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Vaginal secretion staining fluid, and preparation method and staining method thereof

A technique for vaginal secretions and staining solution, applied in the field of cell staining, which can solve the problems of long staining time, unfavorable large-scale clinical application, cumbersome staining solution operation steps, etc., to shorten the staining time, speed up the screening speed, and improve the staining efficiency Effect

Active Publication Date: 2015-11-18
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a vaginal secretion dyeing solution and its preparation method and dyeing method, aiming to solve the existing dyeing solution with cumbersome operation steps, long dyeing time, and unfavorable large-scale Questions about clinical application

Method used

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  • Vaginal secretion staining fluid, and preparation method and staining method thereof
  • Vaginal secretion staining fluid, and preparation method and staining method thereof
  • Vaginal secretion staining fluid, and preparation method and staining method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044]Weigh 1g of neutral red, 0.125g of methylene blue, 0.1mL of glycerol, 0.01g of SDS, 10mL of absolute ethanol, 90mL of deionized water, and dissolve the dye with an electronic balance, and place it in the dark for 24 to 48 hours, and filter for later use.

Embodiment 2

[0046] Weigh 1g of neutral red, 0.1g of methylene blue, 0.025g of Azure II, 0.1mL of glycerin, 0.01g of SDS, 10mL of absolute ethanol, 90mL of MES buffer, and dissolve the dye in the dark for 24 to 48 hours with an electronic balance, and filter for later use.

Embodiment 3

[0048] Weigh 1g of neutral red, 0.125g of methylene blue, 0.1mL of glycerin, 0.1g of PVP, 0.01g of SDS, 10mL of absolute ethanol, 90ml of Bistris buffer, and dissolve the dye in the dark for 24 to 48 hours with an electronic balance, and filter for later use.

[0049] figure 1 It is the dyeing effect picture of the traditional dyeing method; Figure 2~Figure 9 It is a dyeing effect diagram of the dyeing method of the present invention. The result analysis found that: compared with the traditional staining method, the dyed image of the present invention is clearer and easier to identify, and can clearly identify components such as epithelial cells, clue cells, white blood cells, red blood cells, Candida albicans, and trichomonas. Therefore, the dyeing effect of the present invention is better than the traditional dyeing effect.

[0050] In summary, the vaginal secretion dyeing solution and its dyeing method provided by the present invention, the present invention is due to th...

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Abstract

The invention discloses a vaginal secretion staining fluid, and a preparation method and a staining method thereof. According to the preparation method, a blue basic dyestuff and a red basic dyestuff are mixed so as to obtain the vaginal secretion staining fluid, so that the vaginal secretion staining fluid possesses staining effects of universal staining fluid, staining time can be shortened greatly, staining time is shortened from general 5-30min to 20s, staining efficiency is increased greatly, and screening speed of clinical samples is accelerated. And in addition, imagines obtained via staining with the vaginal secretion staining fluid are clear, and are convenient for identification; surface layer squamous epithelial cells, middle layer epithelial cells, bottom layer epithelial cells, clue cells, leukocytes, erythrocytes, monilia albicans, and trichomonad can be identified clearly; and the vaginal secretion staining fluid is suitable for large-scale sample clinical screening.

Description

technical field [0001] The invention relates to the technical field of cell staining, in particular to a vaginal secretion staining solution, a preparation method and a staining method thereof. Background technique [0002] The chromatin in the nucleus is mainly deoxyribonucleic acid (DNA). In the DNA double helix structure, the phosphate groups on the two strands are facing outwards, negatively charged, and easily bonded with positively charged basic dyes by ionic bonds or hydrogen bonds. Bind, thereby attaching the corresponding color to the chromatin. The nucleus is the storage place of chromatin. If the chromatin is dense, the nucleus will be darkly stained; if the chromatin is loose, the nucleus will be lightly stained. The isoelectric point PI of the protein in the cytoplasm of the secretion is 3-6. When the pH>PI, the free carboxyl groups in the protein increase, which can be combined with the positively charged basic dye, so that the cytoplasm is attached to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
Inventor 赵妍何浩会
Owner DIRUI MEDICAL TECH CO LTD
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