g of kelp, 20g of peach kernel, 20g of safflower, 30g of frankincense, 20g of pseudo-ginseng, 10g of dragon's blood, 20g of ginseng, 30g of dodder, 30g of rehmannia root, 10g of dried toad skin, 10g of pilose antler, 30g of tuckahoe and 15g of licorice root, wherein the three tree roots are stir-fried with vinegar and dried and are prepared into fine powder with the rest Chinese medicine herbs to prepare capsules. The medicaments are mainly used for treating the squamous cell carcinoma, including carcinoma of larynx, carcinoma of tongue, nasopharyngeal carcinoma, carcinoma of rectum, carcinoma of skin, carcinoma of lung, esophageal carcinoma, cervical carcinoma and vulvar squamous cell carcinoma. The traditional Chinese medicine is economical, practical, safe and reliable, can be used for effectively controlling progress of disease in a short time, changing pain symptom, curing patients of early or middle period and prolonging survival life of the patients of later period. The total effectiveness ratio is 100 percent.

The invention discloses a cell carcinoma antigen hybridoma cell strain and an application method of a monoclonal antibody of the cell carcinoma antigen hybridoma cell strain. The antibody for detecting SCC is an antigen polypeptide sequence specifically designed according to the sequence characteristics of SCCA1 and SCCA2, and is used as an antigen to immunize BALB / c mice, a hybridoma cell strain4H8 for generating the antibody is obtained by using a cell fusion technology, and the antibody secreted by hybridoma cells is IgG1 positive; the invention provides the hybridoma cell strain secretingthe monoclonal antibody, the preservation number of the hybridoma cell strain is CCTCC NO: C2019315, and the hybridoma cell strain can stably secrete a high-titer monoclonal antibody for resisting squamous cell carcinoma antigen. An immune reagent developed by using the squamous cell carcinoma antigen-antibody provided by the invention can accurately determine squamous cell carcinoma antigens ina serum sample, can simultaneously detect SCCA1 and SCCA2, has high sensitivity and accuracy, has high correlation with detection results of imported reagents sold on the market, and can replace imported antibodies, so that the production cost is reduced.