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Marker and detection kit for cervical cancer detection

A detection kit and cervical cancer technology, applied in the field of biomedical detection, can solve the problem of low specificity and sensitivity of tumor marker detection, and achieve high detection sensitivity and accuracy, high detection rate and good accuracy Effect

Pending Publication Date: 2021-09-24
烟台攀崖生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specificity and sensitivity of single tumor marker detection are not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Preparation of antibody-coated magnetic particles

[0048] 1.1 Preparation of magnetic particles coated with mouse anti-SCC antibody

[0049] (1) Take 200 μL of magnetic bead solution (50 mg / mL), wash twice with 0.01M PBS buffer (p H=7.4), and finally resuspend the magnetic beads with 1 mL of PBS buffer;

[0050] (2) Add 100 μL of EDC solution to the magnetic bead solution obtained in step (1), vortex and mix well, then shake and react at room temperature for 2 hours; the room temperature is 15-40°C, which is 25°C in this example; EDC solution : 0.01M MES ((2-(N-morpholino)ethanesulfonic acid)) (PH=6.0) solution to prepare a 5mg / ml EDC solution (ready for immediate use), protected from light for later use;

[0051] (3) Discard the supernatant, wash 3 times with 0.01M PBS buffer (p H=7.4); resuspend the magnetic beads with 1mL PBS buffer;

[0052] (4) Add 50-800 μg of purified and concentrated mouse anti-SCC monoclonal antibody (400 μg in this example), vortex and m...

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Abstract

The invention belongs to the technical field of biomedical detection and particularly relates to a marker for cervical cancer detection and a detection kit. The marker comprises a squamous cell carcinoma antigen, a carcino-embryonic antigen, a carbohydrate antigen 125, a carbohydrate antigen 199 and a human epididymal protein 4, acridinium ester is adopted for direct chemiluminescence, an antibody (antigen) is directly labeled with acridinium ester, after immunoreaction with a corresponding antigen (antibody) of a sample to be detected, a coated antibody-antigen to be detected-acridinium ester labeled antibody compound is formed, excitation liquid is added, and decomposition and luminescence can be achieved without a catalyst. A combination of multiple tumor markers is established, detection specificity and sensitivity of cervical cancer are improved through combined detection of multiple indexes, detection sensitivity and accuracy are high when serum detection is carried out on a cervical cancer patient, combined detection can have a high detection rate on tumors, and early screening, treatment effect and postoperative recurrence of cervical cancer patients can be dynamically monitored.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a marker and a detection kit for cervical cancer detection. Background technique [0002] Tumor is a kind of disease that seriously threatens human health and has high morbidity and mortality. Cervical cancer is a female malignant tumor with high morbidity and mortality. According to the World Health Organization (WHO), there are 530,000 new cases every year, and about 250,000 women die from cervical cancer, of which developing countries account for 80% of the world's total. Because the lesion of cervical cancer is hidden, it is not easy to find early clinically. When patients come to see a doctor, the disease stage is often too late, which affects the treatment and curative effect. The 5-year survival rate is only 3.2% to 13%. Early diagnosis and treatment of cervical cancer are acceptable. The late treatment and prognosis are unsatisfactory. And in r...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57473G01N33/57411G01N33/57488G01N33/57496
Inventor 卢英
Owner 烟台攀崖生物技术有限公司
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